Epidermal Growth Factor Variations in Amniotic Membrane Used for<i>Ex Vivo</i>Tissue Constructs

Gicquel, Jj; Dua, Harminder S.; Brodie, Andrew; Mohammed, Imran; Suleman, Hanif; Lazutina, Elena; James, David; Hopkinson, Andrew

doi:10.1089/ten.tea.2008.0432

Cited by 62 publications

(48 citation statements)

References 52 publications

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“…The amniotic membrane has similar histological components as the basal membrane of ocular superficial epithelium and can secret cytokines such as alkaline fibroblastic growth factor and epidermal growth factor to promote epithelial adhesion, migration, and induced differentiation (Lee et al, 2006;Deolinda et al, 2007;Gicquel et al, 2009;Wolbank et al, 2009), and is widely used in the ophthalmology clinic. Owing to the removal of epithelia, the immunogenicity and rejection reaction are further decreased; therefore, the decellularized amniotic membrane is an ideal vector to construct engineered transplantation material for the surface of the eye (Koizumi et al, 2007).…”

Section: Tissue-engineering Conjunctiva In Re-constructing Complete Ementioning

confidence: 99%

Conjunctiva reconstruction by induced differentiation of human amniotic epithelial cells

Yang¹,

Yang²,

Cao³

2015

Genet. Mol. Res.

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ABSTRACT. In this study, we aimed to investigate the feasibility of directed differentiation of human amniotic epithelial cells into conjunctival epithelium under specific conditions as well as of constructing tissueengineered conjunctiva for ocular surface reconstruction. Human amniotic epithelial cells were cultured with induced denuded conjunctival matrix and conjunctival homogenate. Immunohistochemistry of cytokeratin-4, cytokeratin-13, and muc5ac as well as PAS staining were performed. The concentration of muc5ac at different times was measured using ELISA. The differentiated cells with quantum dots were transferred onto a denuded amniotic membrane to establish tissue-engineered conjunctiva and transplanted into a rabbit model with a conjunctival defect. After induction of human amniotic epithelial cells, differentiated cells showed conjunctival epithelium phenotype, while trace amounts of mu5ac in the culture medium measured by ELISA increased gradually within 1 to 7 days. Successfully tissue-engineered conjunctiva had similar structure as normal conjunctiva and was transplanted into a rabbit model with conjunctiva defect. After 2 weeks post-surgery, conjunctiva grafts 13824 S.P.Yang et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 13823-13834 (2015) survived and were integrated. Immunohistochemistry showed conjunctival epithelium phenotype, positive cells were found in PAS staining. Thus, human amniotic epithelial cells could differentiate into conjunctival epithelium-like cells and goblet cells with partially physiological function, and we successfully restored ocular surface integrity in the rabbit model using tissue-engineered conjunctiva.

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Section: Tissue-engineering Conjunctiva In Re-constructing Complete Ementioning

confidence: 99%

Conjunctiva reconstruction by induced differentiation of human amniotic epithelial cells

Yang¹,

Yang²,

Cao³

2015

Genet. Mol. Res.

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“…Written informed consent from the donor (participant age range [25][26][27][28][29][30][31][32][33][34][35][36][37] or the next of kin was obtained for sample use in this research project and prepared according to previously published methodology [45]. Patients with a history of antenatal problems e.g.…”

Section: Tissue Procurement and Preparationmentioning

confidence: 99%

“…Wolbank et al [25] has additionally shown that there are significantly lower levels of angiogenic factors in cryopreserved AM compared to fresh but they did not assess lyophilised AM. A number of studies have reported extensive depletion of soluble factors, presumed to be beneficial, from cryopreserved AM [26][27][28]. While frozen preparations of AM account for the majority of procedures, dried preparations have gained popularity as substrates for epithelial growth during ocular surface reconstruction [29,30], to treat corneal perforations and leaks [31] and pterygium [32].…”

Section: Introductionmentioning

confidence: 99%

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Allen¹,

Clare²,

Stewart³

et al. 2013

Acta Ophthalmologica

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Purpose: Dried amniotic membrane (AM) can be a useful therapeutic adjunct in ophthalmic surgery and possesses logistical advantages over cryopreserved AM. Differences in preservation techniques can significantly influence the biochemical composition and physical properties of AM, potentially affecting clinical efficacy. This study was established to investigate the biochemical and structural effects of drying AM in the absence and presence of saccharide lyoprotectants and its biocompatibility compared to cryopreserved material.Methods: AM was cryopreserved or dried with and without pre-treatment with trehalose or raffinose and the antioxidant epigallocatechin (EGCG). Structural and visual comparisons were assessed using electron microscopy. Localisation, expression and release of AM biological factors were determined using immunoassays and immunofluorescence. The biocompatibility of the AM preparations co-cultured with corneal epithelial cell (CEC) or keratocyte monolayers were assessed using cell proliferation, cytotoxicity, apoptosis and migration assays.Results: Drying devitalised AM epithelium, but less than cryopreservation and cellular damage was reduced in dried AM pre-treated with trehalose or raffinose. Dried AM alone, and with trehalose or raffinose showed greater factor retention efficiencies and bioavailability compared to cryopreserved AM and demonstrated a more sustained biochemical factor time release in vitro. Cellular health assays showed that dried AM with trehalose or raffinose are compatible and superior substrates compared to cryopreserved AM for primary CEC expansion, with increased proliferation and reduced LDH and caspase-3 levels. This concept was supported by improved wound healing in an immortalised human CEC line (hiCEC) cocultured with dried and trehalose or raffinose membranes, compared to cryopreserved and fresh AM.Conclusions: Our modified preservation process and our resultant optimised dried AM has enhanced structural properties and biochemical stability and is a superior substrate to conventional cryopreserved AM. In addition this product is stable and easily transportable allowing it to be globally wide reaching for use in clinical and military sectors.

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“…It is also rich in growth factors such as transforming growth factor β2 (TGF β2), transforming growth factor β1(TGF β1), hepatocyte growth factor (HGF) and epithelial growth factor (EGF). Their combined action with other cytokines is supposed to stimulate epithelialisation and inhibit fibrosis 14 17. The AM can be used as a patch, epithelial side down, for a maximum concentration of growth factors in contact with the remaining LSC 14 17.…”

mentioning

confidence: 99%

“…Their combined action with other cytokines is supposed to stimulate epithelialisation and inhibit fibrosis 14 17. The AM can be used as a patch, epithelial side down, for a maximum concentration of growth factors in contact with the remaining LSC 14 17. The AM will also act as a barrier against the efflux of immune cells, by tempering the immune response and displaying antiangiogenic properties 14.…”

mentioning

confidence: 99%

Management of ocular surface chemical burns

Gicquel

2010

British Journal of Ophthalmology

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Epidermal Growth Factor Variations in Amniotic Membrane Used forEx VivoTissue Constructs

Cited by 62 publications

References 52 publications

Conjunctiva reconstruction by induced differentiation of human amniotic epithelial cells

Conjunctiva reconstruction by induced differentiation of human amniotic epithelial cells

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Management of ocular surface chemical burns

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