Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Lima, Silmara Leticia Gonçalves; Soares, Airton Renan Bastos; Stalker, Leanne; Santos, Regiane R.; Domingues, Sheyla Farhayldes Souza

doi:10.1017/s096719942100006x

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…The EG has also been successfully used in fibroblasts and somatic tissues from other mammals, such as porcine (Y. Li et al, 2006) and collared peccaries (Borges et al, 2018). Also, EG has been successfully used in the cryopreservation of epididymal spermatozoa from domestic cats (Buranaamnuay, 2020) and the vitrification of the epididymal tail (Lima et al, 2021). Our data demonstrate the possibility of using both intracellular cryoprotectants (DMSO and EG) in different concentrations in the presence or absence of SUC in the cryopreservation of felid cells.…”

Section: Discussionmentioning

confidence: 99%

Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas

et al. 2022

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The loss of wild biodiversity has prompted the development of cryobanks, such as those of somatic cells. This is the reality of Pumas, wild felids of ecological importance that suffer from anthropogenic actions, population decline, and subsequent loss of genetic diversity. Somatic cell banks are a strategy for conserving population diversity. We compared different concentrations and types of intracellular cryoprotectants (dimethyl sulfoxide, DMSO; ethylene glycol, EG) associated with 0.2 M of sucrose (SUC) in the cryopreservation of the somatic cells of captive Pumas. The cells were cryopreserved by slow freezing with different solutions containing Dulbecco's modified Eagle's medium with 10% fetal bovine serum and varying concentrations of DMSO and EG in the absence or presence of SUC. The cells were analyzed for morphological characteristics, viability, proliferative activity, metabolic activity, and apoptosis levels. Cells maintained similar fusiform morphology before and after cryopreservation. There was no difference in viability, regardless of the reduction in the concentration and type of intracellular cryoprotectants and sucrose. Similarly, proliferative activity, metabolic activity, and apoptosis levels were not altered by the composition of the cryoprotectants. In summary, we demonstrate that reducing the concentration of DMSO or EG ensures adequate cryopreservation of Puma somatic cells, regardless of the presence of SUC.

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Section: Discussionmentioning

confidence: 99%

Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas

et al. 2022

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“…The purpose of the study was to determine the effect of the vitrification of epididymal cauda by comparing the effects of glycerol and ethylene glycol on epididymal sperm quality post-vitrification. The authors observed that epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with ethylene glycol as the cryoprotectant [ 30 ].…”

Section: New Approachesmentioning

confidence: 99%

Canine and Feline Testicular Preservation

Silva

2022

Animals

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The increased interest in breeding dogs and cats and their use as models for other canids and felids demand research to improve reproductive techniques. Among them, testicular cryopreservation stands out. Testicular cryopreservation enables the maintenance of reproductive capacity and allows the establishment of germplasm banks for several species of commercial value or at risk of extinction. Furthermore, it enables the transport of genetic material among different regions. It is noteworthy that this biotechnology represents the only possibility of preserving the fertility of prepubertal animals that have died, so it has great importance in the propagation of the genetic material of animals. The spermatogonia present in the testes can be cultivated in vitro and the sperm obtained can be used in artificial reproduction programs. Although advances have been achieved with the use of testicular fragments to obtain viable and functional germ cells, the establishment of protocols that can be used in clinical routine have not been concluded yet. The testicular cryopreservation process can be carried out through techniques such as slow freezing, fast freezing and vitrification. However, the protocols used for the canine and feline species are still in the experimental phase. Given the importance of the topic, the aim of this review is to draw a profile of the subject approaching the main works on testicular cryopreservation in dogs and cats.

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Epididymal spermatozoa from domestic cats in assisted reproduction biotechniques: Perspectives for wild felid applications

Lima,

Leão,

Reis

et al. 2024

Theriogenology Wild

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Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Cited by 5 publications

References 38 publications

Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas

Comparison between concentration and type of intracellular cryoprotectants and the presence of sucrose for cryobanks of somatic cells derived from captive Pumas

Canine and Feline Testicular Preservation

Epididymal spermatozoa from domestic cats in assisted reproduction biotechniques: Perspectives for wild felid applications

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