Epidemiology of symptomatic human rotaviruses in Bangalore and Mysore, India, from 1988 to 1994 as determined by electropherotype, subgroup and serotype analysis

Aijaz, Saima; Gowda, Krishne; Jagannath, H. V.; Reddy, Raghunath; Maiya, P. P.; Ward, Richard L.; Greenberg, Harry B.; Raju, Mohankumar; Babu, A. Giridhar; Rao, D. Narayana

doi:10.1007/bf01718329

Cited by 42 publications

(47 citation statements)

References 49 publications

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“…In the present study, 36.36% (12/33) rotavirus infection was detected among diarrheic children in central India (Table 1). Similar prevalence (36%) in human children was reported in Bangalore 16 , whereas in a later study 21.6% prevalence was reported in Bangalore and only 11.2% in Mysore 5 . Lower prevalence (22.55%) of human rotavirus was also recorded among children with acute diarrhea in Chennai 11 .…”

Section: Resultssupporting

confidence: 79%

“…Epidemiological and molecular studies in many countries show complex patterns of change year to year in the serotypes and electropherotypes that cause diarrhea in children from the same geographical areas 3 . Although relatively few studies have examined the molecular epidemiology of human rotaviruses in India 4,5 report is still lacking from central India. Present study investigated the cause of gastroenteritis in hospitalized diarrheic children in this region of India and existence of genomic diversity amongst the circulating strains.…”

Section: Introductionmentioning

confidence: 99%

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Detection of human rotavirus in hospitalized diarrheic children in central India

et al. 2007

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During the present study, group A human rotaviruses were detected among diarrheic children using polyacrylamide gel electrophoresis (PAGE) technique, with a typical RNA migration pattern of 4:2:3:2, suggestive of group A rotavirus. During the study, a total of 46 fecal samples collected from hospitalized children with acute diarrhea as well as children inhabiting nearby animal farms with history of presence of animal rotaviruses on the farms were processed for detection of human rotavirus. Out of 33 diarrheic children, 12 showed presence of rotavirus infection (36.36%), however, none of the children from animal farm areas showed presence of rotavirus. Female children were more susceptible to rotavirus infection (46.15%) than males (30%). Majority of the cases of rotavirus gastroenteritis belonged up to one year of the age, with an incidence of 40.91%. RNA profi le of rotaviruses suggested circulation of 5 different electropherotypes in this geographical locale of the country, indicating existence of genomic diversity among human rotaviruses. Majority of the isolates were of long pattern (66.67%), whereas short pattern was detected only in one third of the viruses. This preliminary study emphasizes for further detailed studies on the molecular characterization of rotaviruses circulating in this part of country and their relationship with other human rotavirus strains and animal strains in the country.

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Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Detection of human rotavirus in hospitalized diarrheic children in central India

et al. 2007

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“…A distribuição dos diferentes sorotipos G no País e no Estado é similar ao reportado a nível mundial 1 .…”

Section: Discussionunclassified

“…Estas correlações eletroforéticas e antigênicas têm-se perdido com o aparecimento de novas cepas de padrão curto que não reagem de maneira específica com os monoclonais de sorotipo G2 1 .…”

Section: Discussionunclassified

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Identificação de rotavirus associado ao sorotipo G2 em Yucatan, México

Gonzales-loza

Polanco-Marín

Puerto-Solı́s

2000

Rev. Soc. Bras. Med. Trop.

