Épidémiologie des infections nosocomiales à Pseudomonas aeruginosa, Burkholderia cepacia et Stenotrophomonas maltophilia

Berthelot, Philippe; Grattard, Florence; Mallaval, F.; Ros, A.; Lucht, F.; Pozzetto, Bruno

doi:10.1016/j.patbio.2004.09.006

Cited by 23 publications

(19 citation statements)

References 96 publications

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“…Non-fermenting Gram-negative bacilli (NF-GNB) colonisation and infection are frequent among critically ill patients [1-3]. Antibiotic resistance is common in NF-GNB, resulting in inappropriate initial antibiotic treatment and poor outcomes [4-6].…”

Section: Introductionmentioning

confidence: 99%

Intensive care unit-acquired Stenotrophomonas maltophilia: incidence, risk factors, and outcome

et al. 2006

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Section: Introductionmentioning

confidence: 99%

Intensive care unit-acquired Stenotrophomonas maltophilia: incidence, risk factors, and outcome

et al. 2006

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“…According to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved from anthropogenic influence, resistance rates were lowest (amoxicillin, 1.5% and 16%; ticarcillin, 0.8% and 2.6%). Thirty-four bacteria resistant to imipenem ( n =14) or cefotaxime ( n =20) were identified as Pseudomonas aeruginosa ( n =15), Pseudomonas fluorescens (7), Stenotrophomonas maltophilia (4), Burkholderia cepacia (2), Bordetella sp. (1), Pantoea sp.…”

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confidence: 99%

“…Gram-negative bacteria in the environment, such as Enterobacteriaceae , Vibrionaceae Pseudomonadaceae , Shewanellaceae , Burkholderiaceae , Xanthomonadaceae and Moraxellaceae , were found to be resistant to major classes of antibiotics such as β-lactams and quinolones and their involvement in infectious diseases is a cause of treatment failure (4, 24). This undesirable evolution of bacteria exposes humans to health risks; indeed, aquatic environments can constitute a reservoir or a return path of microorganisms that can reach humans through the food chain (22, 26, 47) or recreational activities (31, 35).…”

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Antibiotic Resistance and Extended-Spectrum β-Lactamases in Isolated Bacteria from Seawater of Algiers Beaches (Algeria)

et al. 2012

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The aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in Algiers. The most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. According to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved from anthropogenic influence, resistance rates were lowest (amoxicillin, 1.5% and 16%; ticarcillin, 0.8% and 2.6%). Thirty-four bacteria resistant to imipenem (n=14) or cefotaxime (n=20) were identified as Pseudomonas aeruginosa (n=15), Pseudomonas fluorescens(7), Stenotrophomonas maltophilia(4), Burkholderia cepacia(2), Bordetella sp. (1), Pantoea sp. (1), Acinetobacter baumannii(1), Chryseomonas luteola(1), Ochrobactrum anthropi(1) and Escherichia coli(1). Screening for extended spectrum β-lactamase showed the presence of CTX-M-15 β-lactamase in the E. coli isolate, and the encoding gene was transferable in association with the IncI1 plasmid of about 50 kbp. Insertion sequence ISEcp1B was located upstream of the CTX-M-15 gene. This work showed a significant level of resistance to antibiotics, mainly among environmental saprophytic bacteria. Transmissible CTX-M-15 was detected in E. coli; this may mean that contamination of the environment by resistant bacteria may cause the spread of resistance genes.

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“…Pseudomonas aeruginosa causes septicemia and nosocomial infections in humans (El Amary et al, 2001;Berthelot et al, 2005) and otitis externa, pyoderma, wound infections and abscesses in canines (Griffin, 1993;Cole et al, 1998;Scott et al, 2001;Peterson et al, 2002). Ceftazidime is an important and effective antimicrobial agent for the therapy of serious infections due to multidrug resistance in P. aeruginosa.…”

mentioning

confidence: 99%

Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections

Du¹,

Kuo²,

Cheng³

et al. 2010

Vet. Med. - Czech

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Sixty-six clinical P. aeruginosa isolates, 17 obtained from canine otitis specimens and 49 received from human patients with bloodstream infections, were collected between February 2007 and January 2008. The minimal inhibitory concentrations (MICs) of the antimicrobial agents of these isolates were determined. Multidrug resistance was common, with 23 (34.8%) isolates found to be ceftazidime resistant. To explore the mechanisms of ceftazidime resistance, PCR analyses were performed to detect drug-resistance genes. The prevalence rate of Ambler class A, B, and D β-lactamase genes were obtained, with bla TEM-1 100%, bla PSE-1 100%, bla OXA-2 96.2%, bla SHV-18 91.3%, bla OXA-17 78.3%, bla VIM-3 26.1%, bla OXA-10 21.7% and bla SHV-1 8.7%. An efflux inhibition assay with the PAβN compound was conducted. The ceftazidime resistance isolates were also tested by RT-qPCR to determine the mRNA expression levels of the oprM and ampC genes. Five (21.7%) of the ceftazidime resistance isolates appeared to overactivate the OprM efflux system. The ampD, ampE, and ampR genes and the ampC-ampR intergenic region were subsequently amplified and sequenced. Five (21.7%) of the ceftazidime resistance isolates from humans and canines had a point mutation in AmpR (Asp135-Asn, n = 3; Als194-Ser, n = 2), which induces AmpC overproduction from 10-to 80-fold. This study first reported ceftazidime resistance in P. aeruginosa from canine otitis specimens, which are closely related to ESBLs (50%), including the overproduction of AmpC (25%) and the OprM efflux system (25%). The ESBLs (100%) played an important role in all ceftazidime resistance isolates from humans, and either AmpC (21.1%) or OprM (21.1%) might be overexpressed within the same isolate. A human patient isolate (H307B) showed simultaneous expression of ESBLs, the OprM efflux system, and AmpC overproduction.

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Épidémiologie des infections nosocomiales à Pseudomonas aeruginosa, Burkholderia cepacia et Stenotrophomonas maltophilia

Cited by 23 publications

References 96 publications

Intensive care unit-acquired Stenotrophomonas maltophilia: incidence, risk factors, and outcome

Intensive care unit-acquired Stenotrophomonas maltophilia: incidence, risk factors, and outcome

Antibiotic Resistance and Extended-Spectrum β-Lactamases in Isolated Bacteria from Seawater of Algiers Beaches (Algeria)

Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections

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