Epidermal Stem Cells in Hair Follicle Cycling and Skin Regeneration: A View From the Perspective of Inflammation

Morgun, E. I.; Vorotelyak, E. A.

doi:10.3389/fcell.2020.581697

Cited by 32 publications

(32 citation statements)

References 176 publications

(261 reference statements)

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“…Acute trauma has been shown to stimulate resident cells to regenerate in previous studies. [158][159][160] Interestingly, a recent study reported that NP cells derived from trauma patients showed higher adipogenic and chondrogenic potential than those derived from degenerated IVDs. 161 Thus, we are more inclined to hypothesize that ECs, pericytes, and NPPCs are rare in the IVD, and acute trauma may induce local regeneration, which accounts for the unwanted distribution variability across donors.…”

Section: Discussionmentioning

confidence: 99%

Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs

Zhu

et al. 2021

Bone Res

144

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A comprehensive understanding of the cellular heterogeneity and molecular mechanisms underlying the development, homeostasis, and disease of human intervertebral disks (IVDs) remains challenging. Here, the transcriptomic landscape of 108 108 IVD cells was mapped using single-cell RNA sequencing of three main compartments from young and adult healthy IVDs, including the nucleus pulposus (NP), annulus fibrosus, and cartilage endplate (CEP). The chondrocyte subclusters were classified based on their potential regulatory, homeostatic, and effector functions in extracellular matrix (ECM) homeostasis. Notably, in the NP, a PROCR+ resident progenitor population showed enriched colony-forming unit-fibroblast (CFU-F) activity and trilineage differentiation capacity. Finally, intercellular crosstalk based on signaling network analysis uncovered that the PDGF and TGF-β cascades are important cues in the NP microenvironment. In conclusion, a single-cell transcriptomic atlas that resolves spatially regulated cellular heterogeneity together with the critical signaling that underlies homeostasis will help to establish new therapeutic strategies for IVD degeneration in the clinic.

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Section: Discussionmentioning

confidence: 99%

Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs

Zhu

et al. 2021

Bone Res

144

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“…The strong regenerative and healing capabilities of skin are intimately linked to the existence of skin stem cells. The epidermal stem cells (EPSC) are a type of autologous adult stem cells that exist in the epidermis and the hair follicles ensuring the preservation of adult skin hemostasis and hair regeneration; moreover, EPSC participates in the repair of the injured epidermis [ 3 ]. Typically, the remaining skin stem cells upon the wound surface are linked to a faster curing rate and particularly less scar formation.…”

Section: Introductionmentioning

confidence: 99%

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway

et al. 2021

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Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality, and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to the rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose-derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups [control (unwounded), sham (wounded but non-diabetic), diabetic, PRP-treated, ADSC-treated, and PRP+ADSCs-treated groups]. The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen, and angiogenesis. Moreover, gene and protein expression of key elements of the Notch signaling pathway (Notch1, Delta-like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes1), Hey1, Jagged-1), gene expression of angiogenic marker (vascular endothelial growth factor and stromal cell-derived factor 1) and epidermal stem cells (EPSCs) related gene (ß1 Integrin) were assessed. Results Our data showed better wound healing of PRP+ADSCs compared to their individual use after 7 and 14 days as the combined therapy caused reepithelialization and granulation tissue formation with a marked increase in area percentage of collagen, epidermal thickness, and angiogenesis. Moreover, Notch signaling was significantly downregulated, and EPSC proliferation and recruitment were enhanced compared to other treated groups and diabetic groups. Conclusions These data demonstrated that PRP and ADSCs combined therapy significantly accelerated healing of diabetic wounds induced experimentally in rats via modulating the Notch pathway, promoting angiogenesis and EPSC proliferation.

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“…The strong regenerative & healing capabilities of skin are intimately linked to the existence of skin stem cells. The skin stem cells (predominantly epidermal stem cells (EPSCs) and hair follicle stem cells) are considered as important sources of cells for skin healing, regeneration, and metabolism and are located in the basal layer of the epidermis and also, in the hair follicle bulge which contains the most potent EPSCs (3). Typically, the more the remaining skin stem cells upon the wound surface, the particularly faster the curing rate, and the particularly less the scar formation.…”

Section: Introductionmentioning

confidence: 99%

Adipose Mesenchymal Stem Cells Combined with Platelet Rich Plasma Accelerate Diabetic Wound Healing by Modulating Notch Pathway

Ebrahim

Dessouky

Mostafa

et al. 2021

Preprint

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Background Diabetic foot ulceration is a serious chronic complication of diabetes mellitus characterized by high disability, mortality and morbidity. Platelet-rich plasma (PRP) has been widely used for diabetic wound healing due to its high content of growth factors. However, its application is limited due to rapid degradation of growth factors. The present study aimed to evaluate the efficacy of combined adipose derived mesenchymal stem cells (ADSCs) and PRP therapy in promoting diabetic wound healing in relation to the Notch signaling pathway. Methods Albino rats were allocated into 6 groups (control, sham, diabetic, PRP-treated, ADSCs-treated and PRP+ADSCs-treated groups). The effect of individual and combined therapy was evaluated by assessing wound closure rate, epidermal thickness, dermal collagen and angiogenesis. Moreover, gene and protein expression of key elements of Notch signaling pathway (Notch1, Delta like canonical Notch ligand 4 (DLL4), Hairy Enhancer of Split-1 (Hes1), Hey1, Jagged-1), gene expression of angiogenic marker (Vascular endothelial growth factor & stromal cell-derived factor 1) and epidermal stem cells (EPSCs) related gene (ß1 Integrin) were assessed. Results Our data showed a strong wound-healing effect of PRP+ADSCs compared to their individual use after 7 and 14 days. Combined therapy caused marked increase in area percentage of collagen, epidermal thickness and angiogenesis. Moreover, Notch signaling was significantly down-regulated, EPSCs proliferation and recruitment was enhanced compared to other treated groups and diabetic group. Conclusions These data demonstrated that PRP and ADSCs combined therapy significantly accelerated healing of diabetic wounds induced experimentally in rats via modulating Notch pathway, promoting angiogenesis and EPSCs proliferation.

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Epidermal Stem Cells in Hair Follicle Cycling and Skin Regeneration: A View From the Perspective of Inflammation

Cited by 32 publications

References 176 publications

Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs

Spatially defined single-cell transcriptional profiling characterizes diverse chondrocyte subtypes and nucleus pulposus progenitors in human intervertebral discs

Adipose mesenchymal stem cells combined with platelet-rich plasma accelerate diabetic wound healing by modulating the Notch pathway

Adipose Mesenchymal Stem Cells Combined with Platelet Rich Plasma Accelerate Diabetic Wound Healing by Modulating Notch Pathway

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