Epigallocatechin gallate induces chemopreventive effects on rats with diethylnitrosamine‑induced liver cancer via inhibition of cell division cycle 25A

Abstract

epigallocatechin gallate (eGcG), the most active monomer in green tea (GT), has demonstrated potential therapeutic and preventive effects on various tumors, including liver cancer. However, the anticancer mechanisms of eGcG in liver cancer remain to be elucidated. The abnormal expression of cell division cycle 25A (CDC25A) has been identified in liver cancer and is closely associated with malignancy and poor prognosis in patients with hepatocellular carcinoma (Hcc). The present study used human hepatoma cell lines and rats with diethylnitrosamine (den)-induced Hcc as models to investigate the association between the effect of eGcG on liver cancer and regulation of the p21waf1/cip1/cdc25a axis. The results demonstrated that eGcG can inhibit the proliferation of HepG2 and Huh7 cells, reduce the expression of cdc25a and increase the expression of p21waf1/cip1 in HepG2. In vivo, Hcc was induced by den in Sprague-dawley rats. EGCG significantly reduced tumor volume and improved the survival rates of rats with Hcc. The expression levels of cdc25a mrna and protein in liver tissues and the level of serum γ glutamyl transpeptidase in rats treated with eGcG were significantly decreased, while p21waf1/cip1 mrna and protein expression levels were increased compared with the Hcc group, in the process of den-induced Hcc. no significant difference in the chemopreventive effects on liver cancer was observed between GT extract and eGcG under an eGcG equivalence condition. Thus, eGcG can suppress human hepatoma cell proliferation and prolong the survival of rats with Hcc, and the potential mechanism may be involved in eGcG-induced upregulation of p21waf1/cip1 and downregulation of cdc25a.

Onco-Preventive and Chemo-Protective Effects of Apple Bioactive Compounds

“…Notwithstanding the limitation with their bioavailability, catechins and epicatechins have been reported to have a multitude of anticancer properties summarized in Table 3. Both catechins and epicatechins in vitro and in vivo have inhibited cancer cell proliferation [226][227][228], induced cell cycle arrest by upregulating the expression of p21 [229] and inhibition of CDC25A [226], induced apoptosis [227,[229][230][231][232][233], and reduced invasion and migration [233].…”

Biochemical, hematological, peripheral smear and weight evaluation of low dose Epigallocatechin Gallate in Diethylnitrosamine administered rats

“…In many studies, in accordance with our study, it was determined that DEN application caused an increase in liver enzyme activity. [24][25][26][27][28][29] The erythropoietin (EPO) hormone increases in conditions where oxygen intake is reduced, such as hypoxia, chronic obstructive pulmonary disease (COPD) and climbing to high altitudes. [30] In our study, the amount of EPO was tried to be measured, but could not be measured because it was outside the limits that can be measured by the device.…”

The Emerging Role of Stress Granules in Hepatocellular Carcinoma

“…Alternatively, the delivery of siRNAs (i.e., aptamers) to reduce the expression of these SGs nucleating proteins, i.e., G3BP1, may represent an additional approach as demonstrated in colorectal cancer (CRC) [159]. Resveratrol [160], or epigallocatechin-gallate (EGCG) [161] were, for example, reported to reduce G3BP1 expression in lung (H1299 and CL13) cancer cells [162,163], while attenuating NAFLD [164] and restraining HCC development [165]. However, given the pleiotropic effects of these compounds in the cells, how G3BP1 inhibition contributes to their anti-tumoral activity remains to be evaluated.…”