Epigenetic dynamics during germline development: insights from Drosophila and C. elegans

Gleason, Ryan J; Chen, Xin

doi:10.1016/j.gde.2022.102017

Cited by 11 publications

(4 citation statements)

References 89 publications

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“…This might be due to the tight epigenetic regulation, which has been reported and is linked to the silencing of transgenes in the germline 29 . Although germline expression at low levels can be achieved from single-copy transgenes 30 , transgene arrays 31 , including hsp16 promoter-bearing ones 32 , have been previously shown to be vulnerable to silencing in the germline.…”

Section: Reprogramming At Reproductive Stages Leads To Bagging and Fo...mentioning

confidence: 92%

In vivo reprogramming ofCaenorhabditis elegansleads to heterogeneous effects on lifespan

Kamaludeen,

Mauge,

Mouridi

et al. 2024

Preprint

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In the last decade, cellular reprogramming of fully differentiated cells to pluripotent stem cells has become of great interest. Importantly, cellular reprogramming by expression of Oct4, Sox2, Klf4, and cMyc (OSKM) can ameliorate age-associated phenotypes in multiple tissues and extend lifespan in progeroid and aged wild-type mice. Surprisingly, the effects of in vivo reprogramming have not been deeply investigated in any other model organisms. Here, for the first time, we induce in vivo reprogramming in C. elegans using a heat-inducible system at multiple developmental and adult stages. Similar to mice, expression of the reprogramming factors leads to premature death with different levels of toxicity at distinct developmental stages and aging. In vivo reprogramming in C. elegans might represent a valuable tool to improve our understanding of development and in vivo reprogramming.

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Section: Reprogramming At Reproductive Stages Leads To Bagging and Fo...mentioning

confidence: 92%

In vivo reprogramming ofCaenorhabditis elegansleads to heterogeneous effects on lifespan

Kamaludeen,

Mauge,

Mouridi

et al. 2024

Preprint

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“…Furthermore, epigenetic mechanisms, such as chromatin remodeling and histone modifications, have been shown to play important roles in the male germline lineage (Tarayrah and Chen, 2013). In particular, multiple examples have shown the interplay between extrinsic signaling pathways and intrinsic epigenetic mechanisms in the Drosophila adult testes (Gleason and Chen, 2023; Vidaurre and Chen, 2021). For example, previous studies have shown that the H3K27me3 histone demethylase, U biquitously transcribed T etratricopeptiderepeat gene on the X chromosome (UTX), acts as a negative regulator of JAK-STAT signaling by maintaining the transcription of socs36E , whose proper expression and function maintain the balance between GSCs and CySCs (Amoyel et al, 2016; Issigonis et al, 2009; Singh et al, 2010; Tarayrah et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

TheDrosophilahistone methyl-transferase SET1 coordinates multiple signaling pathways in regulating male germline stem cell maintenance and differentiation

Vidaurre,

Song,

et al. 2024

Preprint

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Many cell types come from tissue-specific adult stem cells that maintain the balance between proliferation and differentiation. Here, we study how the H3K4me3 methyltransferase, Set1, regulates early-stage male germ cell proliferation and differentiation in Drosophila. Early-stage germline-specific knockdown of set1 results in a temporally progressed defects, arising as germ cell loss and developing to overpopulated early-stage germ cells. These germline defects also impact the niche architecture and cyst stem cell lineage in a non-cell-autonomous manner. Additionally, wild-type Set1, but not the catalytically inactive Set1, could rescue the set1 knockdown phenotypes, highlighting the functional importance of the methyl-transferase activity of the Set1 enzyme. Further, RNA-seq experiments reveal key signaling pathway components, such as the JAK-STAT pathway gene stat92E and the BMP pathway gene mad, that are upregulated upon set1 knockdown. Genetic interaction assays support the functional relationships between set1 and JAK-STAT or BMP pathways, as mutations of both the stat92E and mad genes suppress the set1 knockdown phenotypes. These findings enhance our understanding of the balance between proliferation and differentiation in an adult stem cell lineage. The germ cell loss followed by over-proliferation phenotypes when inhibiting a histone methyl-transferase raise concerns about using their inhibitors in cancer therapy.

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“…Some mechanisms (such as epigenetic regulation) exist to regulate the function of targets by affecting target expressions [ 41 ]. microRNAs and histone methylation regulation were identified to control transgenerational PS-NP toxicity [ 42 , 43 , 44 ].…”

Section: Introductionmentioning

confidence: 99%

Transgenerational Response of Germline Nuclear Hormone Receptor Genes to Nanoplastics at Predicted Environmental Doses in Caenorhabditis elegans

Liu,

Wang,

Bian

et al. 2024

Toxics

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Transgenerational nanoplastic toxicity could be detected in Caenorhabditis elegans after exposure at the parental generation (P0-G); however, the underlying mechanisms remain largely unclear. We aimed to examine the role of germline nuclear hormone receptors (NHRs) in controlling the transgenerational toxicity of polystyrene nanoparticles (PS-NPs) based on gene expression screening and functional analysis. Among germline NHR genes, daf-12, nhr-14, and nhr-47 expressions were increased and nhr-12 expression was decreased by PS-NPs (1 and 10 μg/L). Transgenerational alterations in expressions of these four NHR genes were also induced by PS-NPs (1 and 10 μg/L). RNAi of daf-12, nhr-14, and nhr-47 caused resistance, whereas RNAi of nhr-12 conferred susceptibility to transgenerational PS-NP toxicity. After PS-NP exposure, expressions of ins-3, daf-28, and ins-39 encoding insulin ligands, efn-3 encoding Ephrin ligand, and lin-44 encoding Wnt ligand, as well as expressions of their receptor genes (daf-2, vab-1, and/or mig-1), were dysregulated by the RNAi of daf-12, nhr-14, nhr-47, and nhr-12. Therefore, alteration in certain germline NHRs could mediate the induction of transgenerational nanoplastic toxicity by affecting secreted ligands and their receptors in the offspring of exposed organisms.

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Epigenetic dynamics during germline development: insights from Drosophila and C. elegans

Cited by 11 publications

References 89 publications

In vivo reprogramming ofCaenorhabditis elegansleads to heterogeneous effects on lifespan

In vivo reprogramming ofCaenorhabditis elegansleads to heterogeneous effects on lifespan

TheDrosophilahistone methyl-transferase SET1 coordinates multiple signaling pathways in regulating male germline stem cell maintenance and differentiation

Transgenerational Response of Germline Nuclear Hormone Receptor Genes to Nanoplastics at Predicted Environmental Doses in Caenorhabditis elegans

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