Epidemiology and molecular characteristics of the type VI secretion system in <i>Klebsiella pneumoniae</i> isolated from bloodstream infections

Zhou, Mingmei; Lan, You; Wang, Siyi; Liu, Qingxia; Jian, Zijuan; Liu, Yanming; Chen, Xia; Yan, Qun; Liu, Wenen

doi:10.1002/jcla.23459

Cited by 11 publications

(9 citation statements)

References 46 publications

(95 reference statements)

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“…Positive results for hcp , icmF , and vgrG genes confirmed T6SS positivity ( Zhou et al, 2020 ; Liao et al, 2022 ; Zhang et al, 2022 ), and 36.1% (61/169) of our strains were T6SS-positive; this was much higher than the rate in blood stream infection (BSI) (20.1%) ( Zhou et al, 2020 ). We identified hvKp in 41.42% (70/169) of patients and hypermucoviscosity phenotype in 47.93% (81/169).…”

Section: Discussionsupporting

confidence: 66%

“…The presence of T6SS, capsular serotyping, virulence, and drug resistance genes was detected by polymerase chain reaction (PCR), as previously described ( Zhou et al, 2020 ). Genomic DNA of K. pneumoniae was extracted using the boiling method.…”

Section: Methodsmentioning

confidence: 99%

“…Genomic analysis identified that several genes coding for iron uptake are encoded around the T6SS gene; thus, T6SS could be involved in iron uptake in hvKp and may be a new virulence factor in hvKp strains ( Barbosa and Lery, 2019 ; Zhu et al, 2021 ; Altayb et al, 2022 ). Two studies reported the frequency of the T6SS gene in K. pneumoniae isolated from bloodstream infections ( Zhou et al, 2020 ; Zhang et al, 2022 ). However, the incidence rate of the T6SS gene in K. pneumoniae causing abscesses and in vivo studies of T6SS virulence has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology and clinical characteristics of the type VI secretion system in Klebsiella pneumoniae causing abscesses

et al. 2023

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PurposeThe type VI system (T6SS) has the potential to be a new virulence factor for hypervirulent Klebsiella pneumoniae (hvKp) strains. This study aimed to characterize the molecular and clinical features of T6SS-positive and T6SS-negative K. pneumoniae isolates that cause abscesses.Patients and methodsA total of 169 non-duplicate K. pneumoniae strains were isolated from patients with abscesses in a tertiary hospital in China from January 2018 to June 2022, and clinical data were collected. For all isolates, capsular serotypes, T6SS genes, virulence, and drug resistance genes, antimicrobial susceptibility testing, and biofilm formation assays were assessed. Multilocus sequence typing was used to analyze the genotypes of hvKp. T6SS-positive hvKp, T6SS-negative hvKp, T6SS-positive cKP, and T6SS-negative cKP (n = 4 strains for each group) were chosen for the in vivo Galleria mellonella infection model and in vitro competition experiments to further explore the microbiological characteristics of T6SS-positive K. pneumoniae isolates.ResultsThe positive detection rate for T6SS was 36.1%. The rates of hvKp, seven virulence genes, K1 capsular serotype, and ST23 in T6SS-positive strains were all higher than those in T6SS-negative strains (p < 0.05). Multivariate logistic regression analysis indicated that the carriage of aerobactin (OR 0.01) and wcaG (OR 33.53) were independent risk factors for T6SS-positive strains (p < 0.05). The T6SS-positive strains had a stronger biofilm-forming ability than T6SS-negative strains (p < 0.05). The T6SS-positive and T6SS-negative strains showed no significant differences in competitive ability (p = 0.06). In the in vivo G. mellonella infection model, the T6SS(+)/hvKP group had the worst prognosis. Except for cefazolin and tegacyclin, T6SS-positive isolates displayed a lower rate of antimicrobial resistance to other drugs (p < 0.05). The T6SS-positive isolates were more likely to be acquired from community infections (p < 0.05).ConclusionKlebsiella pneumoniae isolates causing abscesses have a high prevalence of T6SS genes. T6SS-positive K. pneumoniae isolates are associated with virulence, and the T6SS genes may be involved in the hvKp virulence mechanism.

