Epidemiology and antimicrobial resistance characteristics of the ST131-<i>H30</i>subclone among extraintestinal<i>Escherichia coli</i>collected from US children

Miles-Jay, Arianna; Weissman, Scott J.; Adler, Amanda; Tchesnokova, Veronika; Sokurenko, Evgeni V.; Baseman, Janet; Zerr, Danielle M.

doi:10.1101/122416

Cited by 1 publication

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“…It has been shown that the E.coli ST131 clone, which has become the most common extraintestinal pathogenic E. coli clone circulation today by colonizing the gastrointestinal systems of humans and food animals, is associated with ESBL production, mostly CTX-M-15, as well as multidrug resistance and treatment failure. 4,7 Among the identified subclones of ST131, H30R shows resistance to fluoroquinolones; H30Rx, on the other hand, is characterized by the production of CTX-M-15-type ESBL and members of this subclone are resistant to broad-spectrum cephalosporins, as well as fluoroquinolones. 8,9 The detection of high-risk ST131 clone in a population provides important information on the dynamics of spread of resistance.…”

Section: Introductionmentioning

confidence: 99%

Investigation of High-Risk ST131 Clone in Extended Spectrum β-Lactamase–Producing Escherichia coli Isolates in Children

Bulut¹,

Hürkal²,

Dalgıç³

2021

Journal of Pediatric Infectious Diseases

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Objective Antimicrobial resistance poses a serious threat to children's health. In recent years, high-risk Escherichia coli ST131 has become an important target for global surveillance studies. The E.coli ST131 clone is associated with extended spectrum β-lactamase (ESBL) production, as well as multidrug resistance and treatment failure. Studies on this clone in the pediatric age group are limited. We aim to investigate the rate of high-risk E. coli ST131 clone in ESBL-positive E. coli isolates obtained from pediatric patients. Methods A total of 292 ESBL-positive E. coli isolates from clinical samples of pediatric patients was included in the study. MALDI-TOF MS system was used for bacterial identification. Susceptibility tests were performed using BD Phoenix automated system. ST131 detection was done by MALDI-TOF-MS. Fisher's exact test was used to compare the groups (significance <0.05). Results A total of 292 isolates was analyzed. The high-risk ST131 clone was detected in 117 (40%) of the 292 ESBL-positive isolates. ST131 rates were found to be significantly higher in children under the age of 5 years compared with children over the age of 5 years (49.3 vs. 31.1%, p = 0.0019). Ciprofloxacin resistance was higher in ST131 isolates (45.6 vs. 31.7%; p < 0.05). Conclusion The rate of the ST131 clone was found to be high in the pediatric population. The significantly high rate of resistance to ciprofloxacin, which is not commonly used in the pediatric population, in ST131 isolates reveals the importance of the spread of high-risk clones for the development of resistance.

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