Epigallocatechin-3-Gallate Reduces Fat Accumulation in Caenorhabditis elegans

Abstract

Epigallocatechin gallate (EGCG) is a polyphenol that is abundant in green tea. It has been reported that consumption of EGCG can contribute to weight loss, however, the underlying mechanism is not fully understood. To determine how EGCG reduces body fat, an organism model Caenorhabditis elegans was used, which is a useful animal model system in exploring crucial biological mechanisms that are readily applicable to humans. In this study, different strains were raised for two days on Escherichia coli OP 50 diet with or without 100 μM and 200 μM EGCG treatment. The current results showed that 100 μM and 200 μM EGCG significantly reduced the triglyceride content of wild type worms by 10% and 20% (P-value<0.01 and <0.001, respectively) compared to the control, respectively, without affecting its food intake and physiological behaviors. Additionally, EGCG could effectively reduce fat accumulation in C. elegans dependent on atgl-1 (encoding a homolog of adipose triglyceride lipase), which suggests that EGCG controls the body fat by inhibiting adipogenesis.

Epigallocatechin‐3‐gallate activates the AMP‐activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes

“…However, the present study was limited, oil red O staining was not performed to further confirm the effect of EGCG on the lipid accumulation. Consistent with the study of organism model, Caenorhabditis elegans, 100 and 200 μM EGCG significantly reduced the TG content by 10% and 20% respectively compared to the control (Liu, Peng, Yue, Shen, & Park, 2018). However, the effect of EGCG on lipid metabolism in canine hepatocytes was completely abrogated by the BML-275.…”

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

“…The lipases, atgl-1 (a homolog of the human adipose triglyceride lipase or patatin-like phospholipase domain-containing protein 2, PNPLA2) and hosl-1 (a homolog of the human hormone-sensitive lipase E, LIPE), are targets for anti-obesity agents due to their effects on lipolysis ( Farias-Pereira et al., 2018 , Liu et al., 2018 , Machado et al., 2018 ). Treatment of 200 μM isorhamnetin upregulated atgl-1 expression by 27% ( P = 0.0211), while hosl-1 expression was upregulated only by 6% in the 100 μM isorhamnetin-treated worms ( P = 0.0106) compared to the control ( Fig.…”

“…The anti-obesity mechanisms of action of dietary bioactives and plant extracts on lipid metabolism have various molecular targets in C. elegans ( Farias-Pereira et al., 2018 , Lin et al., 2019 , Liu et al., 2018 , Machado et al., 2018 , Shen et al., 2017 , Shen et al., 2018c , Sun et al., 2016 , Yue et al., 2019 ). It was reported that the inhibitory effects of NHR-49 on fatty acid desaturases (lipogenesis) were related to the fat-lowering effects of a saponin extract in C. elegans ( Lin et al., 2019 ).…”

“…Human lipid metabolism pathways are conserved in C. elegans , which help to identify the mechanisms of action of food components prior to vertebrate animals and/or humans ( Shen et al., 2018a , Shen et al., 2018b ). Previously, studies have examined the anti-obesity effects of plant extracts and food bioactive components in C. elegans , showing the relevance of this animal model for the mechanistic studies of prospective bioactives ( Bhattacharya et al., 2013 , Farias-Pereira et al., 2018 , Farias-Pereira et al., 2020 , Lin et al., 2019 , Liu et al., 2018 , Machado et al., 2018 , Shen et al., 2017 , Shen et al., 2018a , Shen et al., 2018b , Shen et al., 2018c , Sun et al., 2016 , Yue et al., 2019 ). Thus, the goal of this study was to determine the effects of isorhamnetin on lipid metabolism by using C. elegans as an animal model.…”

Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans

“…Dietary polyphenols, such as quercetin and epigallocatechin-3-gallate, are claimed to play a role in attenuating or preventing the onset of aging-related diseases. 24,25 Excess fat accumulation and abnormal metabolism cause or exacerbate numerous diseases, such as type 2 diabetes and certain types of cancer. 26 As a facile and accurate method, Oil Red O staining was adopted to assess major fat stores in C. elegans.…”