“…Besides the effect of androgen deprivation therapy (ADT) on the progression of PCA by surgical castration of TRAMP mice [41,42], the effects of many agents with chemopreventive potential including green tea [26,43], NSAIDs [44,45], flutamide [46], retinoic acid [47], vitamin E analog [48], genistein [24,49], epigallocatechin-3-gallate (EGCG) [50], silibinin [23,51], dietary restriction [52] and immunotherapy [53,54], have been studied using this model. While most of the publications reported anti-cancer effects of their test compounds, El Touny et al showed that feeding TRAMP mice (TRAMP-FVB) with a diet containing 0.25% genistein from 12 to 20 WOA induced an aggressive progression of PCA, as evidenced by a 16% increase in the number of WD and PD prostates, coinciding with a 70% incidence of pelvic lymph node metastases as opposed to 0% in the control group [49].…”