Epidermal changes caused by chronic low-dose UV irradiation induce wrinkle formation in hairless mouse

Kambayashi, Hiroaki; Yamashita, Masahiro; Odake, Yuki; Takada, Koji; Funasaka, Yoko; Ichihashi, Masamitsu

doi:10.1016/s0923-1811(01)00113-x

Cited by 65 publications

(60 citation statements)

References 15 publications

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“…Previous studies in animal models indicate the cause effect relationship between repetitive UV irradiation and an increase of keratins K1, K5, and K10, with no changes in the collagen fibers and elastic fibers, resulting in loss of skin elasticity and the formation of skin wrinkles. 12,13 Conversely, in our study, repeated UVB irradiation stimulated a stable expression of keratins K6 and its partners K16 and K17, but not K1, K5, and K10. Keratins K6, K16, and/or K17, which are not usually expressed in the innate state of normal epidermis, have been reported to initially induce in the suprabasal layers as early as 6 hours after injury or during the first 24 to 48 hours of UVB irradiation.…”

Section: Discussioncontrasting

confidence: 60%

“…[1][2][3] Skin photodamage is physiologically correlated with several alterations including the increase and disorganization of elastic fibers and the reduction of collagens in the dermal extracellular matrix 4 -11 and the increase of keratin contents and the deterioration of keratin intermediate filaments (KIFs) in the epidermis. [12][13][14] Elastic fibers are age-dependent-decreasing dermal extracellular matrix that respond primarily to the fibrous mechanism controlling cutaneous elasticity. 4,5,15,16 The accumulation of dystrophic elastotic material in the reticular dermis, referred to as solar elastosis, is commonly observed in photoaged skin.…”

mentioning

confidence: 99%

“…38 -41 In hairless mice, a repeated low-dose UV exposure has been reported to deteriorate KIFs and induce the expression of epithelial keratins (K1, K5, and K10), resulting in the loss of skin elasticity and eventually initiating wrinkled skin, consistent with previous reports demonstrating no causal relationship between wrinkle formation and dermal changes. [12][13][14]42,43 Many studies of skin photoaging have been conducted using animal models and human skin substitute. The results may be misleading because of inferior architecture, such as the relative thin epidermal layer and compromised barrier function.…”

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confidence: 99%

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Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Hachiya

Sriwiriyanont

Fujimura

et al. 2009

The American Journal of Pathology

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UVB irradiation has been reported to induce photoaging and suppress systemic immune function that could lead to photocarcinogenesis. However, because of the paucity of an UVB-induced photodamaged skin model, precise and temporal mechanism(s) underlying the deleterious effects of long-term UVB exposure on human skin have yet to be delineated. In this study, we established a model using human skin xenografted onto severe combined immunodeficient mice, which were subsequently challenged by repeated UVB irradiation for 6 weeks. Three-dimensional optical image analysis of skin replicas and noninvasive biophysical measurements illustrated a significant increase in skin surface roughness, similar to premature photoaging, and a significant loss of skin elasticity after long-term UVB exposure. Resembling authentically aged skin, UVB-exposed samples exhibited significant increases in epithelial keratins (K6, K16, K17), elastins, and matrix metalloproteinases (MMP-1, MMP-9, MMP-12) as well as degradation of collagens (I, IV, VII). The UVB-induced deterioration of fibrous keratin intermediate filaments was also observed in the stratum corneum. Additionally, similarities in gene expression patterns between our model and chronologically aged skin substantiated the plausible relationship between photodamage and chronological age. Furthermore , severe skin photodamage was observed when neutralizing antibodies against TIMP-1 , an endogenous inhibitor of MMPs , were administered during the UVB exposure regimen. Taken together , these findings suggest that our skin xenograft model recapitulates premature photoaged skin and provides a comprehensive tool with which to assess the deleterious effects of UVB irradiation.

