Epidemiology and Characteristics of Metallo-β-Lactamase-Producing<i>Pseudomonas aeruginosa</i>

Hong, Duck Jin; Bae, Il Kwon; Jang, In Ho; Jeong, Seok Hoon; Kang, Hyun Kyung; Lee, Kyungwon

doi:10.3947/ic.2015.47.2.81

Cited by 245 publications

(214 citation statements)

References 156 publications

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“…Moreover, eight carbapenem resistant isolates (50%) are multi-drug resistant to three classes of antimicrobial agents (Table 2). Overall, high resistance to extended-spectrum β-lactams and carbapenems were reported in P. aeruginosa worldwide [4]. A rapid dissemination of carbapenemases enzymes has been reported in different countries, including India, Pakistan, Australia, Germany, Belgium, Canada, Kenya, Sultanate of Oman, and United States [25][26].…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa isolates resistant to carbapenems

Shaaban

Al‐Qahtani

Al‐Ahdal

et al. 2018

J Infect Dev Ctries

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Introduction: Emergence of carbapenem resistance in Pseudomonas aeruginosa increases the therapeutic dilemma. In this study, we investigated various mechanisms involved in the resistance of P. aeruginosa clinical isolates to carbapenems. Methodology: P. aeruginosa isolates were isolated from different clinical samples. The antimicrobial susceptibility was evaluated by disc diffusion method. Carbapenemases were detected among carbapenem resistant isolates. Expression level of mexB and oprD was determined by real-time PCR. Molecular relatedness among isolates was detected based on pulsed-field gel electrophoresis (PFGE). Results: Ninety P. aeruginosa isolates were purified from clinical specimens. High levels of resistance to imipenem and meropenem were detected in 16 isolates. PCR analysis of carbapenemases indicated the prevalence of Verona integron-encoded metallo-beta-lactamase (VIM); four isolates produced only VIM enzymes (VIM-1 or VIM-2), while the remaining twelve co-produced both VIM-1 or VIM-2 and NDM enzymes. Additionally, real-time PCR analysis elucidated high expression levels of mexB in seven of the carbapenem resistant isolates and low expression of oprD in seven isolates. The identified carbapenem-resistant isolates were clustered into eleven PFGE profiles where clusters E1 and E2 involved isolates exhibiting multiple carbapenemase genes (blaNDM-1, blaVIM-1 and blaVIM-2). Conclusion: Various mechanisms underlying carbapenem resistance have been detected in our P. aeruginosa cohort of isolates. Emergence of P. aeruginosa as a reservoir of multiple carbapenemases is increasing over time limiting the treatment options to this serious infection. This increases the urgency for infection control practices to reduce the incidence of this infection.

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Section: Discussionmentioning

confidence: 99%

“…However, carbapenem-resistant P. aeruginosa could be resistant to other classes of antimicrobial agents and such infections are associated with limited therapeutic options and high rates of mortality and morbidity especially in hospitalized and immunocompromised patients [3][4].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa isolates resistant to carbapenems

Shaaban

Al‐Qahtani

Al‐Ahdal

et al. 2018

J Infect Dev Ctries

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“…Multidrug resistance of NFGNB stems from different factors, such as up-regulated production of enzymes metabolizing the drugs, target site changes, overexpression of efflux pumps, and porin deficiency 5,6 . Metallobeta-lactamase (MBL) production, mainly by Pseudomonas aeruginosa, stands out as a frequent cause of severe nosocomial infections 7,8 . The prevalence, phenotypic characteristics, and antimicrobial susceptibility profile of NFGNB strains may show regional variation.…”

mentioning

confidence: 99%

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Argenta

Fuentefria

Sobottka

2017

Rev. Soc. Bras. Med. Trop.

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Introduction:We compared the prevalence and antimicrobial susceptibility of non-fermenting gram-negative bacilli (NFGNB) isolated from clinical samples at a Brazilian tertiary care hospital in 2008 and 2013. Methods: Collected data included patient's name, age, sex, inpatient unit, laboratory record number, type of biological material, culture test result, and antimicrobial susceptibility of isolated strains. Results: Out of 19,112 culture tests analyzed, 926 (4.8%) were positive for NFGNB. Among these, 45.2% were metallo-beta-lactamase (MBL) producing strains. Conclusion: Between 2008 and 2013, the number of MBL-producing NFGNB isolates increased by 21.5%, which was accompanied by a consequent reduction in susceptibility to antimicrobials.

