Epidermal growth factor receptor genes are overexpressed within the periprosthetic soft‐tissue around percutaneous devices: A pilot study

Jeyapalina, Sujee; Colombo, John S.; Beck, James P.; Agarwal, Jayant P.; Schmidt, Linda; Bachus, Kent N.

doi:10.1002/jbm.b.34409

Cited by 2 publications

(2 citation statements)

References 56 publications

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“…Previous studies have shown that FGF-10, EGF, EGFr, and TGF-α are all diferentially expressed within the GT when compared to the healthy skin (i.e., uninjured/ unmodeled dermal tissue) [36][37][38][39][40]. To better understand the progression of healing around the implants, mRNAs were quantifed using qPCR.…”

Section: Discussionmentioning

confidence: 99%

Fluorapatite-Coated Percutaneous Devices Promote Wound Healing and Limit Epithelial Downgrowth at the Skin-Device Interface

Steyl

Beck

Agarwal

et al. 2023

Journal of Tissue Engineering and Regenerative Medicine

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A percutaneous osseointegrated device becomes deeply ingrown by endosteal bone and traverses the overlying soft tissues of the residual limb, providing a direct link to the bone-anchored artificial limb. Continuous wound healing around these devices can result in the formation of sinus tracts as “down-growing” epithelial cells are unable to recognize and adhere to the “nonbiological” implant surface. Such sinus tracts provide paths for bacterial colonization and deep infection. In order to limit adverse outcomes and provide a robust seal, it was hypothesized that by coating the titanium surface of the percutaneous post with the mineral component of dental enamel, down-growing epidermal cells might recognize the coating as “biological” and adhere to this nonliving surface. To test this hypothesis, sintered partially and fully fluoridated hydroxyapatite (HA) was chosen as coatings. Using an established surgical protocol, fluorapatite (FA), hydroxyfluorapatite (FHA), HA-coated percutaneous posts, and titanium controls were surgically placed under the dorsal skin in 20 CD hairless rats. The animals were sacrificed at four weeks, and implants and surrounding tissues were harvested and subjected to further analyses. Downgrowth and granulation tissue area data showed statistically significant reductions around the FA-coated devices. Moreover, compared to the control group, the FA- and HA-coated groups showed downregulation of mRNA for EGFr, EGF, and FGF-10. Interestingly, the FA-coated group had upregulation of TGF-α. These data suggest that FA could become an ideal coating material for preventing downgrowth, assuming the long-term stability of these coated surfaces can be verified in a clinically relevant animal model.

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Section: Discussionmentioning

confidence: 99%

Fluorapatite-Coated Percutaneous Devices Promote Wound Healing and Limit Epithelial Downgrowth at the Skin-Device Interface

Steyl

Beck

Agarwal

et al. 2023

Journal of Tissue Engineering and Regenerative Medicine

Self Cite

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show abstract

“…This may relate to integrin subunit β4 and its participation in EGF-stimulated chemotaxis [ 246 ] or maintenance of epithelial integrity [ 294 ]. The potential role for EGF in percutaneous device/epithelial integrity is supported by a recent report showing EGF and both up- and down-stream genes were highly upregulated in soft tissue immediately surrounding percutaneous implants placed in the backs of rats [ 295 ]. EGF thus may mediate wound healing response to percutaneous device placement, perhaps by controlling HD disassembly as discussed.…”

Section: Important Biomolecules In Junctional Epithelium Homeostatis ...mentioning

confidence: 99%

Junctional epithelium and hemidesmosomes: Tape and rivets for solving the “percutaneous device dilemma” in dental and other permanent implants

Fischer

Aparicio

2022

Bioactive Materials

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Epidermal growth factor receptor genes are overexpressed within the periprosthetic soft‐tissue around percutaneous devices: A pilot study

Abstract: This is the author manuscript accepted for publication and has undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as

Cited by 2 publications

References 56 publications

Fluorapatite-Coated Percutaneous Devices Promote Wound Healing and Limit Epithelial Downgrowth at the Skin-Device Interface

Fluorapatite-Coated Percutaneous Devices Promote Wound Healing and Limit Epithelial Downgrowth at the Skin-Device Interface

Junctional epithelium and hemidesmosomes: Tape and rivets for solving the “percutaneous device dilemma” in dental and other permanent implants

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