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Section: Discussionsupporting

confidence: 66%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology and clinical characteristics of the type VI secretion system in Klebsiella pneumoniae causing abscesses

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“…The type VI secretion system (T6SS) could also be targeted by peptidomimetics ( Cherrak et al, 2021 ). T6SS has been associated with virulence in several bacterial pathogens, including some ESKAPE group members ( Enterococcus faecium , Staphylococcus aureus , Klebsiella pneumoniae , Acinetobacter baumannii , P. aeruginosa , and Enterobacter cloacae ), which are a serious threat for the human health due to the development of high resistance levels against a broad spectrum of antibiotics ( Boyer et al, 2009 ; Wang et al, 2015 ; Kim et al, 2017 ; Zhou et al, 2020 ). Like to NF-T3SS, T6SS is a multiprotein complex for whose assembly the protein–protein interactions could be essential ( Cherrak et al, 2021 ).…”

Section: Dedicated Secretion Systemsmentioning

confidence: 99%

Peptidomimetics as Potential Anti-Virulence Drugs Against Resistant Bacterial Pathogens

2022

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The uncontrollable spread of superbugs calls for new approaches in dealing with microbial-antibiotic resistance. Accordingly, the anti-virulence approach has arisen as an attractive unconventional strategy to face multidrug-resistant pathogens. As an emergent strategy, there is an imperative demand for discovery, design, and development of anti-virulence drugs. In this regard, peptidomimetic compounds could be a valuable source of anti-virulence drugs, since these molecules circumvent several shortcomings of natural peptide-based drugs like proteolytic instability, immunogenicity, toxicity, and low bioavailability. Some emerging evidence points to the feasibility of peptidomimetics to impair pathogen virulence. Consequently, in this review, we shed some light on the potential of peptidomimetics as anti-virulence drugs to overcome antibiotic resistance. Specifically, we address the anti-virulence activity of peptidomimetics against pathogens’ secretion systems, biofilms, and quorum-sensing systems.

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“…The first K. pneumoniae T6SS effector, a phospholipase (Pld1), was then identified and its role in pulmonary infection was demonstrated in a murine model using a pld1 deletion mutant strain 29 . More recently, analysis of 107 fully sequenced genomes of K. pneumoniae identified 254 T6SS related clusters 30 , and their presence was positively correlated with the hypervirulent phenotype 31 , 32 . T6SS are conserved worldwide across the genome of K. pneumoniae sequence type 258 (ST258) causing health-care-associated pneumonia 33 .…”

Section: Introductionmentioning

confidence: 99%

Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization

Merciecca

Bornes

Nakusi

et al. 2022

Sci Rep

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Type VI secretion systems (T6SS), recently described in hypervirulent K. pneumoniae (hvKp) strains, are involved in bacterial warfare but their role in classical clinical strains (cKp) has been little investigated. In silico analysis indicated the presence of T6SS clusters (from zero to four), irrespective of the strains origin or virulence, with a high prevalence in the K. pneumoniae species (98%). In the strain CH1157, two T6SS-apparented pathogenicity islands were detected, T6SS-1 and -2, harboring a phospholipase-encoding gene (tle1) and a potential new effector-encoding gene named tke (Type VI Klebsiella effector). Tle1 expression in Escherichia coli periplasm affected cell membrane permeability. T6SS-1 isogenic mutants colonized the highest gastrointestinal tract of mice less efficiently than their parental strain, at long term. Comparative analysis of faecal 16S sequences indicated that T6SS-1 impaired the microbiota richness and its resilience capacity. Oscillospiraceae family members could be specific competitors for the long-term gut establishment of K. pneumoniae.

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Epidemiology and molecular characteristics of the type VI secretion system in Klebsiella pneumoniae isolated from bloodstream infections

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 46 publications

Molecular epidemiology and clinical characteristics of the type VI secretion system in Klebsiella pneumoniae causing abscesses

Molecular epidemiology and clinical characteristics of the type VI secretion system in Klebsiella pneumoniae causing abscesses

Peptidomimetics as Potential Anti-Virulence Drugs Against Resistant Bacterial Pathogens

Role of Klebsiella pneumoniae Type VI secretion system (T6SS) in long-term gastrointestinal colonization

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