Epididymal α- l -fucosidase and its possible role in remodelling the surface of bull spermatozoa

Aguilera, Andrea Carolina; Boschin, Verónica; Robina, Inmaculada; Elías‐Rodríguez, Pilar; Sosa, Miguel A. Gama

doi:10.1016/j.theriogenology.2017.08.016

Cited by 2 publications

(3 citation statements)

References 37 publications

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“…Reproductive aging may accelerate after depletion of Bxy‐fuca , resulting in reduced total number of eggs laid. In Aberdeen angus , the α‐L‐fucosidases, which present in the epididymal fluid, removing the fucose from the spermatozoa, and this defucolation is necessary for sperm maturation . The α‐L‐fucosidases have been implicated for gamete binding during fertilization in mice and fruit flies .…”

Section: Discussionmentioning

confidence: 99%

“…In Aberdeen angus, the -L-fucosidases, which present in the epididymal fluid, removing the fucose from the spermatozoa, and this defucolation is necessary for sperm maturation. 62 The -L-fucosidases have been implicated for gamete binding during fertilization in mice 63 and fruit flies. 53 The tight binding ability of mouse sperm to the zona pellucida and oolemma is significantly decreased after blocking the enzyme with anti-fucosidase antibody.…”

Section: Biological Roles Of Bxy-fuca In B Xylophilusmentioning

confidence: 99%

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Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Tang

Zhu

et al. 2019

Pest Management Science

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BACKGROUND The pine wood nematode (PWN) Bursaphelenchus xylophilus is the causal agent of pine wilt disease (PWD). This disease is a serious threat to pine forests globally. The fuca gene encodes α‐L‐fucosidase, which plays crucial roles in numerous biological and pathological processes in bacteria, fungi, plants and animals. To find promising control strategies against PWD, we investigated the expression and functions of Bxy‐fuca in B. xylophilus. RESULTS Bxy‐fuca encoding α‐L‐fucosidase is highly conserved within the deduced functional domains and key residues. It is expressed continuously across all developmental stages of B. xylophilus. mRNA in situ hybridization demonstrated that Bxy‐fuca was mainly localized in the body wall muscles and intestine. RNA interference indicated that the zygotic expression of Bxy‐fuca was indispensable for embryogenesis. The rate of B. xylophilus egg hatch was significantly decreased after Bxy‐fuca was interfered. Postembryonic silence of Bxy‐fuca resulted in a dramatic decrease in the longevity of and the total number of eggs produced by B. xylophilus. In addition, the motility of the nematode was greatly hampered with a significant drop in head thrashing frequency. CONCLUSION Bxy‐fuca plays crucial roles in development, lifespan and reproduction of B. xylophilus. Our results provide promising hints for control of PWD by blocking embryogenesis and ontogenesis, decreasing nematode fecundity, and disrupting the connection between B. xylophilus and its vector beetle by preventing nematode movement into the tracheal system. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Biological Roles Of Bxy-fuca In B Xylophilusmentioning

confidence: 99%

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Tang

Zhu

et al. 2019

Pest Management Science

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“…During epididymal transit and ejaculation, several proteins bind to the spermatozoa (Sullivan, 2016; Sullivan et al., 2015), which modify sperm membrane and become the cells fertile and mobile (Aguilera et al., 2017; Westfalewicz et al., 2017b). Proteins of the seminal plasma have several physiological functions (i.e.…”

Section: Introductionmentioning

confidence: 99%

Seminal plasma insulin‐like growth factor I and total protein concentration in peripubertal period of the Gyr bulls

Souza¹,

Martins

Emerick

et al. 2021

Reprod Domestic Animals

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This study aimed to evaluate the correlation of seminal plasma IGF‐I and total proteins from peripubertal bulls with semen characteristics and onset of puberty. Sixteen dairy Gyr bulls were separated into early (n = 8) and regular (n = 8) groups, according to the onset of puberty. Semen was monthly collected by electroejaculation from 14 to 26 months of age, and the onset of puberty was retrospectively determined (17.0 ± 1.6 and 19.2 ± 1.5 months, to early and regular group, respectively). Five time points were evaluated (day −60, day −30, day 0, day 30 and day 60 days of puberty), being day 0 considered as beginning of puberty. Scrotal circumference and body weight were also assessed. Semen characteristics were evaluated, the seminal plasma was separated by centrifugation and total protein and IGF‐I concentrations were determined. There was no difference between groups to concentration of the seminal plasma total proteins, but we found an interaction between group and age. Seminal plasma IGF‐I concentrations were not different between early and regular groups; thus, the data were combined for analysis. Combined IGF‐I concentrations were positively correlated with sperm motility and concentration, age, body weight and scrotal circumference. Negative correlation was found between IGF‐I concentration and total sperm defects. Increased IGF‐I was observed in day −30 and day 0 of puberty in early and regular groups, respectively. Seminal plasma total proteins are involved in peripubertal modifications and IGF‐I from Zebus dairy bulls can influence the seminal characteristics and the growth factor increase occurs concomitantly with body growth but cannot be used to define puberty bulls earlier.

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Epididymal α- l -fucosidase and its possible role in remodelling the surface of bull spermatozoa

Cited by 2 publications

References 37 publications

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Bxy‐fuca encoding α‐L‐fucosidase plays crucial roles in development and reproduction of the pathogenic pinewood nematode, Bursaphelenchus xylophilus

Seminal plasma insulin‐like growth factor I and total protein concentration in peripubertal period of the Gyr bulls

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