Epidemiology of bovine babesiosis and anaplasmosis in Zambia

Jongejan, Frans; Perry, Brian D.; Moorhouse, P. D. S.; Musisi, F. L.; Pegram, R. G.; Snacken, Morgane

doi:10.1007/bf02239989

Cited by 37 publications

(30 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In many cases, they have been shown to occur as a mixed infection [25,26,32,81]. In Zambia, Babesiosis and Anaplasmosis count amongst the most important of all TBD.…”

Section: Parasites and Distributionmentioning

confidence: 99%

See 1 more Smart Citation

The current status of major tick borne diseases in Zambia

Makala

Mangani²,

Fujisaki

et al. 2003

Vet. Res.

View full text Add to dashboard Cite

-Tick-borne diseases occurring in Zambia are assuming more importance as they continue to be a major economic problem not only in Zambia, but in many parts of Eastern, Southern and Central Africa. The current control methods, which include the use of toxic acaricides to kill ticks, and the virulent sporozoite infection and treatment method have limitations. Recombinant vaccines, currently in their experimental stages, offer hope for the future. The use of acaricides is hampered by the development of acaricide resistance and live vaccines are dependent on cold chain facilities, which are a formidable obstacle in the poorly developed infrastructure in parts of Zambia where the vaccine is most needed. Amidst these drawbacks are the results of the recent research on parasites and vector recombinant vaccines which promise to circumvent these problems. The history, current status and attitudes regarding the control of these diseases, taking into account their complexity, are reviewed. The establishment of the well-designed Central Veterinary Research Institute (CVRI) and Japanese International Cooperation Agency (JICA) sponsored veterinary school, both have a potential for high quality research, with access to a wealth of specimens a veritable goldmine of research material. It is thus hoped that this review will stimulate the desire to maximize the value of the tick and tick-borne disease research in both Zambia and the international research community.

show abstract

“…In many cases, they have been shown to occur as a mixed infection [25,26,32,81]. In Zambia, Babesiosis and Anaplasmosis count amongst the most important of all TBD.…”

Section: Parasites and Distributionmentioning

confidence: 99%

“…Heartwater caused by Cowdria ruminantium is a rickettsial disease that affects domestic and wild ruminants in Zambia [32], the rest of Africa and the Carribean. The ticks, Amblyomma hebraeum and especially A. variegatum are the main vectors of heartwater in the agricultural areas of Zambia.…”

Section: Parasites and Distributionmentioning

confidence: 99%

The current status of major tick borne diseases in Zambia

Makala

Mangani²,

Fujisaki

et al. 2003

Vet. Res.

View full text Add to dashboard Cite

show abstract

“…No estudo da anaplasmose, diferentes testes sorológicos têm sido utilizados e comparações foram realizadas entre eles, como Fixação de Complemento -FC (RODGERS et al, 1994), Teste Aglutinação do Cartão -TAC (PEREZ et al, 1994), Imunofluorescência Indireta -IFI (FRAGADA; CORDOVÉS; PUENTES, 1991) e ELISA (NIELSEN et al, 1996), TAC e IFI (KNOWLES et al, 1982), FC, TAC e IFI (BUNDY et al, 1983), IFI e ELISA (JONGEJAN et al, 1988), cELISA (KNOWLES et al, 1996), cELISA e TAC (MOLLOY et al, 1999).…”

Section: Introductionunclassified

Soroprevalência de Anaplasma marginale em bovinos da região Centro-Sul do estado do Paraná, Brasil, por um teste imunoenzimático competitivo utilizando proteína recombinante MSP5-PR1

Marana¹,

Dias²,

Freire³

et al. 2009

RBPV

View full text Add to dashboard Cite

ResumoFoi determinada a prevalência de Anaplasma marginale em 223 soros de bovinos com idade maior ou igual a dois anos, das regiões de Ponta Grossa, Guarapuava e Laranjeiras do Sul, região Centro-Sul do estado do Paraná. O teste imunoenzimático de competição PR1 (cELISA-PR1) foi utilizado para determinar a presença ou ausência de anticorpos anti-A. marginale. Dos 223 soros analisados, 130 (58,74%) foram positivos pelo cELISA-PR1, sugerindo ser essa região de instabilidade de enzoótica, com uma porcentagem significativa de animais susceptíveis à infecção por A. marginale, com risco potencial para desenvolver a anaplasmose. As características de tipo de exploração da propriedade, sistema de criação, manejo e forma de comercialização dos animais foram avaliadas. A análise estatística não demonstrou haver diferença significativa entre as variáveis estudadas e a positividade dos animais.Palavras-chave: Anaplasma marginale, MSP5-PR1, cELISA, soroprevalência, bovino. AbstractAnaplasma marginale prevalence was determined in 223 sera samples in 2-year old or older cattle, from the CenterSouthern Region of the Paraná State, including Ponta Grossa, Guarapuava and Laranjeiras do Sul municipalities. A survey of antibodies IgG class against Anaplasma marginale was performed through a competitive immune absorbent assay (cELISA-PR1). From the 223 sera examined, 130 (58.74%) reacted to cELISA-PR1 test, suggesting an region of enzootic instability, with a significant percentage of animals susceptible to infection by A. marginale and potentially in risk to develop anaplasmosis. The kind of exploration in the property, the breeding and handling system, the presence of other animals (ovine/caprine, horses, wild animals), and means of commercialization of animals were analyzed. The statistical analysis showed that there were no significant differences among the analyzed variables.

