Epidemiological survey of bovine gammaherpesvirus 4 (BoHV-4) infection in cattle and buffalo from Pakistan

Khalid, Anees; Riaz, Abid; Yousaf, Arfan; Khan, Irtaza Hassan; ur-Rehman, S.; Moaeen‐ud‐Din, Muhammad; Li, Song; Tang, Cheng; Shah, Muhammad Ali; Murtaz-Ul-Hasan,

doi:10.1007/s11259-022-10058-x

Cited by 3 publications

(1 citation statement)

References 25 publications

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“…It incorporates non-invasive methodologies that impose minimal stress on animals, thus providing a more ethical alternative. Milk antibody tests are widely used in the detection of many diseases, such as bovine tuberculosis [23], Q fever [30], Mycoplasmopsis bovis [31], and the bovine gammaherpesvirus 4 [32]. These findings coalesce to underscore the utility of a milk-based ELISA as a productive and viable alternative to serum-based assays for detecting MAP and bTB in dairy cattle.…”

Section: Discussionmentioning

confidence: 99%

Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China

Chen,

Hou,

Khalid

et al. 2024

Veterinary Sciences

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Mycobacterium avium subsp. paratuberculosis (MAP) is responsible for the persistent infectious illness known as bovine paratuberculosis, which is one of the most easily overlooked diseases in China amid a lack of epidemiological data. In this study, we evaluated the agreement of milk and blood antibody tests for paratuberculosis and showed an overall agreement of 92.0%, with a 95.0% negative coincidence rate and a 78.6% positive coincidence rate. The milk test was then used to examine the prevalence and incidence of dairy cows in Hubei Province, China. We found that, at the individual level, the highest lacto-prevalence reached up to 22.9%; the farm-level prevalence was as high as 92.3% (12/13) and 84.6% (11/13) in January and April 2018, respectively. The total incidence risk of all farms was 6% per three months. We also found that large-scale farms had a significantly lower prevalence and incidence than small-scale farms. Finally, the correlation between paratuberculosis and milk quality was evaluated, and we confirmed that MAP can significantly alter milk quality and raise somatic cell counts in the milk. This study provides valuable information for assessing the prevalence and incidence risk of paratuberculosis in China. It further provides an essential basis for calling for the prevention and control of paratuberculosis in China.

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Section: Discussionmentioning

confidence: 99%

Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China

Chen,

Hou,

Khalid

et al. 2024

Veterinary Sciences

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Identification and phylogenetic analysis of bovine herpesvirus 4 isolated from aborted bovine foetuses in Türkiye

Oz,

Dogan,

Akbaba

et al. 2024

VirusDis.

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Unveiling the Role of Bovine Herpesvirus Type 4 (BHV‐4) in Dairy Cow Reproductive Disorders: Insights From a Multifaceted Study in China

Zhu,

Dai,

Lai

et al. 2024

Transboundary and Emerging Diseases

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Bovine herpesvirus type‐4 (BHV‐4) belongs to the gamma‐herpesvirus subfamily, and its association with reproductive disorders in dairy cows is controversial. In this study, 386 whole blood and reproductive swab samples from dairy cows across eight provinces in China were collected. BHV‐4 antibody levels in the serum were determined via ELISA, while real‐time fluorescent quantitative PCR (qPCR) was employed to detect the DNA of BHV‐4, BHV‐1, bovine viral diarrhea virus type‐I (BVDV‐1), and bovine viral diarrhea virus type‐II (BVDV‐2) in the samples. Additionally, the DNA content of BHV‐4 in various tissues of cows and stillborn fetuses was assessed via qPCR. Breeding information for the participating cows was gathered through farmer interviews, and correlation analyses were conducted between the detection results and breeding information. The findings revealed a BHV‐4 antibody positive rate of 57.8% and a nucleic acid positive rate of 36.3%. Chi‐square analysis revealed a correlation between BHV‐4 and BHV‐1 infections. Among several pathogens associated with reproductive disorders, only BHV‐4 was significantly correlated, whereas BHV‐1, BVDV‐1, and BVDV‐2 were not correlated. Furthermore, the study revealed elevated BHV‐4 DNA in the reproductive tracts of cows and stillborn fetuses. Histopathological sections revealed mucosal damage in the vaginal and uterine tissues of stillborn fetuses, a phenomenon not previously reported. In summary, our study provides novel insight into the correlation between BHV‐4 and reproductive disorders and presents new evidence that supports the vertical transmission of BHV‐4.

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Epidemiological survey of bovine gammaherpesvirus 4 (BoHV-4) infection in cattle and buffalo from Pakistan

Cited by 3 publications

References 25 publications

Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China

Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China

Identification and phylogenetic analysis of bovine herpesvirus 4 isolated from aborted bovine foetuses in Türkiye

Unveiling the Role of Bovine Herpesvirus Type 4 (BHV‐4) in Dairy Cow Reproductive Disorders: Insights From a Multifaceted Study in China

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