Epidermal growth factor in human and bovine milk

Iacopetta, BJ; Grieu, Fabienne; Horisberger, M.; Sunahara, GI

doi:10.1111/j.1651-2227.1992.tb12227.x

Cited by 50 publications

(33 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pakkanen and Aalto (1997) reported that the concentrations of IGF-I and IGF-II in colostrum range from 50 to 2000 μg.L −1 and 200-600 μg.L −1 , respectively, whereas mature milk contains <10 μg.L −1 of each. Yagi et al (1986) found that the concentration of EGF in colostrum and milk was 324 and 155 μg.L −1 , respectively; however, Iacopetta et al (1992) reported the concentration of EGF in colostrum and milk to be 4-8 and <2 μg.L −1 , respectively. It should be noted that reported levels of growth factors vary significantly depending on the method of quantitation used.…”

Section: Growth Factorsmentioning

confidence: 99%

“…The main growth factors in colostrum and milk are epidermal growth factor (EGF) (Yagi et al 1986;Iacopetta et al 1992), betacellulin (BTC) (Bastian et al 2001), insulin-like growth factor I (IGF-I) (Collier et al 1991), IGF-II (Schams 1994), transforming growth factor-β1 (TGF-β1) (Ginjala and Pakkanen 1998), TGF-β2 (Cox and Burk 1991), fibroblast growth factor 1 and 2 (FGF1 and FGF2) and platelet-derived growth factor (PGDF) (Belford et al 1997).…”

Section: Growth Factorsmentioning

confidence: 99%

See 1 more Smart Citation

Composition and properties of bovine colostrum: a review

McGrath

Fox

McSweeney

et al. 2015

Dairy Sci. & Technol.

312

263

View full text Add to dashboard Cite

Colostrum is the initial milk secreted by mammals following parturition, the composition and physicochemical properties of which are highly dynamic and variable. The composition and physicochemical properties of colostrum during the initial postpartum period has not been systematically reviewed for many years, although the topic remains of interest both to milk producers and processors. In this article, the current understanding of the composition of colostrum, i.e. carbohydrates, proteins, growth factors, enzymes, enzyme inhibitors, nucleotides and nucleosides, cytokines, fats, vitamins and minerals, is reviewed. In addition, the physicochemical properties, i.e. pH and buffering capacity, colour, density and specific gravity, osmotic pressure, somatic cell count, properties of casein micelles, ethanol stability and rennet coagulation properties are discussed, as well as the effects of heat-treating colostrum.

show abstract

Section: Growth Factorsmentioning

confidence: 99%

Section: Growth Factorsmentioning

confidence: 99%

Composition and properties of bovine colostrum: a review

McGrath

Fox

McSweeney

et al. 2015

Dairy Sci. & Technol.

312

263

View full text Add to dashboard Cite

show abstract

“…Amniotic fluid contains significant concentrations of EGF that gradually increase during pregnancy, with the highest level achieved at the end of the normal gestation period (36). In contrast to human milk, EGF is absent in commercially available infant formulas (37,38). Clinical studies have shown reduced serum and salivary EGF levels in neonates with NEC when compared with healthy babies (39,40).…”

mentioning

confidence: 99%

Increased Epidermal Growth Factor Levels in Human Milk of Mothers with Extremely Premature Infants