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“…lipotoxin [241] metallo-β-Lactamase antimicrobial resistance factor [242] PcrV (type III secretion system) virulence factor [243] phenazine and derivates virulence factor [244][245][246] Pseudomonas aeruginosa pseudoverdine virulence factor [247] pyochelin (siderophore) virulence factor [248] pyocyanin (phenazide) virulence factor [223,[249][250][251][252][253][254][255][256][257][258][259][260][261][262] pyoluteorin [263] pyoverdine virulence factor [227,[264][265][266] rhamnolipids bacterial surfactants [227,267] Tse2 virulence factor [268] TypA virulence factor [269] Type II secretion system virulence factor [270] Type III secretion system virulence factor [271- …”

Section: Microorganism Secondary Metabolites Commentsmentioning

confidence: 99%

Database on the taxonomical characterisation and potential toxigenic capacities of microorganisms used for the industrial production of food enzymes and feed additives, which do not have a recommendation for Qualified Presumption of Safety

Benito

Ibáñez

Moncho

et al. 2017

EFSA Supporting Publications

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The present work constitutes the external scientific report of the EFSA open call OC/EFSA/FEED/2015/01. The aim of the call was to provide EFSA with a database from a review on the taxonomical description and potential toxigenic capacities of microorganisms used for the industrial production of feed additives and food enzymes. The review includes microorganisms used as source of feed additives and food enzymes for which EFSA has received or can potentially receive applications for safety assessment, and which have not been recommended for Qualified Presumption of Safety status. The database also comprises the molecular taxonomical identifiers and biosynthetic pathways involved in the production of toxic compounds and the responsible genes. The main result of the project is shown as a database developed according to the EFSA data structure. The methodological aspects and the queries used in the systematic search, as well as the procedure applied for the screening of scientific documents retrieved are described in this report. Details are available in supplementary appendices.In total, 22970 scientific documents were screened in the literature search, from which 411 were initially selected for providing pertinent data for the scope of the project. From the review of the selected articles, 474 bioactive secondary metabolites were recorded and 59 compounds were further studied in order to obtain data on their toxicology and the conditions in which they are produced by the microorganisms used in industrial fermentations. The database generated in this project comprises details that characterise, when available, the production conditions, genes involved and toxicity of these 59 compounds. This provides information that can be used to establish safety measures when using potentially toxigenic microorganisms in industrial fermentations Disclaimer: The present document has been produced and adopted by the bodies identified above as author(s). This task has been carried out exclusively by the author(s) in the context of a contract between the European Food Safety Authority and the author(s), awarded following a tender procedure. The present document is published complying with the transparency principle to which the Authority is subject. It may not be considered as an output adopted by the Authority. The European Food Safety Authority reserves its rights, view and position as regards the issues addressed and the conclusions reached in the present document, without prejudice to the rights of the authors. Acknowledgements:We thank the following EFSA satff: Jaime Aguilera, Margarita Aguilera-Gómez, Jane Richardson, Mario Monguidi and Davide Gibin for their valuable help and collaboration throughout the project. We also would like to thank the members of AINIA: Sonia Porta, Lidia Tomás, Joaquin Espí and Juan Antonio Nieto for providing expertise in biotechnology and molecular biology and contributing with their efforts to achieve the goals of this project, and Violeta Gómez from the University of Navarra for her colla...

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Epidemiology and Characteristics of Metallo-β-Lactamase-ProducingPseudomonas aeruginosa

Cited by 245 publications

References 156 publications

Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa isolates resistant to carbapenems

Molecular characterization of resistance mechanisms in Pseudomonas aeruginosa isolates resistant to carbapenems

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Database on the taxonomical characterisation and potential toxigenic capacities of microorganisms used for the industrial production of food enzymes and feed additives, which do not have a recommendation for Qualified Presumption of Safety

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