Epigenetic Clues to Better Understanding of the Asexual Embryogenesis in planta and in vitro

Pasternak, Taras; Dudits, Dénes

doi:10.3389/fpls.2019.00778

Cited by 21 publications

(17 citation statements)

References 33 publications

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“…present a highly specialized asexual reproductive mechanism, based on the symmetric plantlet development along the leaf margins or leaf tips of adult plants (Figure 1) [12,16]. Such clonal-spreading reproductive mechanism is driven by a complex phenomenon that combines both embryogenic and organogenetic events that has not been fully elucidated to date [17][18][19][20][21]. Both the metabolic and reproductive patterns found on Bryophyllum sp.…”

Section: Introductionmentioning

confidence: 99%

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

et al. 2020

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The subgenus Bryophyllum includes about 25 plant species native to Madagascar, and is widely used in traditional medicine worldwide. Different formulations from Bryophyllum have been employed for the treatment of several ailments, including infections, gynecological disorders, and chronic diseases, such as diabetes, neurological and neoplastic diseases. Two major families of secondary metabolites have been reported as responsible for these bioactivities: phenolic compounds and bufadienolides. These compounds are found in limited amounts in plants because they are biosynthesized in response to different biotic and abiotic stresses. Therefore, novel approaches should be undertaken with the aim of achieving the phytochemical valorization of Bryophyllum sp., allowing a sustainable production that prevents from a massive exploitation of wild plant resources. This review focuses on the study of phytoconstituents reported on Bryophyllum sp.; the application of plant tissue culture methodology as a reliable tool for the valorization of bioactive compounds; and the application of machine learning technology to model and optimize the full phytochemical potential of Bryophyllum sp. As a result, Bryophyllum species can be considered as a promising source of plant bioactive compounds, with enormous antioxidant and anticancer potential, which could be used for their large-scale biotechnological exploitation in cosmetic, food, and pharmaceutical industries.

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Section: Introductionmentioning

confidence: 99%

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

et al. 2020

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“…Mesophyll protoplast re-programming includes two steps [ 2 ]. The first step involves a significant reprogramming of the nucleus, including chromatin relaxation [ 38 ], and is accompanied by the upregulation of enzymes related to nuclear architecture (e.g., RPL3a, RPL13a). Nuclear activation is related to cell preparation for cell division, as confirmed by DNA replication events.…”

Section: Resultsmentioning

confidence: 99%

Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation

Pasternak

Paponov

Kondratenko

2021

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Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant growth, plating efficiency, and promotion of shoot regeneration. Protocols have been established for the detection of all three steps in plant regeneration: (i) chromatin relaxation and activation of auxin biosynthesis, (ii) cell cycle progression, and (iii) conversion of cell-cycle active cells to totipotent ones. The competence for cell division was detected by DNA replication events and required high cell density and high concentrations of the auxinic compound 2,4-D. Cell cycle activity and globular structure formation, with subsequent shoot induction, were detected microscopically and by labeling with fluorescent dye Rhodamine123. The qPCR results demonstrated significantly upregulated expression of the genes responsible for nuclear reorganization, auxin responses, and auxin biosynthesis during the early stage of cell reprogramming. We further optimized cell reprogramming with this protocol by applying glutathione (GSH), which increases the sensitivity of isolated mesophyll protoplasts to cell cycle activation by auxin. The developed protocol allows us to investigate the molecular mechanism of the de-differentiation of somatic plant cells.

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“…For instance, zygotic embryogenesis is an essential process for plant reproduction and allows the regeneration of a complete plant from a single cell through the fertilization process [2]. In a similar manner, somatic embryogenesis (SE) is an alternative regeneration process, for which a new individual is generated from a vegetative cell [3,4], occurring naturally in some Kalanchoe species [5]. It can also be manipulated by in vitro tissue culture under appropriate stress signals such as plant growth regulators and wounding, as well as other mechanisms [5,6], offering multiple possibilities to investigate the bases and potential applications of SE in economically important crops.…”

Section: Introductionmentioning

confidence: 99%

“…In a similar manner, somatic embryogenesis (SE) is an alternative regeneration process, for which a new individual is generated from a vegetative cell [3,4], occurring naturally in some Kalanchoe species [5]. It can also be manipulated by in vitro tissue culture under appropriate stress signals such as plant growth regulators and wounding, as well as other mechanisms [5,6], offering multiple possibilities to investigate the bases and potential applications of SE in economically important crops. In general, SE is well-known at the morphological, physiological, and molecular level in plant model species such as Arabidopsis thaliana [7][8][9], Medicago truncatula [10], and others, such as Coffea canephora [11].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of Somatic Embryogenesis-Related Transcription Factors in Cultivated Strawberry (Fragaria × ananassa) and Evolutionary Relationships among Rosaceae Species

2021

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Somatic embryogenesis is a plant regeneration method commonly used in tissue culture. Its molecular mechanisms are well-known in model plants such as Arabidopsis thaliana L. LEAFY COTYLEDON1 (LEC1), LEAFY COTYLEDON2 (LEC2), FUSCA3 (FUS3), ABSCISIC ACID INSENSITIVE3 (ABI3), and BABYBOOM (BBM) genes are considered master regulators in the induction, growth, and maturation of somatic embryos. However, the study of these transcription factors in fruit crops with high agronomic and economic value such as cultivated strawberry (Fragaria × ananassa Duch.) and other Rosaceae species is scarce. The purpose of this study was the in silico characterization of LEC1, ABI3, FUS3, LEC2, and BBM(LAFL-B) genes from F. × ananassa genome and the study of the evolutionary relationships within the Rosaceae family. Synteny analyses and molecular evolutionary rates were performed to analyze the evolution of each transcription factor within the Rosaceae family. Synteny was conserved between F. × ananassa and other Rosaceae genomes, and paralogous genes were selected through negative selection. Additionally, the exon–intron organization and multiple alignments showed that gene structure and DNA-binding domains were conserved in F. ×ananassa transcription factors. Finally, phylogenetic trees showed close evolutionary relationships between F. × ananassa and its orthologous proteins in the Rosoideae subfamily. Overall, this research revealed novel insights in the LAFL-B network in F. × ananassa and other species of the Rosaceae family. These results provide useful in silico information and new resources for the establishment of more efficient propagation systems or the study of ploidy effects on somatic embryogenesis.

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Epigenetic Clues to Better Understanding of the Asexual Embryogenesis in planta and in vitro

Cited by 21 publications

References 33 publications

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

From Ethnomedicine to Plant Biotechnology and Machine Learning: The Valorization of the Medicinal Plant Bryophyllum sp.

Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation

Genome-Wide Analysis of Somatic Embryogenesis-Related Transcription Factors in Cultivated Strawberry (Fragaria × ananassa) and Evolutionary Relationships among Rosaceae Species

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