Epigallocatechin‐3‐gallate exhibits anti‐inflammatory effects in a human interface dermatitis model—implications for therapy

Abstract

Background Epigallocatechin-3-gallate (EGCG) has been proven effective in treating viral warts. Since anticarcinogenic as well as anti-inflammatory properties are ascribed to the substance, its use has been evaluated in the context of different dermatoses. The effect of EGCG on interface dermatitis (ID), however, has not yet been explored. Objectives In this study, we investigated the effect of EGCG on an epidermal human in vitro model of ID.Methods Via immunohistochemistry, lesional skin of lichen planus patients and healthy skin were analysed concerning the intensity of interferon-associated mediators, CXCL10 and MxA. Epidermal equivalents were stained analogously upon ID-like stimulation and EGCG treatment. Monolayer keratinocytes were treated likewise and supernatants were analysed via ELISA while cells were processed for vitality assay or transcriptomic analysis.Results CXCL10 and MxA are strongly expressed in lichen planus lesions and induced in keratinocytes upon ID-like stimulation. EGCG reduces CXCL10 and MxA staining intensity in epidermis equivalents and CXCL10 secretion by keratinocytes upon stimulation. It furthermore minimizes the cytotoxic effect of the stimulus and downregulates a magnitude of typical pro-inflammatory cytokines that are crucial for the perpetuation of ID. ConclusionsWe provide evidence concerning anti-inflammatory effects of EGCG within a human in vitro model of ID.The capacity to suppress mediators that are centrally involved in disease perpetuation suggests EGCG as a potential topical therapeutic in lichen planus and other autoimmune skin diseases associated with ID.

Superoxide Dismutase-Mimetic Polyphenol-Based Carbon Dots for Multimodal Bioimaging and Treatment of Atopic Dermatitis

“…Polyphenols naturally derived phytochemical compounds have been reported in the potential treatment of AD. , These compounds have multiple hydroxylated aromatic rings that exert anti-inflammatory effects and scavenge ROS similar to superoxide dismutase (SOD). Among the variety of polyphenols, epigallocatechin-3-gallate (EGCG) has been recently effective in AD treatment. , In the AD mouse model, EGCG treatment significantly alleviates the disruption of the skin barrier by inhibiting the macrophage migration inhibitory factor (MIF) and various cytokines (e.g., IL-2, IL-12 p40, TNF-α, IFN-γ, etc.) related to AD pathogenesis .…”

“…Among the variety of polyphenols, epigallocatechin-3gallate (EGCG) has been recently effective in AD treatment. 9,10 In the AD mouse model, EGCG treatment significantly alleviates the disruption of the skin barrier by inhibiting the macrophage migration inhibitory factor (MIF) and various cytokines (e.g., IL-2, IL-12 p40, TNF-α, IFN-γ, etc.) related to AD pathogenesis.…”

EGCG identified as an autophagy inducer for rosacea therapy

“…Epigallocatechin-3-gallate (EGCG), a natural polyphenol found in green tea, has many biological activities, including anti-inflammatory, antioxidant, cardioprotective, neuroprotective, and anticancer activities (Nan et al, 2019;Yi et al, 2020;Nan et al, 2021). Studies have revealed EGCG as a potential therapeutic agent for various skin inflammation conditions, including psoriasiform dermatitis (Chamcheu et al, 2018), interface dermatitis (ID) (Braegelmann et al, 2022), and atopic dermatitis (AD) (Noh et al, 2008). Although a clinical trial of four healthy volunteers demonstrated the potential anti-angiogenic effect of EGCG cream (Domingo et al, 2010), whether EGCG has a therapeutic effect on rosacea remains unknown.…”

The Therapeutic Effects of Epigallocatechin-3-gallate and Rutin, either Alone or in Combination with Low-dose radiation, against Testicular Injury Evoked by Nicotine in Rats

“…For instance, epigallocatechin-3-gallate (EGCG) is the major catechin found in green tea. It is among natural products that have recently attracted the attention of researchers due to its antioxidant (Dewaantari et al, 2021), anti-inflammatory (Braegelmann et al, 2021), anti-apoptotic (Zong et al, 2021), and anticancer (Zhu et al, 2021) properties. Moreover, in several models, ECCG has shown to be renoprotective (Kanlaya & Thongboonkerd 2019), neuroprotective , and anti-diabetic (Li et al, 2020).…”