Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

Xing, Kai; Chen, Yu; Wang, Liang; Lv, Xueze; Li, Zheng; Qi, Xiaolong; Wang, Xiangguo; Xiao, Longfei; Ni, Hemin; Guo, Yong; Sheng, Xihui

doi:10.1016/j.psj.2021.101558

Cited by 13 publications

(3 citation statements)

References 64 publications

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“…More recently, extracellular vesicles were found in chicken seminal plasma with different size and proteins markers between fertile and sub fertile males (Cordeiro et al, 2021). Another promising topic is the differences in miRNAs in high-and low-quality semen samples, miRNA patterns can be different depending on the male status and environmental changes, and differences between high and low motility samples were recently described in roosters (Xing et al, 2022). Further work comparing the composition of seminal plasma and the presence of different patterns of miRNAs of ejaculates between different storage time and between old and young males in the houbara bustard would be of great interest.…”

Section: Discussionmentioning

confidence: 99%

Pre-but not post-meiotic senescence affects sperm quality and reproductive success in the North African houbara bustard

et al. 2022

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Age-dependent reduction in reproductive success can arise due to multiple factors including a deterioration of reproductive physiology. Senescing males have been shown to produce ejaculates with poor sperm quality, which impinges on male reproductive success. In addition to individual age, gamete age can also affect male reproductive success. Accordingly, variance in male reproductive success can be due to pre-meiotic (referring to individual age) and post-meiotic senescence (sperm age). Here, we tested whether male senescence and sperm cell aging have additive or interactive effects on male reproductive success in a bird with a promiscuous mating system, the North African houbara bustard. To assess the effect of pre-meiotic aging, we compared male reproductive success between two age classes (3-6- and 12–16-year-old). To infer the effect of post-meiotic aging, male ejaculates were collected at three-time intervals following a common initial collection (day 1, 5, and 10). Therefore, day 1 ejaculates are supposed to contain younger sperm than day 5 and 10 ejaculates. Following controlled artificial inseminations, reproductive success was assessed using three fitness-linked traits (hatching success, chick growth rate and survival). In addition to reproductive output, we also assessed whether pre- and post-meiotic aging affected a wide range of sperm and ejaculate traits. In agreement with previous reports, we found that males in the older age class produced less sperm with poorer motility compared to young individuals. However, contrary to the prediction, we found that ejaculates collected at day 5 and 10 tended to have better sperm traits such as motility and velocity. The results on sperm traits were generally mirrored in the effect on reproductive success since young males produced offspring that grew faster and had better survival during the first month of life, and eggs fertilized by sperm collected at day 5 had the highest hatching success. In any of the models, there was evidence for interactive effects of male and sperm age. Overall, these results confirm the role of pre-meiotic aging on male reproductive success. The lack of evidence for sperm aging could come from the experimental design but might also reflect the pattern of mating frequency in a species with a lek-based mating system.

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Section: Discussionmentioning

confidence: 99%

Pre-but not post-meiotic senescence affects sperm quality and reproductive success in the North African houbara bustard

et al. 2022

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“…The finding that sperm motility in the drakes of HVS group was higher than in the LVS group of the present study also confirmed this point. The important role of sperm motility in the reproduction of poultry has been repeatedly recognized [ 34 , 35 , 36 ]. These results suggested that epididymis could affect the reproductive performance of drakes by regulating semen volume.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Provided a New Insight into the Molecular Mechanisms of Epididymis Regulating Semen Volume in Drakes

Ouyang

Tang

et al. 2022

Animals

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Semen volume is an important factor in artificial insemination (AI) of ducks. In drakes, seminal plasma that is produced by the epididymis determines the semen volume. However, the mechanism of epididymis regulating semen volume of drakes remains unclear. Therefore, the aim of the present study was to preliminarily reveal the mechanism regulating the semen volume through comparing the epididymal histomorphology and mRNA expression profiles between drakes with high-volume semen (HVS) and low-volume semen (LVS). Phenotypically, drakes in the HVS group produced more sperm than drakes in the LVS group. In addition, compared with the HVS group, the ductal square of ductuli conjugentes (DC) and dutus epididymidis (DE) in epididymis was significantly smaller in the LVS group, and the lumenal diameter and epithelial thickness of DC/DE were significantly shorter in the LVS group. In transcriptional regulation, 72 different expression genes (DEGs) were identified from the epididymis between HVS and LVS groups. Gene Ontology (GO) analysis indicated that the DEGs were mainly related to hormone secretion, neurotransmitter synthesis/transport, transmembrane signal transduction, transmembrane transporter activity, and nervous system development (p < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis showed that the DEGs were significantly enriched in pathways associated with hormone and neurotransmitter transmission (p < 0.05). In addition, further analysis of the top five pathways enriched by KEGG, nine key candidate genes (including SLC18A2, SNAP25, CACNA1B, GABRG2, DRD3, CAMK2A, NR5A1, and STAR) were identified, which could play a crucial role in the formation of semen. These data provide new insights into the molecular mechanism regulating semen volume of drakes and make feasible the breeding of drakes by semen volume.

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“…The transportation of sperm through vas deferens and sperm storage within the female reproductive tract is important for gaining and maintaining fertility ( Orr and Zuk, 2012 ; Xing et al, 2022 ). Extracellular vesicles transport miRNAs in both paracrine (between cells of the same origin) and endocrine (to distant targets cells) ways ( Yu et al, 2016b ).…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells

Han,

Li,

Zong

et al. 2023

Poultry Science

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Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters

Cited by 13 publications

References 64 publications

Pre-but not post-meiotic senescence affects sperm quality and reproductive success in the North African houbara bustard

Pre-but not post-meiotic senescence affects sperm quality and reproductive success in the North African houbara bustard

Comparative Transcriptome Analysis Provided a New Insight into the Molecular Mechanisms of Epididymis Regulating Semen Volume in Drakes

Extracellular vesicle-coupled miRNA profiles of chicken seminal plasma and their potential interaction with recipient cells

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