Epigallocatechin gallate inhibits sphere formation of neuroblastoma BE(2)-C cells

Nishimura, Noriyuki; Hartomo, Tri Budi; Pham, Thi Van Huyen; Lee, Myeong Jin; Yamamoto, Tomoto; Morikawa, Satoru; Hasegawa, Daiichiro; Takeda, Hiroyuki; Kawasaki, Keiichiro; Kosaka, Yoshiyuki; Yamamoto, Nobuyuki; Kubokawa, Ikuko; Mori, Takeshi; Yanai, Tomoko; Hayakawa, Akira; Takeshima, Yasuhiro; Iijima, Kazumoto; Matsuo, Masafumi; Nishio, Hisahide

doi:10.1007/s12199-011-0239-5

Cited by 33 publications

(26 citation statements)

References 28 publications

(33 reference statements)

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“…Our study showed that the expression levels of OCT4 strongly correlated with the stemness of NB I-type cells BE (2)-C. High expression of OCT4 increases grades and correlates with tumor progression and malignancy in glioma, a nervous system malignant tumor (8). With the overexpression of OCT4, BE (2)-C cells acquired increased proliferation rate and formed relatively more and larger sphere colonies on soft agar, indicating that OCT4 also played a role in increasing malignancy in BE (2)-C. This is consistent with a previous study that indicates BE (2)-C spheres grown in serum-free non-adherent culture show increased expression of OCT4 (16). In addition, we showed that with the downregulation of OCT4, BE (2)-C grew slowly compared with control, and was unable to form colonies on soft agar.…”

Section: Discussionsupporting

confidence: 90%

“…OCT4 has been used as a cell marker in NB to identify progenitor cells (12)(13)(14)(15)(16)(17). Our study showed that the expression levels of OCT4 strongly correlated with the stemness of NB I-type cells BE (2)-C. High expression of OCT4 increases grades and correlates with tumor progression and malignancy in glioma, a nervous system malignant tumor (8).…”

Section: Discussionmentioning

confidence: 62%

“…Recent findings demonstrated that OCT4 also plays an oncogenic role and is expressed in some cancer stem cells (8)(9)(10)(11). Furthermore, previous studies detected the expression of OCT4 in NB primary samples, metastatic bone marrow aspirates, cell lines, and OCT4 is facilitated to identify NB cancer stem cells as the stemness gene (12)(13)(14)(15)(16)(17). OCT4 is proved to have a potential role in maintaining NB cancer stem cell niche localized in the hypoxic zones of the solid tumors in vivo (12) and is used for identification of tumor-derived endothelial cells as a putative marker (17).…”

Section: Introductionmentioning

confidence: 99%

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Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells

Yang

Zheng

et al. 2013

Oncology Reports

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Abstract. In in vitro continuous culture, N (neuroblastic)-, S (substrate adherent)-and I (intermediate)-type cells were identified in human neuroblastoma (NB), where I-type is recognized as a stem cell type. Octamer-binding protein 4 (OCT4) is a cell marker used to identify the stemness of cells, whose roles in regulating I-type NB cells have yet to be fully elucidated. In the present study, we assessed the differentiation regulation role of OCT4 in BE (2)-C of I-type cells. We demonstrated that downregulation of OCT4 expression in BE (2)-C was associated with reduced cell proliferation and loss of colony formation ability on soft agar, and prompted BE (2)-C cells to differentiate to S-type cells. By contrast, overexpression of OCT4 increased cell proliferation and colony formation ratio, but no obvious differentiation promotion was observed. Furthermore, induced differentiation of BE (2)-C cells to S-type by 5-bromo-2'-deoxyuridine (BrdUrd) was accompanied by reduced expression of OCT4.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 99%

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Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells

Yang

Zheng

et al. 2013

Oncology Reports

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“…Green tea is a popular drink that is consumed by millions of people worldwide on a daily basis. In previous studies, EGCG demonstrated high cytotoxicity in cancer cells compared with normal cells and inhibited sphere formation by neuroblastoma cells . Takahashi A et al .…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin gallate hinders human hepatoma and colon cancer sphere formation

Wubetu

Shimada

Morine

et al. 2015

J of Gastro and Hepatol

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“…Neuroblastoma spheres were cultured as previously described (23). Briefly, neuroblastoma cells were cultured at 37˚C (5% CO 2 and 95% air) in sphere medium consisting of DMEM/Ham's F12 (3:1, Invitrogen), 100 units/ml penicillin/streptomycin (PC/SM; Invitrogen), 2% B27 supplement (Invitrogen), 40 ng/ml basic fibroblast growth factor (bFGF; R&D Systems, Minneapolis, MN), and 20 ng/ml epidermal growth factor (EGF; R&D Systems), subcultured by non-enzymatic cell dissociation solution (Sigma, St. Louis, MO), and maintained for >4 weeks in Ultra-low attachment culture dishes (CORNING, Corning, NY).…”

Section: Methodsmentioning

confidence: 99%

Rab15 alternative splicing is altered in spheres of neuroblastoma cells

et al. 2012

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Abstract. Neuroblastoma is an aggressive pediatric tumor that accounts for 15% of cancer-related deaths in children. More than half of high-risk neuroblastoma patients develop tumor relapse that is lethal in most cases. A small population of tumor-initiating cells (TICs), recently identified from high-risk neuroblastoma patients as spheres, is believed to be responsible for tumor relapse. Rab family small G proteins are essential in controlling membrane traffic and their misregulation results in several cancers. Rab15 was originally isolated as a brain-specific Rab protein regulating the endocytic recycling pathway and was recently identified as a downstream target of the neural transcription factor Atoh1. Previously, we identified two alternatively spliced Rab15 isoforms in neuroblastoma cells and showed a significant correlation between Rab15 expression and neuronal differentiation. As aberrant alternative splicing is intimately associated with an increasing number of cancers, its use as a new diagnostic and/or prognostic biomarker has attracted considerable attention. In the present study, we explored cancer-associated changes of Rab15 alternative splicing in neuroblastoma TICs. We found that Rab15 alternative splicing generated two novel isoforms designated as Rab15 AN2 and Rab15 AN3 in addition to two known isoforms designated as Rab15 CN and Rab15 AN1. Although both Rab15 AN2 and Rab15 AN3 contained premature termination codons, they were detected in not only neuroblastoma cells but also in normal human tissues.One isoform was predominantly expressed in the brain and testis, while the other isoform was more specifically expressed in the brain. In neuroblastoma, Rab15 isoform balance measured by the Rab15 CN /Rab15 AN1+AN2+AN3 ratio was significantly decreased in spheres compared to parental cells. These results suggest that Rab15 alternative splicing may serve as a biomarker to discriminate TICs from non-TICs in neuroblastoma.

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Epigallocatechin gallate inhibits sphere formation of neuroblastoma BE(2)-C cells

Abstract: The present results suggest that EGCG may inhibit the development of TICs in BE(2)-C cells.

Cited by 33 publications

References 28 publications

Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells

Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells

Epigallocatechin gallate hinders human hepatoma and colon cancer sphere formation

Rab15 alternative splicing is altered in spheres of neuroblastoma cells

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