Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Bi, Wen‐Lu; Shukla, Mukund R.; Ren, Li; Hamborg, Zhibo; Blystad, Dag‐Ragnar; Saxena, Praveen K.; Wang, Qiao‐Chun

doi:10.3390/plants10091889

Cited by 43 publications

(29 citation statements)

References 126 publications

(259 reference statements)

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“…These results were similar to the 48% and 53% of regrowth levels obtained from the optimized Dv and V cryo-foil protocol, respectively, indicating the cryo-recovered plants of present study derived from shoot tips with surviving AD and LPs. The direct regrowth observed from the present study would ensure the genetic stability of cryo-recovered plants, as indirect regrowth through callus may result in somaclonal variation (Miguel and Marum, 2011;Wang et al, 2021a).…”

Section: Discussionmentioning

confidence: 71%

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

Wang

Jiang

et al. 2021

Preprint

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Improvements of existing cryopreservation protocols are necessary to facilitate long-term preservation of plant germplasm and the cryotherapy-effect of pathogen eradication. This study reported a vitrification (V) cryo-foil/plate methods for cryopreservation of shoot tips and cryotherapy effect in ‘Pink Lady’ apple. In V cryo-foil/plate protocols, shoot tips were first attached onto aluminum foils/plates using calcium alginate before other procedures. Shoot tips cryopreserved by V cryo-foil required 6.1 weeks to fully recover and 53% of shoot regrowth was obtained, comparable to the Dv cryopreservation. Similar regrowth levels were produced between applying V cryo-foil and Dv cryopreservation to another 4 Malus genotypes. Histological observations in shoot tips cryopreserved by Dv and V cryo-foil found only those with surviving apical dome and leaf primordia (LPs) could recover after cryopreservation. In apical meristem of shoot tips cryopreserved by Dv and V cryo-foil, higher surviving probability was detected from the V cryo-foil protocol, and the young LPs showed the highest level of surviving. Virus detection in cryo-derived plants showed apple stem grooving virus and apple chlorotic leaf spot virus were all preserved after cryopreservation, and higher eradication efficiency of apple stem pitting virus (70%) was produced by Dv than the 55% of V cryo-foil. These results supported applying V cryo-foil as an improvement to the widely applied Dv method in shoot tip cryopreservation, and also revealed a seesaw mode between shoot recovery and cryotherapy effect. Once the seesaw moves to increase the recovery after cryopreservation, the cryotherapy-effect on the other side would be decreased.

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Section: Discussionmentioning

confidence: 71%

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

Wang

Jiang

et al. 2021

Preprint

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“…1A). It is well documented that in vitro tissue culture imposes stressful conditions and induces the generations of ROS, thus resulting in ROS-induced oxidative stress (Kaczmarczyk et al 2012;Funnekotter et al 2017;Bednarek and Orłowska 2020;Wang et al 2021b; Fig. 1B, F).…”

Section: Ros Generationmentioning

confidence: 91%

“…In vitro tissue culture has become an integral part of the cryopreservation technology currently used for the establishment and maintenance of stock cultures, and the postculture process for the recovery of cryopreserved tissues (Funnekotter et al 2017;Wang et al 2021b; Fig. 1A).…”

Section: Ros Generationmentioning

confidence: 99%

ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation

Ren

Wang

2021

Planta

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Main conclusion Reactive oxygen species (ROS)-induced oxidative stress results in low success or even total failure of cryopreservation. Better understanding of how the plant establishes resistance/tolerance to ROS-induced oxidative stress facilitates developments of robust cryopreservation procedures. Abstract Cryopreservation provides a safe and efficient strategy for long-term preservation of plant genetic resources. ROS-induced oxidative stress caused damage to cells and reduced the ability of the plant to survive following cryopreservation, eventually resulting in low success or even total failure. This paper provides updated and comprehensive information obtained in the past decade, including the following: (1) ROS generations and adaptive responses of antioxidant systems during cryopreservation; (2) expressions of oxidative stress-associated genes and proteins during cryopreservation; (3) ROS-triggered programmed cell death (PCD) during cryopreservation; and (4) exogenous applications of enzymatic and non-enzymatic antioxidants in improving success of cryopreservation. Prospects for further studies are proposed. The goal of the present study was to facilitate better understanding of the mechanisms by which the plant establishes resistance/tolerance to oxidative stress during cryopreservation and promote further studies toward the developments of robust cryopreservation procedures and wider application of plant cryobiotechnology.

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“…Cryopreservation results in the exposure of cells to chemical, physical, and physiological stresses. Cryoinjury caused by freezing/thawing (in slow-cooling) and cooling/rewarming (in vitrification-based modern techniques) may influence the DNA, cell membrane structure and function, molecular and subcellular functions, totipotency, and finally regrowth of entire plants and their field performance [ 174 ]. Some of the most important problems when exposing cells to cryopreservation include osmotic injury during dehydration, hyperhydration during recovery, toxicity of cryoprotective agents/mixtures, oxidative damage induced by reactive oxygen species (ROS), and secondary lipid peroxidation aldehyde products [ 35 , 175 , 176 ].…”

Section: Cryopreservation and Omics Technologiesmentioning

confidence: 99%

Cryopreservation of Endangered Ornamental Plants and Fruit Crops from Tropical and Subtropical Regions

Kaviani

Kulus

2022

Biology

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Horticultural crops comprise various economic species extending from fruits, nuts, vegetables, spices and condiments, ornamentals, aromatic, and medicinal plants. Ornamental and fruit plants are produced mainly for their nutritional and aesthetic values, respectively. Unfortunately, many tropical and subtropical species are in danger of extinction because of climate change and (a)biotic stresses. It is imperative to preserve the germplasms of these species for the present and future genetic improvement programs. Cryopreservation, i.e., maintenance of tissues at the ultralow temperature of liquid nitrogen, is a promising long-term preservation technique, alternative to seed or in vitro banks, which can be applied for both vegetatively and generatively (through seeds) propagated crops, including those with recalcitrant seeds. It is a technology of choice not only for the preservation of plant biodiversity but also for virus elimination in the proficient administration of large-scale micropropagation. The main advantages of cryopreservation are the lowering of in vitro culture expenditures, needed space, contamination risk, and operator errors. However, tropical species are temperature delicate and one of the foremost challenging issues is preconditioning treatments that stimulate physiological reactions to sufficiently enhance tolerance to dehydration and cryogenic procedures. In recent years, several cryopreservation methods based on encapsulation-vitrification, droplet-vitrification, the use of aluminum cryo-plates, and cryo-mesh have been established. Combined cryo-techniques, gene/DNA conservation, as well as studies on perceiving bio-molecular events and exploring the multistage process from the beginning to end of cryopreservation are receiving more emphasis. The development of cryobiomics delivers a conceptual framework to assess the significance of cell signaling mechanisms on cellular functions, the influence of cryoinjury factors on sample viability, and the implications for genetic stability following cryo-storage. The aim of this mini-review article is to provide a succinct synthesis of the developed cryogenic procedures and their use for the storage and exchange of genetic resources of tropical and subtropical horticultural crops, particularly fruit crops and ornamental plants under the threat of extinction.

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Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Cited by 43 publications

References 126 publications

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

ROS-induced oxidative stress in plant cryopreservation: occurrence and alleviation

Cryopreservation of Endangered Ornamental Plants and Fruit Crops from Tropical and Subtropical Regions

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