Epigenetic programming and reprogramming during development

Cantone, Irene; Fisher, Amanda G.

doi:10.1038/nsmb.2489

Cited by 395 publications

(295 citation statements)

References 125 publications

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“…Improving SCNT with Dnmt3l-KO donor cells specific therapeutic applications (Nishikawa et al 2008, Cantone & Fisher 2013, Long et al 2014. However, successful reprogramming occurs only in a proportion of cloned embryos; therefore, improvements in developmental efficiency and our understanding of epigenetic reprogramming are essential.…”

Section: Discussionmentioning

confidence: 99%

Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency

Liao¹,

Mo²,

Wu³

et al. 2015

Reproduction

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Nuclear transfer (NT) is a technique used to investigate the development and reprogramming potential of a single cell. DNA methyltransferase-3-like, which has been characterized as a repressive transcriptional regulator, is expressed in naturally fertilized egg and morula/blastocyst at pre-implantation stages. In this study, we demonstrate that the use of Dnmt3l-knockout (Dnmt3l-KO) donor cells in combination with Trichostatin A treatment improved the developmental efficiency and quality of the cloned embryos. Compared with the WT group, Dnmt3l-KO donor cell-derived cloned embryos exhibited increased cell numbers as well as restricted OCT4 expression in the inner cell mass (ICM) and silencing of transposable elements at the blastocyst stage. In addition, our results indicate that zygotic Dnmt3l is dispensable for cloned embryo development at pre-implantation stages. In Dnmt3l-KO mouse embryonic fibroblasts, we observed reduced nuclear localization of HDAC1, increased levels of the active histone mark H3K27ac and decreased accumulation of the repressive histone marks H3K27me3 and H3K9me3, suggesting that Dnmt3l-KO donor cells may offer a more permissive epigenetic state that is beneficial for NT reprogramming. Reproduction (2015) 150 245-256

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Section: Discussionmentioning

confidence: 99%

Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency

Liao¹,

Mo²,

Wu³

et al. 2015

Reproduction

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“…11 To begin to understand how adipogenic differentiation is epigenetically disrupted by dietinduced obesity, we turned our attention to understanding the epigenetic mechanisms that control physiologic adipogenic differentiation in lean mice. Our investigations have focused on the chromatin histone modifying enzyme HDAC9, whose dynamic pattern of expression during adipogenic differentiation stands out among all of the other members of the HDAC/histone acetyltransferase family of genes.…”

Section: Histone Deacetylase 9 (Hdac9) Is An Epigenetic Regulator Of mentioning

confidence: 99%

Role of histone deacetylase 9 in regulating adipogenic differentiation and high fat diet-induced metabolic disease

et al. 2014

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“…However, the emergence of genome-wide studies suggests that the role of DNA methylation may be dependent on its genomic context (Bird 2002;Jones 2012). In mammals, the most dramatic changes in global DNA methylation occur during embryonic development in a process known as epigenetic reprogramming (Cantone and Fisher 2013). The methylome of fully differentiated cells, however, is unique for each cell type and remarkably stable, whereby aberrant alterations are often associated with diseases such as cancer (Baylin and Jones 2011;Halley-Stott and Gurdon 2013).…”

mentioning

confidence: 99%

Reprogramming of the human intestinal epigenome by surgical tissue transposition

et al. 2014

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Extracellular cues play critical roles in the establishment of the epigenome during development and may also contribute to epigenetic perturbations found in disease states. The direct role of the local tissue environment on the post-development human epigenome, however, remains unclear due to limitations in studies of human subjects. Here, we use an isogenic human ileal neobladder surgical model and compare global DNA methylation levels of intestinal epithelial cells pre-and post-neobladder construction using the Infinium HumanMethylation450 BeadChip. Our study is the first to quantify the effect of environmental cues on the human epigenome and show that the local tissue environment directly modulates DNA methylation patterns in normal differentiated cells in vivo. In the neobladder, the intestinal epithelial cells lose their tissue-specific epigenetic landscape in a time-dependent manner following the tissue's exposure to a bladder environment. We find that de novo methylation of many intestine-specific enhancers occurs at the rate of 0.41% per month (P < 0.01, Pearson = 0.71), while demethylation of primarily non-intestine-specific transcribed regions occurs at the rate of -0.37% per month (P < 0.01, Pearson = -0.57). The dynamic resetting of the DNA methylome in the neobladder not only implicates local environmental cues in the shaping and maintenance of the epigenome but also illustrates an unexpected cross-talk between the epigenome and the cellular environment.

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Epigenetic programming and reprogramming during development

Cited by 395 publications

References 125 publications

Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency

Dnmt3l-knockout donor cells improve somatic cell nuclear transfer reprogramming efficiency

Role of histone deacetylase 9 in regulating adipogenic differentiation and high fat diet-induced metabolic disease

Reprogramming of the human intestinal epigenome by surgical tissue transposition

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