Epigenetic Mechanisms Regulate Innate Immunity against Uropathogenic and Commensal-Like Escherichia coli in the Surrogate Insect Model Galleria mellonella

Heitmueller, Miriam; Billion, André; Dobrindt, Ulrich; Vilcinskas, Andreas; Mukherjee, Krishnendu

doi:10.1128/iai.00336-17

Cited by 43 publications

(33 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, the larva of G. mellonella has been reported as an easy-to-use model organism for several pathogenic bacteria (11,12,38,39). This model host represents a valuable tool for the study of host-pathogen interactions because it is convenient for distinguishing virulent and avirulent strains and identifying bacterial virulence factors ΔtonB2 (which were preincubated with 10 M duck Hb), and duck Hb alone.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept

Liu

Huang

et al. 2019

Infect Immun

View full text Add to dashboard Cite

Galleria mellonella larvae have been used as a host model to study interactions between pathogens and hosts for several years. However, whether the model is useful to interrogate Riemerella anatipestifer infection biology remained unknown. This study aimed to exploit the potential of G. mellonella larvae and reveal their limitations as a host model for R. anatipestifer infection. G. mellonella larvae were shown to be effective for virulence evaluations of different R. anatipestifer strains. Furthermore, the virulent strain R. anatipestifer CH-1 had a stronger ability to proliferate than the attenuated strain R. anatipestifer ATCC 11845 in both G. mellonella larvae and ducklings. Unconventionally it was shown that G. mellonella larvae cannot be used to evaluate the efficacy of antimicrobials and their combinations. Additionally, it was shown that certain virulence factors, such as OmpA (B739_0861), B739_1208, B739_1343, and Wza (B739_1124), were specific only for ducklings, suggesting that G. mellonella larvae must be cautiously used to identify virulence factors of R. anatipestifer. Evaluation of heme uptake-related virulence genes, such as tonB1 and tonB2, required preincubating the strains with hemoglobin before infection of G. mellonella larvae since R. anatipestifer cannot obtain a heme source from G. mellonella larvae. In conclusion, this study revealed the applicability and limitations of G. mellonella as a model with which to study the pathogen-host interaction, particularly in the context of R. anatipestifer infection.A fter Robert Koch's work, Stanley Falkow established the molecular version of Koch's postulates, which have guided the study of the microbial virulence determinants in infectious diseases since the late 1980s (1). One of the key purposes of the molecular version of Koch's postulates is to test whether a microorganism is attenuated when a candidate virulence gene is inactivated. Thus, the use of animal models to identify the virulence factors of pathogens is indispensable. However, animal models are accompanied by ethical problems and cannot be used in large numbers for statistical analysis. Recently, nonmammalian models, including Caenorhabditis elegans, Drosophila melanogaster, Danio rerio, and larvae of the greater wax moth, Galleria mellonella, have been used for biological research (2-4). These organisms share many advantages over mammalian models, including being easy to obtain, cost-effective, and more ethically acceptable. However, the optimal temperature for C. elegans, D. melanogaster, and Danio rerio is below 28°C (5, 6). These models are defective since the optimum temperature for most mammal pathogens is 37°C. In contrast, the G. mellonella larva model is able to survive at 37°C. Moreover, the immune system of G. mellonella larvae

show abstract

Section: Discussionmentioning

confidence: 99%

“…To date, this infection model has been used to study the virulence factors, host response, and antimicrobial delivery of many pathogens (11)(12)(13). However, whether the G. mellonella larva model also can be used for the study of Riemerella anatipestifer infection remains unknown.…”

mentioning

confidence: 99%

New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept

Liu

Huang

et al. 2019

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Moreover, unlike C. elegans and D. melanogaster, the G. mellonella genome (GenBank NTHM00000000) is still a shotgun project that has not been fully analysed [99]. Also, few microarrays [100,101], libraries of RNA interference and mutant strains are available [6,102]. Recently, the analysis of host-pathogenic interactions at the molecular level using the G. mellonella model has shown many advances.…”

Section: Limitationmentioning

confidence: 99%

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

et al. 2019

View full text Add to dashboard Cite

A greater ethical conscience, new global rules and a modified perception of ethical consciousness entail a more rigorous control on utilizations of vertebrates for in vivo studies. To cope with this new scenario, numerous alternatives to rodents have been proposed. Among these, the greater wax moth Galleria mellonella had a preponderant role, especially in the microbiological field, as demonstrated by the growing number of recent scientific publications. The reasons for its success must be sought in its peculiar characteristics such as the innate immune response mechanisms and the ability to grow at a temperature of 37°C. This review aims to describe the most relevant features of G. mellonella in microbiology, highlighting the most recent and relevant research on antibacterial strategies, novel drug tests and toxicological studies. Although solutions for some limitations are required, G. mellonella has all the necessary host features to be a consolidated in vivo model host.

show abstract

“…These results of miRNA regulation for immune‐related gene following infection probe the evidence that host can activate a complete new pathway to address a new pattern of response for a secondary challenge with pathogen. Concerning chromatin structure pattern and the associate histone mark modification, or DNA methylation patterns few evidences exist concerning their potential involvement in innate immune response …”

Section: Toward a Unifying Model For Innate Immune Memorymentioning

confidence: 99%

Innate immune memory: An evolutionary perspective

Gourbal

Pinaud

Beckers

et al. 2018

Immunological Reviews

198

157

View full text Add to dashboard Cite

Over the last decades, there was increasing evidence for the presence of innate immune memory in living organisms. In this review, we compare the innate immune memory of various organisms with a focus on phylogenetics. We discuss the acquisition and molecular basis of immune memory and we describe the innate immune memory paradigm and its role in host defense during evolution. The molecular characterization of innate immunological memory in diverse organisms and host-parasite systems reconciles mechanisms with phenomena and paves the way to molecular comprehension of innate immune memory. We also revise the traditional classification of innate and adaptive immunity in jawed vertebrates. We emphasize that innate immune responses have the capacity to be "primed" or "trained", thereby exerting a yet unknown type of immunological memory upon re-infection.

show abstract

Epigenetic Mechanisms Regulate Innate Immunity against Uropathogenic and Commensal-Like Escherichia coli in the Surrogate Insect Model Galleria mellonella

Cited by 43 publications

References 50 publications

New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept

New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept

Galleria mellonella as a consolidated in vivo model hosts: New developments in antibacterial strategies and novel drug testing

Innate immune memory: An evolutionary perspective

Contact Info

Product

Resources

About