Epididymis Ligation: A Minimally Invasive Technique for Preparation of Teaser Rams

Tamadon, Amin; Nikahval, Behrooz; Sepehrimanesh, Masood; Mansourian, Mahboubeh; Naeini, Aboutorab Tabatabaei; Nazifi, Saeed

doi:10.1111/j.1532-950x.2009.00617.x

Cited by 14 publications

(10 citation statements)

References 18 publications

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“…The mucosal surface was washed with saline buffer and the mucosal injury was macroscopically assessed using Morris et al (19) grading scale. Colon tissue was processed and stained according to the previously reported procedures (10,(20)(21)(22)(23)(24)(25). Briefly, colon tissues were free of surrounding fat and fixed in 10% buffered formalin solution for histopathological examination.…”

Section: Tissue Sampling Macroscopic and Microscopic Evaluationsmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Effects of Gel and Aqueous Extract of Melilotus officinalis L. in Induced Ulcerative Colitis: A Rattus norvegicus Model

et al. 2015

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Background: Ulcerative colitis (UC) is a chronic condition of intestine illness accompanied by some unknown etiology with different immune, genetic and environmental factors. Objectives: The current study aimed to evaluate the antioxidant and anti-inflammatory effects of the Melilotus officinalis L. in the acetic acid induced UC in rats. Materials and Methods:The plant aqueous extraction and high performance liquid chromatography and Ferric Reducing Antioxidant Power (FRAP) assay were performed on aqueous extract to identify its compounds and antioxidant activities. Also, 70 adult male rats were and UC was induced using 3% acetic acid solution. They received different daily doses of M. officinalis L. in two forms (orally, 500 and 1000 mg/kg) and gel extract (10% and 20%). On the 7th day, the colon tissues were examined regarding the macroscopic and histopathology lesions plus oxidative stress and compared to the positive and negative control groups. Results: HPLC analysis revealed that five grams of the flower powder contained 9.7 mg gallic acid, 99 mg catechin, 21.9 mg caffeic acid, 0.86 mg chlorogenic acid, 1.13 mg quercetin, 548.9 mg cinnamic acid, 289 mg coumarin and 126 mg p-coumaric acid. The FRAP value of the extract was 2.91 ± 0.14 μM/g. There were significant differences between the group of rats which received the gel or aqueous extract of the flower compared to the negative control group using normal saline and the base gel and they had no significant differences with the positive control group using the Asacol, regarding the pathologic, malondialdehyde, and weight improvements. Conclusions: It can be concluded that the M. officinalis L. extract can be used as an effective medicine to treat UC in animal model and also in human subjects.

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Section: Tissue Sampling Macroscopic and Microscopic Evaluationsmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Effects of Gel and Aqueous Extract of Melilotus officinalis L. in Induced Ulcerative Colitis: A Rattus norvegicus Model

et al. 2015

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“…For all the animals, the distal 10 cm portion of the colon was removed for histological evaluation. Tissue processing and section preparation were performed according to previous studies (21)(22)(23). The degree of inflammation of the colon was graded as described before (24).…”

Section: Histopathological Evaluationmentioning

confidence: 99%

The Healing Effect of Hypericum perforatum Extract on Acetic Acid-Induced Ulcerative Colitis in Rat

Tanideh¹,

Nematollahi²,

Hosseini³

et al. 2014

Ann Colorectal Res

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Background: Ulcerative colitis (UC) is a form of inflammatory bowel disease (IBD). There are several chemical and herbal drug regimens for treatment of UC. Objectives: The aims of this study were to investigate the effects of Hypericum perforatum on histopathological and tissue malondialdehyde (MDA) level of colonic tissue in rat with induced UC. Materials and Methods: Two milliliters of 3% acetic acid was administered into the colon to induce UC. Seventy rats were divided into seven equal groups. Groups I and II received 1 mL of 600 and 300 mg/kg H. perforatum extract orally per day respectively; groups III and IV received 1 mL of 20% and 10% intra-colonic gel form of H. perforatum extract daily respectively; group V as positive control received 2 mL of intra-colonic asacol; group VI was a negative control receiving 0.5 mL/kg of normal saline after induction of UC; group VII received just intra-colonic gel base. All the animals were evaluated for histological changes and tissue MDA level seven days after the treatment. Results: H. perforatum extract in the two forms of trans-rectal and oral administration on the seventh day after the therapy could result in a more healing effect on acetic acid-induced damaged colonic tissue with a reduction in the MDA activity. In trans-rectal administration, the 20% gel form had a better healing response than the 10% gel form and was prominently more effect on the seventh day of the therapy. In oral administration of strawberry extract, the 600 mg/kg dosage had a better healing response than the 300 mg/kg and was significantly more effective on the seventh day of therapy. Conclusions: So H. perforatum may be considered as a treatment of choice for UC especially in gel form to broaden the current therapy options of the disease.

