Epidermal powder immunization of mice and monkeys with an influenza vaccine

Chen, Dexiang; Endres, Ryan L.; Maa, Yuh‐Fun; Kensil, Charlotte R.; Whitaker-Dowling, Patricia; Trichel, Anita; Youngner, Julius S.; Payne, Lendon G.

doi:10.1016/s0264-410x(03)00175-0

Cited by 49 publications

(44 citation statements)

References 24 publications

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“…20 Infection was confirmed by nasal swabbing performed on days 1 to 7 after infection for measurement of TCID 50 in MDCK cells. 20 In some monkeys, Flt3L (100 g/kg/d) was administered for 10 days before viral infection, and blood and lymphoid tissues were collected 3 and 5 days later.…”

Section: Viral Infection Of Monkeys With Influenza Amentioning

confidence: 99%

Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand

Coates

Barratt‐Boyes

Zhang

et al. 2003

Blood

114

132

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We provide phenotypic and functional evidence of premonocytoid dendritic cells (DCs) and preplasmacytoid DCs in blood and of corresponding DC subsets in secondary lymphoid tissue of rhesus monkeys. Subsets were identified and sorted by 4-color flow cytometry using antihuman monoclonal antibodies cross-reactive with rhesus monkey. To mobilize pre-DC subsets, fms-like tyrosine 3 kinase ligand (Flt3L; 100 g/kg subcutaneously) was administered for 10 days. Presumptive pre-DC subsets were identified within the lineage ؊ (Lin ؊ ) major histocompatibility complex (

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Section: Viral Infection Of Monkeys With Influenza Amentioning

confidence: 99%

Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand

Coates

Barratt‐Boyes

Zhang

et al. 2003

Blood

114

132

View full text Add to dashboard Cite

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“…Skin-based influenza vaccines have utilized approaches including tape stripping (32), epidermal powder immunization (4), and microneedles (MNs) (9). These strategies have used diverse antigens including virus-like particles (VLP) (25), inactivated influenza virus (24), and hemagglutinin (HA) DNA vaccines (1).…”

mentioning

confidence: 99%

Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity

Weldon¹,

Martı́n²,

Zarnitsyn³

et al. 2011

Clin. Vaccine Immunol.

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The emergence of the swine-origin 2009 influenza pandemic illustrates the need for improved vaccine production and delivery strategies. Skin-based immunization represents an attractive alternative to traditional hypodermic needle vaccination routes. Microneedles (MNs) can deliver vaccine to the epidermis and dermis, which are rich in antigen-presenting cells (APC) such as Langerhans cells and dermal dendritic cells. Previous studies using coated or dissolvable microneedles emphasized the use of inactivated influenza virus or virus-like particles as skin-based vaccines. However, most currently available influenza vaccines consist of solubilized viral protein antigens. Here we test the hypothesis that a recombinant subunit influenza vaccine can be delivered to the skin by coated microneedles and can induce protective immunity. We found that mice vaccinated via MN delivery with a stabilized recombinant trimeric soluble hemagglutinin (sHA) derived from A/Aichi/2/68 (H3) virus had significantly higher immune responses than did mice vaccinated with unmodified sHA. These mice were fully protected against a lethal challenge with influenza virus. Analysis of postchallenge lung titers showed that MN-immunized mice had completely cleared the virus from their lungs, in contrast to mice given the same vaccine by a standard subcutaneous route. In addition, we observed a higher ratio of antigen-specific Th1 cells in trimeric sHA-vaccinated mice and a greater mucosal antibody response. Our data therefore demonstrate the improved efficacy of a skin-based recombinant subunit influenza vaccine and emphasize the advantage of this route of vaccination for a protein subunit vaccine.

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“…The use of accelerated particles to deliver drugs was first described in the late 1980s for DNA [176]. Several vaccines including influenza and diphtheria toxoid have been delivered using powder injectors in animal models [177,178,179,180] and DNA vaccines for hepatitis B in humans [181,182]. Powder injection devices have been approved for delivery of lidocaine for local anesthesia.…”

Section: Discussionmentioning

confidence: 99%

Engineering Approaches to Transdermal Drug Delivery: A Tribute to Contributions of Prof. Robert Langer

Mitragotri

2013

Skin Pharmacol Physiol

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Transdermal drug delivery continues to provide an advantageous route of drug administration over injections. While the number of drugs delivered by passive transdermal patches has increased over the years, no macromolecule is currently delivered by the transdermal route. Substantial research efforts have been dedicated by a large number of researchers representing varied disciplines including biology, chemistry, pharmaceutics and engineering to understand, model and overcome the skin's barrier properties. This article focuses on engineering contributions to the field of transdermal drug delivery. The article pays tribute to Prof. Robert Langer, who pioneered the engineering approach towards transdermal drug delivery. Over a period spanning nearly 25 years since his first publication in the field of transdermal drug delivery, Bob Langer has deeply impacted the field by quantitative analysis and innovative engineering. At the same time, he has inspired several generations of engineers by collaborations and mentorship. His scientific insights, innovative technologies, translational efforts and dedicated mentorship have transformed the field.

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Epidermal powder immunization of mice and monkeys with an influenza vaccine

Cited by 49 publications

References 24 publications

Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand

Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand

Microneedle Vaccination with Stabilized Recombinant Influenza Virus Hemagglutinin Induces Improved Protective Immunity

Engineering Approaches to Transdermal Drug Delivery: A Tribute to Contributions of Prof. Robert Langer

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