Epidermal growth factor receptor (EGFR) gene copy number detection in non‐small‐cell lung cancer; a comparison of fluorescence in situ hybridization and chromogenic in situ hybridization

Abstract

CISH is a suitable alternative strategy to FISH in determining EGFR gene copy number in NSCLC patients.

Reliability of chromogenic in situ hybridization for epidermal growth factor receptor gene copy number detection in non-small-cell lung carcinomas: A comparison with fluorescence in situ hybridization study

“…Another problem is that it is difficult to detect the overall morphology and tumor heterogeneity. Recently, chromogenic in situ hybridization (CISH) for the assessment of EGFR status in NSCLC has been tested as an emerging alternative method to FISH in a few studies [5,6]. The main difference between FISH and CISH is how the probe signals are detected.…”

EGFR fluorescence in situ hybridization-positive lung adenocarcinoma: incidence of coexisting KRAS and BRAF mutations

“…Although there is a growing evidence that DNA mutational analysis is the most reliable predictor of the tumor response to EGFR TKI, there is still ongoing discussion about the role of gene copy number (GCN) analysis determined by fluorescence in situ hybridization (FISH) or chromogenic in situ hybridization (CISH) [7][8][9][10][11][12][13]. Important limitation of CISH is its inability to assess for polysomy simultaneously with evaluation of EGFR GCN.…”

Combined assessment of EGFR pathway-related molecular markers and prognosis of NSCLC patients

“…Fluorescent in situ hybridisation (FISH) was performed only on frozen sections, as this analysis provides poorer results on paraffin-embedded tissue (Gallegos Ruiz et al, 2007b). Frozen sections of 4 mm thickness were fixed with methanol/acetic acid (3 : 1) and pretreated by digestion with 0.01% pepsin/0.2N HCl at 37 1C for 2 min, and incubated for 10 min in 50 mM MgCl2/PBS followed by 10 min in 50 mM MgCl 2 /3.7% formaldehyde/PBS.…”