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Resumo Neste estudo reporta-se a identificação de rotavirus sorotipo G2 proveniente de amostras fecais de crianças com gastroenterite, da Cidade de Mérida, Yucatán, México. O diagnóstico virológico foi feito através da eletroforese em gel de poliacrilamidia e ensaio imunoenzimático. Das 149 amostras estudadas, 25 (16,7%) foram positivas para rotavirus do grupo A; dessas 23 (92%) foram sorotipo G2, subgrupo I e padrão eletroforético curto e 2 (8%) subgrupo II e padrão eletroforético longo, porém, não foi possível determinar o sorotipo G. Desde 1985, até hoje, foi a única vez que o sorotipo G2 foi identificado, em mais de 90% das amostras analisadas. Acreditase que, provavelmente, nos últimos anos tem acumulado na população, um grupo de pessoas suscetíveis ao sorotipo de RV, e num futuro poderia ter um surto importante de gastroenterite, associado ao sorotipo G2 de rotavirus. Palavras-chaves: Rotavirus. Sorotipo G2. Gastroenterite. AbstractIn the present study, rotavirus G2 serotype was identified from fecal samples of children with gastroenteritis from the city of Merida, Yucatan, Mexico. Virological diagnosis of disease was performed using polycrylamide gel electrophoresis and immunoenzymatic assay. Out of 149 analyzed samples 25 (16.7%) gave positive reaction to rotavirus groups A, of these 23 (92%) were identified as serotype g2, subgroup i and electrophoretic short pattern, whereas 2 (8%) were identified as subgroups II and electrophoretic long pattern, however, the G serotype was not possible to determine. Rotavirus G serotype has not been detected in more than 90% of samples since 1985. This indicates that the number of people susceptible to G2 serotype within the population has increased over recent years, which perhaps indicates that an important outbreak of acute infectious diarrhea caused by the rotavirus G2 serotype may be forthcoming. Key-words: Rotavirus. G serotype. Gastroenteritis. O grupo A de rotavirus (RV) é a causa mais comum de gastroenterite aguda em crianças de todo o mundo. Nos países em desenvolvimento estima-se que, anualmente, ocorra mais de 870.000 mortes, em crianças menores de 5 anos 10 24 .Os rotavirus contêm um genoma de 11 segmentos de RNA de dupla cadeia, envolvidos em três camadas de proteínas 5 12 . Os pesos moleculares dos 11 segmentos têm servido para sua classificação, de acordo com seu padrão de migração no gel de poliacrilamida. Deste modo, temos que os RV se classificam em padrões curtos e longos, dependendo da migração do segmento 10 1 16 .A camada externa destes vírus está formada por duas proteínas (VP4 e VP7) que possuem epítopos que induzem à formação de anticorpos neutralizantes 2 6 .

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Epidemiological study of the G serotype distribution of group A rotaviruses in Kenya from 1991 to 1994

et al. 1999

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An epidemiological study on the G serotype distribution of group A rotaviruses (GARV) isolated in Kenya was carried out in one urban hospital in Nairobi and in two rural hospitals in Nanyuki and Kitui to clarify the prevalent G serotypes before future introduction of the ready licensed rotavirus vaccine in Kenya. A total of 1,431 stool specimens were collected from children, who were mainly outpatients, aged from 0 to 6 years old with acute gastroenteritis from August 1991 to July 1994. Samples positive for GARV by conventional ELISA were then analyzed by subgrouping and serotyping ELISA and by PAGE. To ascertain the G serotypes of viruses in samples that were unable to be typed by serotyping ELISA, polymerase chain reaction was also attempted. The prevalence of GARV was 28.4% in the urban hospital, 22.5% in Nanyuki, and 13.7% in Kitui. Among rotavirus-positive samples, subgroup II rotaviruses were detected in 63.1%, and subgroup I rotaviruses were 25.9%. Serotype G4 was most prevalent, accounting for 41.6% followed by 23.3% of serotype G1, 17.0% of serotype G2, and serotype G3 was rarely isolated. Seven strains of serotype G8/P1B rotavirus was detected for the first time in Kenya by RT-PCR. Eleven specimens with an unusual composition of subgroup, serotype, and electropherotype were atypical GARV in which the P-serotype was P1A, P1B, or P2. Although uncommon GARV serotype G8/P1B and atypical GARV were detected, the four major GARV serotypes, G1 through G4, should be targeted at this moment for vaccination to control this diarrheal disease in Kenya. Continuous monitoring of the G- and P-serotype distribution of GARV should provide important information about the impact of rotavirus vaccination in Kenya.

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Epidemiology of symptomatic human rotaviruses in Bangalore and Mysore, India, from 1988 to 1994 as determined by electropherotype, subgroup and serotype analysis

Cited by 42 publications

References 49 publications

Detection of human rotavirus in hospitalized diarrheic children in central India

Detection of human rotavirus in hospitalized diarrheic children in central India

Identificação de rotavirus associado ao sorotipo G2 em Yucatan, México

Epidemiological study of the G serotype distribution of group A rotaviruses in Kenya from 1991 to 1994

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