Epidemiological survey of &lt;i&gt;Toxoplasma gondii&lt;/i&gt; and &lt;i&gt;Neospora caninum&lt;/i&gt; infections in dairy goats in Central-Southern Taiwan

Chiang, Shou-Heng; Huang, Han Hsiang; Chou, Chi-Chung; Chu, Chishih; Shih, Wen‐Ling; Lai, Jyh-Mirn; Lin, Heng-Ching; Yang, Wei-Cheng; Lee, Hsu-Hsun; Tsai, Yi-Lun; Su, Yao-Chi

doi:10.1292/jvms.20-0116

Cited by 10 publications

(12 citation statements)

References 35 publications

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“…It is well-known that bovine neosporosis is an abortigenic parasitic disease that causes severe economic losses on cattle farms. However, the economic and epizootiologic importance of Neospora caninum infection in goats remains in doubt as the pathogenesis of caprine neosporosis is mostly unknown [ 30 ]. Until the present work, there have been no reports of neosporosis in goats from Ecuador; 21–23% of seroprevalence values were shown in cattle herds from Santo Domingo, Cañar and Azuay provinces [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

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Apicomplexans in Goat: Prevalence of Neospora caninum, Toxoplasma gondii, Cryptosporidium spp., Eimeria spp. and Risk Factors in Farms from Ecuador

Celi

Guzmán

Rey-Valeirón

2022

Animals

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Neospora caninum, Toxoplasma gondii, Cryptosporidium and Eimeria cause severe impacts on the productivity of goat herds. The objectives of the present study were to establish the prevalence of these apicomplexans in goat farms from Ecuador; to evaluate a rapid test for Cryptosporidium diagnosis and to identify the risk factors associated with the infections. A questionnaire was designed to obtain information from 24 goat farms from Zapotillo, Garza Real, Cazaderos, Limones and Paletillas parishes in Ecuador. Blood (n = 388) and feces (n = 391) samples were collected. Indirect ELISA and standard parasitological assays were carried out to evaluate the seroprevalence of N. caninum and T. gondii and to detect oocysts of Cryptosporidium and Eimeria. The overall prevalence values of N. caninum and T. gondii were 12.11% and 18.20%, Cryptosporidium spp. and Eimeria spp. oocysts were detected in 10.49% and 89.51% of the total samples. A low correlation value was found between the results obtained by Ziehl-Nielsen and the rapid test. The multinomial logistic regression analysis revealed that vitamin supplementation, age of diarrhea, frequency of deworming, pasture area, presence of artiodactyls, domestic fowl, administration of sulfas, age group, body condition, abortions, type of pastures and the presence of cattle were risk factors according to the parasite species.

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Section: Discussionmentioning

confidence: 99%

“…Due to the high frequency of domestic dogs (95.8%), the odds ratio could not be determined. Canids are the final hosts of N. caninum , so their presence would be expected to be a risk factor for goats [ 30 , 41 ]. Thus, this variable should not be ruled out as a risk factor for N. caninum .…”

Section: Discussionmentioning

confidence: 99%

Apicomplexans in Goat: Prevalence of Neospora caninum, Toxoplasma gondii, Cryptosporidium spp., Eimeria spp. and Risk Factors in Farms from Ecuador

Celi

Guzmán

Rey-Valeirón

2022

Animals

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“…At that time, the multispecies ELISA kit used in this study was the only assay available to us. Although this ELISA kit has been reported to detect Toxoplasma infection in several species [12][13][14], this study represents the first time it has been used to detect Toxoplasma infection in over 70 species of animals in a single study. Another serological assay should be conducted to reconfirm the results of the multispecies ELISA assay in this study, and fortunately, we were able to obtain a commercial LAT kit in early 2021.…”

Section: Discussionmentioning

confidence: 99%

“…During the past decades, several studies have investigated Toxoplasma infection in animals in Taiwan. These studies included studies in companion animals [21][22][23], reproductive animals [14,24], wild birds [25] and Zoo animals [19,20]. Most of these studies used serological methods to investigate the prevalence of Toxoplasma infection, except for one study that used semi-nPCR to detect Toxoplasma DNA in frozen specimens from Zoo animals [20].…”

