Epigallocatechin Gallate Restrains Angiogenesis and Glycolysis in Colorectal Cancer via the Circular Ribonucleic Acid Actin Gamma 2/Micro Ribonucleic Acid-370-5p Pathway

Abstract

To figure out the pathway by which epigallocatechin gallate restrains angiogenesis and glycolysis in colorectal cancer through the circular ribonucleic acid (actin gamma 2)/microRNA-370-5p axis. Detection of circular actin gamma 2 and microRNA-370-5p in colorectal cancer patient tissues and cells and vascular endothelial growth factor A in colorectal cancer cells was conducted. Cell viability and proliferation, angiogenesis, glucose and lactate of colorectal cancer cells were examined. Circular actin gamma 2 and microRNA-370-5p's combination link was verified. Epigallocatechin gallate restrained cell viability, angiogenesis and glycolysis in colorectal cancer cells. In tissues and cells of colorectal cancer patients, circular actin gamma 2 was elevated but microRNA-370-5p was reduced. Elevation of circular actin gamma 2 or repression of microRNA-370-5p motivated vascular endothelial growth factor A, cell proliferation, angiogenesis and glycolysis in colorectal cancer cells. Meanwhile, it was demonstrated that circular actin gamma 2 competitively combined with microRNA-370-5p. MicroRNA-370-5p inhibitor turned around the repressive effect of epigallocatechin gallate and knockdown of circular actin gamma 2 on colorectal cancer progression. Circular actin gamma 2 competitively combines with microRNA-370-5p to control colorectal cancer progression and epigallocatechin gallate restrains colorectal cancer angiogenesis and glycolysis through the circular actin gamma 2/microRNA-370-5p pathway.

Lactate-related metabolic reprogramming and immune regulation in colorectal cancer

“…Interestingly, the clinical use of LDH inhibitors is limited by the interaction of other drugs with low selectivity or complex cellular components, and the heterogeneity of LDH expression between different cancers requires more cell type-specific studies in this field. Epigallocatechin gallate inhibited CRC angiogenesis and lactate production in glycolysis through Circular Ribonucleic Acid Actin Gamma 2/Micro Ribonucleic Acid-370-5p Pathway and reduced CRC cell viability ( Table 1 ) ( 144 ).…”