Epigenetic and somaclonal divergence in Dendrocalamus farinosus for physiological augmentation and lignin degradation

Abstract

Epigenetic and molecular variation is a key approach for the improvement of plants. From in vitro tissue culture of Dendrocalamus farinosus; 30 plants were regenerated and after 2 years, the physiological, biochemical, and genomic studies were conducted. The results highlighted that for all phenotypic characteristics, the 30 regenerated plants were superior in comparison with the control (CK). From genetical analysis, a total of 97 bands were witnessed ranging between 212 bp and 2.2 kb. The results for OPU14 showed one additional specific band (723 bp) and one band (700 bp) were missing. The 10 plants were having genetic variability and can be termed as somaclones while the other plants have epigenetic variations. The cellulose and lignin analysis highlighted that somaclone No. 30 has the least cellulose content of 35%; while the somaclones No. 102 and No. 213 have the least 3.21% and 3.48% of lignin contents. Therefore, somaclones No. 30, No. 102, and No. 213 were selected for the histochemical localization. The lignin investigation revealed that somaclone No. 30 is greater while somaclones No. 102 and No. 213 were reduced in vascular bundles in comparison with the CK along with the high expression level of 4CL, C3H, C4H, COMT, and CCoAOMT1 genes.

Genome-wide analysis and characterization of Dendrocalamus farinosus SUT gene family reveal DfSUT4 involvement in sucrose transportation in plants

“…The SUT proteins play key roles in the translocation of sucrose from the Deng et al 10.3389/fpls.2022.1118398 source to the sink through the phloem to support plant growth and development (Slewinski and Braun, 2010;Deol et al, 2013). D. farinosus is an ideal economic crop in southwestern China because of its high fiber content and dual usage for food and paper pulp (Chen et al, 2021;Imran et al, 2022;Pei et al, 2022). Sucrose is the main substrate of fiber synthesis, and studying the function of SUT genes in regulating sucrose transportation helps to illustrate the synthesis mechanism of fiber in plants (Yadav et al, 2022)-for example, GhSUT1 and GhSUT3 are crucial to regulate sucrose accumulation in cotton fibers (Zhang et al, 2017;Yadav et al, 2022).…”

“…D. farinosus is one of the essential economic bamboo species in southwestern China, which has many advantages including high growth rate and disease resistance, cold and drought tolerance, and dual usage for food and timber (Zakikhani et al, 2014;Chen et al, 2021;Pei et al, 2022). In particular, D. farinosus is also an excellent paper pulp bamboo species because of its high fiber content, stiffness, and low density (Xiong et al, 2012;Imran et al, 2022). Sucrose is the predominant form of transported carbon, which is necessary to synthesize fiber (Rennie and Turgeon, 2009), and SUT transporter genes are crucial to carbon delivery and fiber formation in some plants (Zhang et al, 2017;Yadav et al, 2022).…”

Genome-Wide Identification and Expression Analysis of Dendrocalamus farinosus CCoAOMT Gene Family and the Role of DfCCoAOMT14 Involved in Lignin Synthesis

“…Moreover, bamboo resembles wood in appearance, weight, hardness, texture and strength, making bamboo an ideal substitute for many traditional wood products. D. farinosus is an important sympodial bamboo species for both bamboo shoots and materials; it is mainly distributed in southwest China, with the advantages of high fiber content, a large biomass, cold tolerance, rapid growth and high yield, which are also important for paper making and ecological greening [4].…”