Epigallocatechin Gallate (EGCG), a Green Tea Polyphenol, Reduces Coronavirus Replication in a Mouse Model

Park, Rackhyun; Jang, Minsu; Park, Yea-In; Jung, WooChul; Park, Jayhyun; Park, Junsoo

doi:10.3390/v13122533

Cited by 39 publications

(29 citation statements)

References 25 publications

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“…The activity of EGCG against SARS-CoV-2 has been attributed, at least in part, to disrupting binding to its receptor, angiotensin-converting enzyme 2 (ACE2) 10 , 11 . Interestingly, EGCG was also shown to inhibit HCoV-OC43 infection 8 , 11 , 12 . The in vivo efficacy of EGCG was highlighted in a murine model of HCoV-OC43 infection in which EGCG-fed mice had reduced viral burden in the lungs compared to untreated mice 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Interestingly, EGCG was also shown to inhibit HCoV-OC43 infection 8 , 11 , 12 . The in vivo efficacy of EGCG was highlighted in a murine model of HCoV-OC43 infection in which EGCG-fed mice had reduced viral burden in the lungs compared to untreated mice 12 . However, HCoV-OC43 does not bind the ACE2 receptor (a proposed target of EGCG against SARS-CoV-2 10 , 11 ), suggesting the involvement of alternative or additional antiviral mechanisms against CoVs, which are still unknown.…”

Section: Introductionmentioning

confidence: 99%

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The green tea catechin EGCG provides proof-of-concept for a pan-coronavirus attachment inhibitor

LeBlanc

Colpitts

2022

Sci Rep

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The COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has emphasized the serious threat to human health posed by emerging coronaviruses. Effective broadly-acting antiviral countermeasures are urgently needed to prepare for future emerging CoVs, as vaccine development is not compatible with a rapid response to a newly emerging virus. The green tea catechin, epigallocatechin gallate (EGCG), has broad-spectrum antiviral activity, although its mechanisms against coronavirus (CoV) infection have remained unclear. Here, we show that EGCG prevents human and murine CoV infection and blocks the entry of lentiviral particles pseudotyped with spike proteins from bat or highly pathogenic CoVs, including SARS-CoV-2 variants of concern, in lung epithelial cells. Mechanistically, EGCG treatment reduces CoV attachment to target cell surfaces by interfering with attachment to cell-surface glycans. Heparan sulfate proteoglycans are a required attachment factor for SARS-CoV-2 and are shown here to be important in endemic HCoV-OC43 infection. We show that EGCG can compete with heparin, a heparan sulfate analog, for virion binding. Our results highlight heparan sulfate as a conserved cell attachment factor for CoVs, and demonstrate the potential for the development of pan-coronavirus attachment inhibitors, which may be useful to protect against future emerging CoVs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The green tea catechin EGCG provides proof-of-concept for a pan-coronavirus attachment inhibitor

LeBlanc

Colpitts

2022

Sci Rep

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“…There is mounting evidence suggesting the therapeutic potential of green tea catechins in prevention/treatment of COVID19. In particular, in addition to numerous docking studies (for instance, [1][2][3][4][5][6][7]) there is growing number of studies showing direct antiviral activity of at least one catechin epigallocatechin-3-gallate (EGCG) against SARS-CoV-2 in in vitro experiments ( [8][9][10][11][12][13][14][15][16][17] see also [18][19][20] for review). Moreover, a recent work [21] reports that EGCG from green tea effectively blocks infection of SARS-CoV-2 and new variants.…”

Section: Introductionmentioning

confidence: 99%

COVID-19 and per capitagreen tea consumption: update

Storozhuk

2022

Preprint

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Purpose: In spite of the development of numerous vaccines for the prevention of COVID-19 and approvement of several drugs for its treatment, there is still a great need in effective and inexpensive therapy of this disease. Pharmacological evidence suggesting the therapeutic potential of green tea catechins in amelioration/treatment of COVID19 is growing rapidly, however, there are only a few epidemiological studies addressing this possibility. The aim of this study was to provide update regarding ecological study assessing this issue as of January 2021. Methods: The methodological approach used in this report is similar to that described previously. Briefly, information about COVID-19 morbidity (defined as a total number of cases per million population) and mortality (defined as a total number of deaths per million population) for a specific date was directly obtained from Worldometers info. Coronavirus. Analysis was restricted to 134 countries or territories with at least 3 million population. Twenty-one of these countries/territories, with estimated per/capita green tea consumption above 150 g (annually), were considered as a group with the high consumption. Countries/territories with the estimated per/capita green tea consumption below 150 g (N=82) were considered as the group with low the consumption. Results: Pronounced differences in COVID-19 morbidity and mortality between groups of countries with high and low green tea consumption were found as of February 20, 2022. These differences were still observed in a subset of countries with HDI above 0.55. Moreover, in this restricted subset of countries, weak but statistically significant correlations between COVID-19 morbidity (or mortality) and per/capita green tea consumption were observed in a multiple regression model accounting for: population density, percentage of population aged above 65, and percentage of urban population. Conclusion: The obtained results provide additional, though indirect, support of the idea that green tea catechins can be useful for treatment/amelioration of COVID-19. These results are in line with emerging evidence from other studies, including pharmacological. Nevertheless, further research is necessary to directly validate or reject this idea.

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“…They were proven to be highly stable, similarly compacted, and subject to low conformational variability [ 110 ]. EGCG, found in different plant sources including Camellia sinensis , Vitis vinifera , and black garlic extract, was further investigated in several in vitro and in vivo studies to test its effectiveness against COVID-19 [ 131 , 132 , 133 , 134 ]. EGCG and its oxidized form were found to inhibit Mpro in vitro [ 133 , 134 ], to directly inhibit an early infection [ 132 ], and to reduce viral replication in mouse lung cells [ 132 ].…”

Section: Phytochemicals As a Reservoir To Search For Sars-cov-2 Mpro ...mentioning

confidence: 99%

The Main Protease of SARS-CoV-2 as a Target for Phytochemicals against Coronavirus

Issa

Сокорнова

Zhidkin

et al. 2022

Plants

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In late December 2019, the first cases of COVID-19 emerged as an outbreak in Wuhan, China that later spread vastly around the world, evolving into a pandemic and one of the worst global health crises in modern history. The causative agent was identified as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Although several vaccines were authorized for emergency use, constantly emerging new viral mutants and limited treatment options for COVID-19 drastically highlighted the need for developing an efficient treatment for this disease. One of the most important viral components to target for this purpose is the main protease of the coronavirus (Mpro). This enzyme is an excellent target for a potential drug, as it is essential for viral replication and has no closely related homologues in humans, making its inhibitors unlikely to be toxic. Our review describes a variety of approaches that could be applied in search of potential inhibitors among plant-derived compounds, including virtual in silico screening (a data-driven approach), which could be structure-based or fragment-guided, the classical approach of high-throughput screening, and antiviral activity cell-based assays. We will focus on several classes of compounds reported to be potential inhibitors of Mpro, including phenols and polyphenols, alkaloids, and terpenoids.

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Epigallocatechin Gallate (EGCG), a Green Tea Polyphenol, Reduces Coronavirus Replication in a Mouse Model

Cited by 39 publications

References 25 publications

The green tea catechin EGCG provides proof-of-concept for a pan-coronavirus attachment inhibitor

The green tea catechin EGCG provides proof-of-concept for a pan-coronavirus attachment inhibitor

COVID-19 and per capitagreen tea consumption: update

The Main Protease of SARS-CoV-2 as a Target for Phytochemicals against Coronavirus

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