Section: Discussionmentioning

confidence: 99%

Investigation of Toxoplasma infection in zoo animals using multispecies ELISA and GRA7 nested PCR

et al. 2022

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Background Toxoplasma is an obligate intracellular protozoan that causes an important zoonotic disease with a worldwide distribution. Felids are the definitive hosts of this parasite, while virtually all warm-blooded animals, including birds, serve as intermediate hosts. Four ring-tailed lemurs (Lemur catta) in the Taipei Zoo died of acute Toxoplasma infection in June 2019. Since then, Toxoplasma has occasionally been identified in this Zoo during necropsy of dead animals and PCR of animal blood samples. Therefore, a general survey of Toxoplasma infection in animals in the Zoo seems to be needed. Methods and results An indirect multispecies ELISA was used for the first time to screen for Toxoplasma infection in 326 serum samples collected from 75 species of animals. The infection rate of Toxoplasma was 27% (88/326). A commercial latex agglutination (LAT) assay was used to re-examine the samples with doubtful and uncertain ELISA results (151 samples from 42 species). The infection rate increased to 36.2% (118/326), and the indirect multispecies ELISA appeared to be applicable to 31 of 75 species animals included in this study. Nested PCR assays targeting the dense granule protein 7 (GRA7) gene and B1 gene were also used to detect Toxoplasma in DNA samples extracted from 10 liver or blood specimens from 8 animals. GRA7 gene fragments were amplified from 8 samples from 7 animals, while B1 gene fragments were amplified from only 4 samples from 4 animals. From the B1 nested PCR and the sequence data of GRA7 fragments amplified from infectious specimens, the animals in the Zoo were speculated to have been infected by at least three different Toxoplasma variants. Conclusions According to the serological investigation, we speculated that over one-third (36.2%) of animals in Taipei Zoo presented the infection of Toxoplasma, and the indirect multispecies ELISA we used can be applied to detect Toxoplasma infection in 31 animal species included in this study. Sequence analysis revealed that at least three Toxoplasma variants were infecting the animals of Taipei Zoo.

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“…A few studies were conducted in Bangladesh on the molecular detection of T. gondii in goats and sheep [13]. However, despite the high economic return from goat farming, this enterprise faces several problems on which toxoplasmosis is crucial [14].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh

et al. 2021

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Background and Aim: Toxoplasma gondii is a protozoan parasite that is responsible for the major cause of congenital diseases, abortion, and stillbirth in humans and farm animals. Primary infection in pregnant goats due to T. gondii leads to abortion and significant economic losses in the livestock industry. Moreover, very few studies have been performed for the identification of T. gondii from aborted fetuses of goats. The study was conducted for the molecular identification of Toxoplasma gondii from aborted fetuses of goats in Chattogram, Bangladesh. Materials and Methods: Twenty aborted fetuses of goats were collected from 52 farms in the study area. A nested polymerase chain reaction (PCR) assay targeting the B1 gene was performed, and a positive sample yield of 197 bp amplified DNA products consistent with T. gondii. Results: The overall prevalence of toxoplasmosis in the aborted fetus of goats was 35.0%. Heart muscle, liver, brain, and placenta showed positive PCR results. The risk factors related to the does age, presence of cats in farms, and aborted fetus age were found to be statistically significant (p<0.05). Our results showed that T. gondii is a major possible causal factor for abortion and reproductive failure in goats. The high prevalence of T. gondii infection in aborted fetuses of goats revealed that T. gondii could be imperative in causing reproductive failure in goats. Conclusion: Active or congenital toxoplasmosis was shown by the presence of T. gondii in fetal tissues, which is a matter of concern as this parasite has zoonotic significance and causes economic hazards to the livestock industry by causing various reproductive problems. Therefore, proper control measures and strategies are needed to reduce the rate of abortion in goats, ultimately saving the livestock industry.

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Epidemiological survey of <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> infections in dairy goats in Central-Southern Taiwan

Cited by 10 publications

References 35 publications

Apicomplexans in Goat: Prevalence of Neospora caninum, Toxoplasma gondii, Cryptosporidium spp., Eimeria spp. and Risk Factors in Farms from Ecuador

Apicomplexans in Goat: Prevalence of Neospora caninum, Toxoplasma gondii, Cryptosporidium spp., Eimeria spp. and Risk Factors in Farms from Ecuador

Investigation of Toxoplasma infection in zoo animals using multispecies ELISA and GRA7 nested PCR

Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh

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