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“…Se encontró que todos los animales manifestaron libido; esta observación fue semejante a la reportada por múltiples estudios (7,8,11,(13)(14)(15). La manifestación de la libido de los machos del estudio fue alta (100 %) si se compara con lo enunciado por Grandeth, Noriega y Suárez (17), quienes describen que el 40 % de los animales puestos en servicio después de ser sometidos a la técnica de desmotomía peneana posterior en toros receladores presentaron respuesta ferohormonal negativa.…”

Section: Discussionunclassified

“…Con base en esto se considera que los resultados tienen grandes ventajas con relación a los referidos por Rondón et al (18), quienes encontraron que cuatro de los animales intervenidos con la técnica de implantación lateral del prepucio en toros cebuínos mostraron capacidad de copular, dado a que los animales desarrollaron determinadas habilidades en los movimientos del salto que les permitieron reaprender a realizar la cópula. Así mismo, la técnica del estudio tiene ventajas sobre la ligadura del epidídimo realizadas por Tramadon et al (13), puesto que los ani-males quedan posibilitados para realizar la cópula, con lo cual corren el riesgo de adquirir enfermedades de trasmisión sexual.…”

Section: Discussionunclassified

Técnica de Mastoby para preparar ovinos receladores

2017

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ResumenEsta investigación tuvo como objetivo desarrollar una técnica quirúrgica para preparar ovinos receladores. Se planteó porque las técnicas practicadas en la sabana cordobesa colombiana no han mostrado resultados satisfactorios, ya que en el posoperatorio los ovinos presentan dificultad para cicatrizar, larga convalecencia y considerable costo económico. Por este motivo, muchos propietarios optan por el sacrificio del animal, lo que además incrementa las pérdidas del productor. Para este trabajo se utilizó un grupo de 10 ovinos criollos con edad y peso promedio de 1 año y 21 kg, clínicamente sanos. Para la intervención quirúrgica, los ovinos fueron tranquilizados con maleato de acepromacina a dosis de 0,02 mg/kg por vía intravenosa y la anestesia se consiguió con la aplicación epidural de roxicaína al 2 % sin epinefrina en la región intercoccígea (Co2-Co3) a dosis de 1 mL/5 kg de peso vivo. Luego de conseguir el bloqueo de la zona, se practicó la téc-nica quirúrgica que tuvo una duración promedio de 3 min. Los animales intervenidos fueron evaluados por 6 meses; se observó buena cicatrización y capacidad para realizar la micción. En cuanto a la libido, todos tuvieron un comportamiento positivo frente a las hembras en celo. El 100 % de los animales fueron incapaces de penetrar a las hembras; solo el 10 % de ellos tuvo salida parcial del pene (glande). La técnica propuesta fue efectiva, poco invasiva, rápida, fácil de practicar y económica. No requiere instrumental especializado y no presenta complicaciones posoperatorias, lo cual permite introducir a recelaje a los ovinos a los 15 días posintervención.Palabras clave: estro, ovinos, receladores, técnica quirúrgica. Mastoby technique for the preparation of teaser rams AbstractThis research aimed to develop a surgical technique to prepare teaser rams. This was proposed because the techniques practiced in the Colombian savanna of the Córdoba region have not shown satisfactory results, since in the postoperative period sheep present difficulty to heal and long convalescence, which has a considerable economic cost. For this reason, many owners opt for sacrificing the animal, which also increases the losses of producers. For this work, a group of ten clinically healthy Creole sheep was used, with an average age of 1 year and an average weight of 21 kg. For the surgery, sheep were tranquilized with intravenous acepromazine maleate at a dose of 0.02 mg/kg, and anesthesia was achieved with the epidural injection of 2% roxicaine without epinephrine in the dorsal intercoccygeal region (Co2-Co3) at a dose of 1 mL/5 kg of live weight. After successfully blocking the area, the surgical technique was performed, which lasted an average of 3 min. The operated animals were evaluated during 6 months; good healing and ability to urinate was observed. As for libido, all had a positive behavior in front of females in heat. 100% of the animals were unable to penetrate the females; only 10% of them had partial erection of the penis (glans). The proposed technique was effective, non-i...

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Epididymis Ligation: A Minimally Invasive Technique for Preparation of Teaser Rams

Abstract: This method is simply performed, without skin wounds, and minimal postoperative care is needed. The technique should be readily adaptable to other species.

Cited by 14 publications

References 18 publications

Antioxidant and Anti-Inflammatory Effects of Gel and Aqueous Extract of Melilotus officinalis L. in Induced Ulcerative Colitis: A Rattus norvegicus Model

Antioxidant and Anti-Inflammatory Effects of Gel and Aqueous Extract of Melilotus officinalis L. in Induced Ulcerative Colitis: A Rattus norvegicus Model

The Healing Effect of Hypericum perforatum Extract on Acetic Acid-Induced Ulcerative Colitis in Rat

Técnica de Mastoby para preparar ovinos receladores

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