Epigenetic Regulation of Foetal Development in Nuclear Transfer Animal Models

Dinnyés, András; Tian, X.C.; Yang, Xiangzhong

doi:10.1111/j.1439-0531.2008.01178.x

Cited by 22 publications

(15 citation statements)

References 87 publications

(103 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies have examined cloned mouse and bovine embryos and identified epigenetic alterations in X chromosome inactivation and imprinting. Differences in DNA methylation both in general and of specific genes and repeat sequences have been reported [35]. One previous study observed similar demethylation activity in cloned and control pig embryos with respect to two repetitive sequences: a centromeric satellite and the PRE1 short interspersed element (SINE) [36].…”

Section: Discussionmentioning

confidence: 81%

Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls

Gao

Luo

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

Animal breeding via Somatic Cell Nuclear Transfer (SCNT) has enormous potential in agriculture and biomedicine. However, concerns about whether SCNT animals are as healthy or epigenetically normal as conventionally bred ones are raised as the efficiency of cloning by SCNT is much lower than natural breeding or In-vitro fertilization (IVF). Thus, we have conducted a genome-wide gene expression and DNA methylation profiling between phenotypically normal cloned pigs and control pigs in two tissues (muscle and liver), using Affymetrix Porcine expression array as well as modified methylation-specific digital karyotyping (MMSDK) and Solexa sequencing technology. Typical tissue-specific differences with respect to both gene expression and DNA methylation were observed in muscle and liver from cloned as well as control pigs. Gene expression profiles were highly similar between cloned pigs and controls, though a small set of genes showed altered expression. Cloned pigs presented a more different pattern of DNA methylation in unique sequences in both tissues. Especially a small set of genomic sites had different DNA methylation status with a trend towards slightly increased methylation levels in cloned pigs. Molecular network analysis of the genes that contained such differential methylation loci revealed a significant network related to tissue development. In conclusion, our study showed that phenotypically normal cloned pigs were highly similar with normal breeding pigs in their gene expression, but moderate alteration in DNA methylation aspects still exists, especially in certain unique genomic regions.

show abstract

Section: Discussionmentioning

confidence: 81%

Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls

Gao

Luo

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…It comes as no surprise that epigenetic modification of the genome is disrupted in nuclear transfer embryos; this phenomenon has been extensively reviewed elsewhere (3,18,51,84). It is somewhat surprising, however, that genes involved in epigenetic processes were overwhelmingly downregulated in embryos derived from SCNT.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies

Isom

Stevens

et al. 2013

Physiological Genomics

View full text Add to dashboard Cite

Isom SC, Stevens JR, Li R, Spollen WG, Cox L, Spate LD, Murphy CN, Prather RS. Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies. Physiol Genomics 45: 577-589, 2013. First published May 21, 2013 doi:10.1152/physiolgenomics.00094.2012.-Substantial mortality of in vitro manipulated porcine embryos is observed during peri-attachment development. Herein we describe our efforts to characterize the transcriptomes of embryonic disc (ED) and trophectoderm (TE) cells from porcine embryos derived from in vivo fertilization, in vitro fertilization (IVF), parthenogenetic oocyte activation (PA), and somatic cell nuclear transfer (SCNT) on days 10, 12, and 14 of gestation. The IVF, PA, and SCNT embryos were generated with in vitro matured oocytes and were cultured overnight in vitro before being transferred to recipient females. Sequencing of cDNA from the resulting embryonic samples was accomplished with the Genome Analyzer IIx platform from Illumina. Reads were aligned to a custom-built swine transcriptome. A generalized linear model was fit for ED and TE samples separately, accounting for embryo type, gestation day, and their interaction. Those genes with significant differences between embryo types were characterized in terms of gene ontologies and KEGG pathways. Transforming growth factor-␤ signaling was downregulated in the EDs of IVF embryos. In TE cells from IVF embryos, ubiquitin-mediated proteolysis and ErbB signaling were aberrantly regulated. Expression of genes involved in chromatin modification, gene silencing by RNA, and apoptosis was significantly disrupted in ED cells from SCNT embryos. In summary, we have used high-throughput sequencing technologies to compare gene expression profiles of various embryo types during peri-attachment development. We expect that these data will provide important insight into the root causes of (and possible opportunities for mitigation of) suboptimal development of embryos derived from assisted reproductive technologies.in vitro fertilization; parthenogenesis; somatic cell nuclear transfer; in vitro embryo production; Illumina; ART IN MOST MAMMALIAN SPECIES studied to date, only 50 -70% of successfully fertilized oocytes will give rise to live, healthy offspring (reviewed in Refs. 20,57,79). Some embryos succumb to the effects of chromosomal abnormalities or faulty uterine environments, for example. But the underlying cause behind most early embryonic mortality is still poorly understood. Such inefficiency is concerning to human fertility clinical practitioners and to animal producers in agricultural settings as well. Embryos produced by assisted reproductive technologies (ART) are generally less fit and more prone to failure than in vivo produced embryos. It has been presupposed that oocyte maturation and embryo culture systems that are inadequate to promote proper development are primarily to blame, yet the precise mechanisms that contribute to deficiencies in developmental potential in in vitr...

show abstract

“…Interest has dramatically increased as it has become clear that epigenetics will be essential to understanding many topical biological phenomena such as stem cells [14], nuclear transfer (cloning) [15], cellular reprogramming [16], aging [17], evolution and speciation [18], and agriculture [19].…”

Section: Relevance Of Epigenetics To Modern Medicinementioning

confidence: 99%

Epigenetic side-effects of common pharmaceuticals: A potential new field in medicine and pharmacology

2009

View full text Add to dashboard Cite

Epigenetic Regulation of Foetal Development in Nuclear Transfer Animal Models

Cited by 22 publications

References 87 publications

Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls

Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls

Transcriptional profiling by RNA-Seq of peri-attachment porcine embryos generated by a variety of assisted reproductive technologies

Epigenetic side-effects of common pharmaceuticals: A potential new field in medicine and pharmacology

Contact Info

Product

Resources

About