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Section: Discussioncontrasting

confidence: 60%

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confidence: 99%

mentioning

confidence: 99%

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Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Hachiya

Sriwiriyanont

Fujimura

et al. 2009

The American Journal of Pathology

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“…This makes it possible to show an extrinsic influence on the skin such as UV radiation [33, 34]. UVA induces epidermal pigmentation [33].…”

Section: Discussionmentioning

confidence: 99%

Noninvasive Determination of Epidermal and Stratum Corneum Thickness in vivo Using Two-Photon Microscopy and Optical Coherence Tomography: Impact of Body Area, Age, and Gender

Czekalla

Schönborn

Lademann

et al. 2019

Skin Pharmacol Physiol

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Background: High-resolution images of the epidermis are important to understand the transdermal penetration and changes in epidermal components. Both ex vivo and in vivo technologies are available to picture the epidermal thickness (ET). So far, the illustration of the stratum corneum (SC) has not been possible without artifacts. Objective: Precision in vivo measurement of the ET and SC, duly considering the impact of location on the body, age, and gender. Methods: In this pilot study on 20 skin-healthy subjects aged 18–66 years, the ET was imaged by two-photon microscopy (2PM) and optical coherence tomography (OCT), and the SC by using 2PM at five different body sites. Results: On solar-exposed body areas, both the epidermis and SC are thicker compared to solar-protected areas (p < 0.05), the epidermis at the gluteal region being the thickest (p < 0.05). The ET decreases with age (p < 0.05). Males show a thicker epidermis than females (p < 0.05). Conclusion: 2PM provides a noninvasive method for imaging the epidermis and especially the SC in vivo and is optimally suited for the application of histological criteria.

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“…As the permeability barrier mainly exists in the SC and the SC integrity in the present study was determined by measuring TEWL following each SC removal with Dsquame, SC thickness could influence TEWL after each SC removal. Studies have demonstrated that repeated UVB radiation increases both epidermal and SC thickness [52][53][54][55] . Therefore, more SC layers were left with higher doses of sun exposure following the same number of D-squame application in comparison with a thinner SC in the control group.…”

Section: Discussionmentioning

confidence: 99%

Sun-Induced Changes in Stratum Corneum Function Are Gender and Dose Dependent in a Chinese Population

Liu

Fluhr

Shu-min

et al. 2010

Skin Pharmacol Physiol

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Previous studies have demonstrated that UVB radiation changes the epidermal permeability barrier and stratum corneum (SC) hydration. It is well known that sun exposure causes erythema, sunburn and melanoma. However, whether daily sun exposure alters SC integrity and epidermal permeability barrier function is largely unknown, especially in Chinese subjects. In the present study, we assess the SC integrity, SC hydration and epidermal permeability barrier function following various doses of sun exposure. A total of 258 subjects (124 males and 134 females) aged 18–50 years were enrolled. A multifunctional skin physiology monitor (Courage & Khazaka MPA5) was used to measure SC hydration and transepidermal water loss (TEWL) on the forearms. In males, basal TEWL was higher with higher doses of sun exposure than with lower doses and control, whereas in females, basal TEWL was higher with lower doses of sun exposure than with higher doses and control. In the group with higher doses of sun exposure, TEWL in females was significantly lower than that in males. The barrier recovery was faster in females than in males in both control and lower-dose groups. In both males and females, barrier recovery was delayed with higher doses of sun exposure. In males, sun exposure did not alter SC hydration, while in females SC hydration was lower with lower doses of sun exposure as compared with control and higher doses of sun exposure. These results demonstrated that sun-induced changes in SC function and SC hydration vary with gender and the extent of sun exposure.

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Epidermal changes caused by chronic low-dose UV irradiation induce wrinkle formation in hairless mouse

Cited by 65 publications

References 15 publications

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Mechanistic Effects of Long-Term Ultraviolet B Irradiation Induce Epidermal and Dermal Changes in Human Skin Xenografts

Noninvasive Determination of Epidermal and Stratum Corneum Thickness in vivo Using Two-Photon Microscopy and Optical Coherence Tomography: Impact of Body Area, Age, and Gender

Sun-Induced Changes in Stratum Corneum Function Are Gender and Dose Dependent in a Chinese Population

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