show abstract

“…Entre eles, a Fixação de Complemento (RODGERS et al, 1994), Aglutinação pelo Látex (PEREZ et al, 1994), Conglutinação (ROSE et al, 1978), Imunofluorescência Indireta (JONGEJAN et al, 1988;FRAGADA et al, 1991), Ensaios Imunoenzimáticos (ELISA) (NAKAMURA et al, 1988;NIELSEN et al, 1996) e ELISA por competição (cELISA) (KNOWLES et al, 1996;TORIONI et al, 1998).…”

Section: Introductionunclassified

Clonagem, expressão, caracterizaÇão molecular da proteína de superfície MSP5 da amostra PR1 de Anaplasma marginale e sua aplicaÇão em um teste de ELISA por competiÇão

Marana¹,

Kano²,

Vicentini³

et al. 2009

RBPV

View full text Add to dashboard Cite

ResumoNo presente trabalho, um teste imunoenzimático por competição (cELISA) foi padronizado com a proteína recombinante de superfície rMSP5, clonada a partir do gene msp5 do isolado PR1 de A. marginale. O sequenciamento mostrou que o gene que codifica a rMSP5/PR1 tem 98% de identidade com os isolados Flórida e Santa Maria, 97% com isolados de Pernambuco, Brasil e Havana, Cuba e 91% com A. centrale. O teste de cELISA-PR1 foi comparado com os testes de IFI e cELISA-USA. Para a padronização e validação do cELISA-PR1, foram utilizados 380 soros bovinos comprovadamente positivos ou negativos pelo cELISA-USA. Desse total, 245 soros positivos eram de animais de área endêmica e 135 soros eram negativos, de área livre de anaplasmose. Na padronização, foram utilizados 283 soros de bovinos, dos quais 135 eram negativos e 148 positivos. Os testes de cELISA-PR1 e IFI apresentaram especificidade de 100 e 99,3%, sensibilidade de 100 e 98%, com coeficiente kappa de 0,993 e 0,978, respectivamente. Na validação do teste, foram utilizados 245 soros de bovinos de áreas endêmicas para anaplasmose, testados pelo cELISA-PR1 e IFI e apresentaram especificidade de 96,7 e 69,1%, sensibilidade de 98,9 e 96,3% e coeficiente kappa de 0,956 e 0,699, respectivamente. Esses resultados permitem afirmar que o teste de cELISA-PR1 apresentou performance equivalente ao cELISA-USA, podendo também ser utilizado em estudos epidemiológicos, programas de controle e movimentação internacional de animais, enquanto a IFI, com os resultados menos precisos apresentados, não deve ser utilizada em situações que requerem maior rigor no diagnóstico.Palavras-chave: Anaplasma marginale, ELISA, IFI, proteínas, bovinos. AbstractA competitive enzyme-linked immunosorbent test using the PR1 recombinant major surface protein 5 (rMSP5-PR1-ELISA)of Anaplasma marginale was standardized and validated using sera from anaplasmosis free and endemic regions. The sequencing of the msp5 gene of PR1 isolate showed 98% of identity with the Florida and Saint Maries isolates, 97% with Brazil (Pernambuco) and Havana isolates; and 91% with A. centrale. The cELISA-PR1 test was compared to IFI and cELISA-USA. For the standardization and validation of the cELISA-PR1, 380 bovine sera were used, whereas 245 truly positives and 135 truly negatives sera tested by the cELISA-USA. In the standardization of the cELISA-PR1 135 negative and 148 positive bovine sera were used. The cELISA-PR1 and IFI tests showed 100 and 99.3% specificity, 100 and 98%, sensibility, and a kappa coefficient of 0.993 and 0.978, respectively. For test validation, 245 bovine sera from an anaplasmosis endemic area were analyzed by the cELISA-PR1 and IFI, which showed 96.7 and 69.1% specificity, 98.9 e 96.3% sensibility and kappa coefficient of 0.956 and 0.699, respectively. These results indicate that the cELISA-PR1, likewise the cELISA-USA, could sensitively and specifically detect cattle naturally infected with A. marginale and would be recommended for epidemiological studies, eradications program, and regulation of in...

show abstract

Epidemiology of bovine babesiosis and anaplasmosis in Zambia

Cited by 37 publications

References 11 publications

The current status of major tick borne diseases in Zambia

The current status of major tick borne diseases in Zambia

Soroprevalência de Anaplasma marginale em bovinos da região Centro-Sul do estado do Paraná, Brasil, por um teste imunoenzimático competitivo utilizando proteína recombinante MSP5-PR1

Clonagem, expressão, caracterizaÇão molecular da proteína de superfície MSP5 da amostra PR1 de Anaplasma marginale e sua aplicaÇão em um teste de ELISA por competiÇão

Contact Info

Product

Resources

About