Epidermal Keratinocyte Production of Interferon-γ Immunoreactive Protein and mRNA Is an Early Event in Allergic Contact Dermatitis

Howie, Sarah; Aldridge, R. D.; Mcvittie, E.; Forsey, Rosalyn; Sands, Catherine; Hunter, John

doi:10.1111/1523-1747.ep12348507

Cited by 37 publications

(22 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But in comparison with the Sebutape assay (immunoassay of IL-8 protein; Perkins et al, 2001) for irritation, which has a sensitivity (Hoffrage et al, 2000) of approximately 30%, mRNA biomarkers seem to possess superior potential. Our observation that water occlusion produced increases of our biomarker ratios in some subjects has been reported by others (Grangsjo et al, 1996;Howie et al, 1996;Perkins et al, 2001).…”

Section: Discussionsupporting

confidence: 89%

Use of RT-PCR and DNA Microarrays to Characterize RNA Recovered by Non-Invasive Tape Harvesting of Normal and Inflamed Skin

Wong¹,

Tran²,

Morhenn³

et al. 2004

Journal of Investigative Dermatology

View full text Add to dashboard Cite

We describe a non-invasive approach for recovering RNA from the surface of skin via a simple tape stripping procedure that permits a direct quantitative and qualitative assessment of pathologic and physiologic biomarkers. Using semi-quantitative RT-PCR we show that tape-harvested RNA is comparable in quality and utility to RNA recovered by biopsy. It is likely that tape-harvested RNA is derived from epidermal cells residing close to the surface and includes adnexal structures and present data showing that tape and biopsy likely recover different cell populations. We report the successful amplification of tape-harvested RNA for hybridization to DNA microarrays. These experiments showed no significant gene expression level differences between replicate sites on a subject and minimal differences between a male and female subject. We also compared the array generated RNA profiles between normal and 24 h 1% SLS-occluded skin and observed that SLS treatment resulted in statistically significant changes in the expression levels of more than 1,700 genes. These data establish the utility of tape harvesting as a non-invasive method for capturing RNA from human skin and support the hypothesis that tape harvesting is an efficient method for sampling the epidermis and identifying select differentially regulated epidermal biomarkers.

show abstract

Section: Discussionsupporting

confidence: 89%

Use of RT-PCR and DNA Microarrays to Characterize RNA Recovered by Non-Invasive Tape Harvesting of Normal and Inflamed Skin

Wong¹,

Tran²,

Morhenn³

et al. 2004

Journal of Investigative Dermatology

View full text Add to dashboard Cite

show abstract

“…11,12 Recent studies have indicated that lung epithelial cells may produce a wide range of cytokines. [12][13][14][15][16][17] Such data are similar to those which have been obtained with keratinocytes [18][19][20] and suggest that epithelial cell cytokine production may promote inflammatory responses and influence the development of disease processes.…”

Section: Introductionsupporting

confidence: 63%

Immunoreactive interleukin 4 and interferon-? expression by type II alveolar epithelial cells in interstitial lung disease

Wallace

Howie

1999

J. Pathol.

View full text Add to dashboard Cite

Cryptogenic fibrosing alveolitis (CFA), extrinsic allergic alveolitis (EAA), and sarcoid are all immunologically mediated forms of interstitial lung disease. In contrast to most patients with EAA and sarcoid, patients with CFA show relentless pulmonary fibrosis which is unresponsive to immunosuppressive therapy. Previous studies have indicated a possible role for epithelial‐derived cytokines in the regulation of immunological and fibrotic responses in the lung. This study has examined lung biopsy specimens from patients with CFA, EAA, and sarcoid for immunoreactive interleukin 4 (IL4) and interferon‐γ (INFγ) expression by type II alveolar epithelial cells, as these cytokines have been suggested to have in vitro stimulatory and inhibitory effects on fibroblast function. In addition, mRNA in situ hybridization was performed on the CFA lung biopsies to confirm transcription of these cytokine genes within the cells. The results show that type II epithelial cells in EAA and sarcoid show up‐regulation of immunoreactivity for both IL4 and INF‐γ, but that in CFA only IL4 is detectable. The mRNA in situ hybridization results indicate that this may represent post‐transcriptional regulation of INFγ expression in CFA. These results are consistent with previous observations of a paucity of INFγ and a predominantly type II (Th2‐like) pattern of immune response in patients with CFA and suggest a possible imbalance of pro‐fibrogenic cytokines in the distal lung of patients with this condition, compared with those with EAA or sarcoid. Copyright © 1999 John Wiley & Sons, Ltd.

show abstract

“…More recently, IFN-γ has also been found to be expressed in murine peritoneal macrophages and mast cells, human eosinophils, keratinocytes and primary B cells [1][2][3][4][5]. In vivo, IFN-γ functions as both a proinflammatory effector and a regulator and plays a central part in a diverse array of immunological processes such as inflammatory reactions, cell-mediated immunity and autoimmunity.…”

Section: Introductionmentioning

confidence: 99%

Repression of interferon-γ expression in T cells by Prospero-related Homeobox protein

et al. 2008

View full text Add to dashboard Cite

show abstract

Epidermal Keratinocyte Production of Interferon-γ Immunoreactive Protein and mRNA Is an Early Event in Allergic Contact Dermatitis

Cited by 37 publications

References 22 publications

Use of RT-PCR and DNA Microarrays to Characterize RNA Recovered by Non-Invasive Tape Harvesting of Normal and Inflamed Skin

Use of RT-PCR and DNA Microarrays to Characterize RNA Recovered by Non-Invasive Tape Harvesting of Normal and Inflamed Skin

Immunoreactive interleukin 4 and interferon-? expression by type II alveolar epithelial cells in interstitial lung disease

Repression of interferon-γ expression in T cells by Prospero-related Homeobox protein

Contact Info

Product

Resources

About