Epidemiology and molecular characterization of
            <i>Carnivore protoparvovirus‐1</i>
            infection in the wild felid
            <i>Leopardus guigna</i>
            in Chile

Sacristán, Carlos; Esperón, Fernando; Pérez, Ruben; Acuña, Francisca; Aguilar, Emilio de la Cruz; García, Sebastián; López, María José; Neves, Elena; Cabello, Javier; Hidalgo-Hermoso, Ezequiel; Terio, Karen A.; Millán, Javier; Poulin, Élie; Napolitano, Constanza

doi:10.1111/tbed.13937

Cited by 13 publications

(9 citation statements)

References 83 publications

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“…Feline parvovirus, CDV, and IAV were detected by real-time PCR, and FCoV was detected by nested PCR, as previously described ( Herrewegh et al, 1995 ; Meli et al, 2009 ; Streck et al, 2013 ); the PCR primers are shown in Supplementary Table 1 . VP2 gene of FPV was amplified by nested PCR ( Mochizuki et al, 1996 ; Steinel et al, 2000 ; Battilani et al, 2001 ; Sacristán et al, 2021 ). FPV and FCoV positive controls were cat samples with the corresponding disease, which were collected in pet hospitals.…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021

Huang

Xie

et al. 2022

Front. Microbiol.

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The fact that wild felines are carriers of pernicious infectious viruses should be a major concern due to the potential cross-species transmission between the felines and human or domestic animals. However, studies on the virus in the captive wild felines, especially in tigers, are thin on the ground. In this study, we screened four infectious viruses, namely, feline parvovirus (FPV), feline coronavirus (FCoV), canine distemper virus (CDV), and influenza A virus (IAV), in the blood samples of 285 captive Siberian tigers (Panthera tigris altaica) and in the spleen samples of two deceased lions (Panthera leo), which were collected from 2019 to 2021 in three Siberian Tiger Parks from the northeast of China. Nucleic acids isolated from the blood samples collected from tigers and the spleen samples collected from two deceased lions were positive for FPV by PCR, and the positive rate was 4.6% (13/285) in tigers. Furthermore, the VP2 gene of FPV was amplified by nested PCR, and the sequences of the VP2 gene from these six FPV positive strains shared 98.3–99.9% homology with the reference. The key amino acid sites of VP2 protein were consistent with that of FPV reference strains. Phylogenetic analysis based on the VP2 gene showed that in this study, FPV-positive strains were grouped within the FPV clade and closely related to the Asian strains clade. The results of this study showed that FPV circulated in the captive Siberian tigers and lions in northeastern China and provided valuable information for the study of FPV epidemiology in wild felines. Therefore, we suggest that regular antibody monitoring and booster immunization for tigers should be performed.

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Section: Methodsmentioning

confidence: 99%

Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021

Huang

Xie

et al. 2022

Front. Microbiol.

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“…However, rodents have been recognized as hosts of T. spiralis mainly in the domestic environment in Chile [23,42]. This record is in accordance with the fact that guiñas have been frequently infected by micro-organisms spilled from free-range domestic animals [43,44]; and, although T. spiralis is not an important pathogen for the health of non-human animals, its presence in the güiña highlights the need of surveyance of pathogens in the rural-sylvatic interphase.…”

Section: Discussionmentioning

confidence: 80%

Trichinella in Wildlife in Chile With the First Record in Leopardus Guigna and Galictis Cuja

Echeverry

Henríquez

González‐Acuña

et al. 2021

Preprint

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Background: Trichinellosis is a worldwide distributed disease caused by Trichinella species and also considered neglected and emerging. Trichinella spp. are transmitted by predation or carrion consumption and present a domestic and a sylvatic cycle. Human trichinellosis occurs due to the consumption of raw or poorly cooked infected meat, mainly associated with the household slaughter of pigs without veterinary inspection, a cultural practice that is difficult to resolve. Therefore, the knowledge of the reservoir of this parasite is relevant. Methods: The aim of this study was to assess the presence of Trichinella sp. in several carnivore and omnivore vertebrates in Central-Southern Chile. A total of 61 animals, either found run over or that died in rescue/rehabilitation centers, encompassing 15 species, were examined by artificial digestion for detection of Trichinella larvae, and larvae were molecularly analyzed to identify the species. Results: Trichinella larvae were found in a Leopardus guigna and a Galictis cuja. Only those of L. guigna could be identified as Trichinella spiralis. Conclusions: This is the first record of Trichinella in a native mustelid of South America and the first record of T. spiralis in L. guigna. These results increase the number of hosts, enhancing the need to identify the role of these animals in the reservoir for humans, and highlighting the priority that the study of the rural-sylvatic interphase represents.

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“…Rodents Full-size DOI: 10.7717/peerj.11601/fig- 2 have been recognized as hosts of T. spiralis, mainly in the domestic environment in Chile (Schenone et al, 1967;Schenone et al, 2002). This record is in accordance with the fact that güiñas have been frequently infected by pathogens from free-roaming domestic animals (Ortega et al, 2020;Sacristán et al, 2020); although T. spiralis is not an important pathogen for the health of non-human animals, its presence in the güiña highlights the need for pathogen surveillance in the rural-sylvatic interphase.…”

Section: Discussionmentioning

confidence: 87%

First record of Trichinella in Leopardus guigna (Carnivora, Felidae) and Galictis cuja (Carnivora, Mustelidae): new hosts in Chile

Echeverry

Henríquez

Oyarzún‐Ruiz

et al. 2021

PeerJ

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Background Trichinellosis is a zoonotic disease with a worldwide distribution. It is caused by several species of nematodes in the genus Trichinella. Trichinella spp. are transmitted through predation or carrion consumption and occur in domestic and sylvatic cycles. In humans trichinellosis occurs due to the consumption of raw or undercooked, infected meat and is mainly associated with the household slaughter of pigs or the consumption of game animals without veterinary inspection, a cultural practice that is difficult to resolve. Therefore, knowledge of this parasite’s reservoir is relevant for better implementing public health strategies. The aim of this study was to assess the presence of Trichinella sp. in several carnivore and omnivore vertebrates in central-southern Chile. Methods We collected muscle tissue from a total of 53 animals from 15 species and were digested to detect Trichinella larvae which were further identified to species level using molecular techniques. Results We detected Trichinella larvae in Leopardus guigna (Felidae) and Galictis cuja (Mustelidae). We identified the larvae collected from L. guigna as Trichinella spiralis, but we were unable to molecularly characterize the larvae from G. cuja. This is the first record of Trichinella in a native mustelid of South America and the first record of T. spiralis in L. guigna. This study identified two novel hosts; however, further work is needed to identify the role that these and other hosts play in the cycle of Trichinella in Chile.

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Epidemiology and molecular characterization of Carnivore protoparvovirus‐1 infection in the wild felid Leopardus guigna in Chile

Abstract: Article type : Original Article Epidemiology and molecular characterization of Carnivore protoparvovirus-1 infection in the wild felid Leopardus guigna in Chile Short running title: Carnivore protoparvovirus-1 at the wild-domestic interface.

Cited by 13 publications

References 83 publications

Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021

Molecular Detection of Parvovirus in Captive Siberian Tigers and Lions in Northeastern China From 2019 to 2021

Trichinella in Wildlife in Chile With the First Record in Leopardus Guigna and Galictis Cuja

First record of Trichinella in Leopardus guigna (Carnivora, Felidae) and Galictis cuja (Carnivora, Mustelidae): new hosts in Chile

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