et al. 2003

View full text Add to dashboard Cite

Maternal milk is the major source of nutrients and growthpromoting substances in the first weeks of life for the majority of neonates. Epidermal growth factor (EGF) and transforming growth factor-␣ (TGF-␣) are trophic peptides present in human milk with significant healing effects on injured gastrointestinal mucosa. Decreasing gestational age of neonates is associated with higher risk of developing gastrointestinal disorders, and human milk provides better protection against these diseases compared with formula. The aim of this study was to evaluate the concentrations of EGF and TGF-␣ in human milk collected from mothers with infants born: extremely preterm, preterm, and full term. Milk samples were collected at the end of first, second, and fourth week postpartum from each mother of infants born in one of the three gestational age groups: extremely preterm (23-27 wk, n ϭ 16), preterm (32-36 wk, n ϭ 16), and full term (38 -42 wk, n ϭ 15). Milk concentrations of EGF and TGF-␣ were quantified with a homologous RIA in the milk aqueous fraction. Concentrations of EGF in human milk from the extremely preterm group (23-27 wk) were significantly higher compared with values from the preterm and full-term groups throughout the first month of lactation. A similar pattern was observed with human milk TGF-␣; however, milk TGF-␣ levels were lower than EGF.In conclusion, we have found higher concentrations of EGF and TGF-␣ in human milk of mothers with extremely preterm babies. These data may indicate the potential importance of milk-borne EGF and TGF-␣ for the development of extremely premature infants. (Pediatr Res 54: 15-19, 2003) Abbreviations EGF, epidermal growth factor EGF-R, epidermal growth factor receptor EPT, extremely preterm FT, full term PT, preterm NEC, necrotizing enterocolitis TGF-␣, transforming growth factor-␣ Human milk is a unique and well-balanced source of nutrition for the newborn. Milk contains not only major nutrients (proteins, carbohydrates, and lipids) but also a variety of components such as minerals, vitamins, enzymes, hormones, growth factors, and immunoglobulins important for growth and healthy development during the neonatal period (1-3). As advances in neonatal care have significantly increased, the survival of extremely prematurely born neonates have increased (4). Yet these infants have the highest incidence of morbid events, such as NEC (5). Questions have arisen about the most suitable and relevant nutritional support for these immature infants (6, 7).Within the last three decades, a large number of biologically active peptides have been identified in human milk (8 -10), and the list is continuously growing (11-13). Among these, EGF is one of the major peptide growth factors present both in colostrum and human milk (14 -17). Human milk EGF levels are highest in the first days after parturition and then gradually decrease during the first 2 wk of lactation (17). Another structural homolog of EGF, TGF-␣ is also present in human colostrum and milk (15, 18), but at much lower concentrations ...

show abstract

“…In human milk, EGF has been identified as a major growthpromoting factor [38], whereas there seems to be very little, if any, EGF in bovine milk [29]. Instead, the major growthpromoting factors identified in bovine milk seem to be PDGF [27] and IGF-I and IGF-II [28].…”

Section: Discussionmentioning

confidence: 99%

“…In bovine milk and colostrum, cell growth stimulatory activity has been attributed to a number of peptide growth factors including platelet-derived growth factor (PDGF) [27] and insulin-like growth factor I and II (IGF-I and IGF-II) [28]. EGF, although abundant in human milk, has not been unequivocally detected in bovine milk or colostrum [29].…”

Section: Introductionmentioning

confidence: 99%

Identification of betacellulin as a major peptide growth factor in milk: purification, characterization and molecular cloning of bovine betacellulin

Dunbar

Priebe

Belford

et al. 1999

Biochemical Journal

View full text Add to dashboard Cite

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family of peptide growth factors, was purified from a growth-factor-enriched whey fraction of bovine milk by a combination of ion-exchange chromatography, gel-filtration chromatography, affinity chromatography and reverse-phase HPLC. Bovine BTC (bBTC) had an apparent molecular mass of 21-22 kDa on SDS/PAGE and exists in a glycosylated form. The cDNA encoding bBTC was obtained by a combination of 5ʹ and 3ʹ rapid amplification of cDNA ends (‘RACE’). The primary translation product consists of 178 amino acid residues containing a putative signal sequence, a transmembrane domain, the mature BTC domain and a cytoplasmic domain containing a highly hydrophilic Arg-Lys-rich region similar to that of mouse BTC and human BTC. The amino acid sequence of the bBTC precursor was 88% identical with human BTC and 79% identical with mouse BTC. The bBTC gene was found to be expressed in a wide range of tissues, including the mammary gland. The identification of BTC in milk raises the possibility that it has a major role in the growth and development of the neonatal gastrointestinal tract.

show abstract

Epidermal growth factor in human and bovine milk

Cited by 50 publications

References 19 publications

Composition and properties of bovine colostrum: a review

Composition and properties of bovine colostrum: a review

Increased Epidermal Growth Factor Levels in Human Milk of Mothers with Extremely Premature Infants

Identification of betacellulin as a major peptide growth factor in milk: purification, characterization and molecular cloning of bovine betacellulin

Contact Info

Product

Resources

About