Epigenetic Regulation of miR‐29s Affects the Lactation Activity of Dairy Cow Mammary Epithelial Cells

Bian, Yanjie; Yu, Lei; Wang, Chunmei; Wang, Jie; Wang, Lina; Liu, Huan; Liu, Lixin; Gao, Xuejun; Qing-zhang, LI

doi:10.1002/jcp.24944

Cited by 80 publications

(61 citation statements)

References 44 publications

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“…Bian et al [122] showed that miRNA-29s regulate the DNA methylation level by inversely targeting both DNMT3A and DNMT3B in dairy cow MECs. The inhibition of miRNA-29s caused global DNA hypermethylation and increased the methylation levels of the promoters of important lactation-related genes, including casein-α s1 ( CSN1S1 ), E74-like factor 5 ( ELF5 ), PPARγ ( PPARG ), sterol regulatory element binding protein-1 ( SREBP1 ), and glucose transporter-1 ( GLUT1 ).…”

Section: Epigenetic Regulation Of Lactationmentioning

confidence: 99%

“…The inhibition of miRNA-29s reduced the secretion of lactoprotein, triacylglycerols and lactose by dairy cow MECs. The treatment of dairy cow MECs with 5-aza-2′-deoxycytidine decreased the methylation levels of the MIR29B promoter and increased the expression of miRNA-29b [122]. The enhancement of lactation activity and milk yield was thus associated with upregulation of DNMT3A- and DNMT3B-targeting miRNA-29s.…”

Section: Epigenetic Regulation Of Lactationmentioning

confidence: 99%

“…A key transcription factor of lipid synthesis is SREBP1, which is upregulated via activated AKT-mediated signal transduction. Notably, it has been demonstrated in bovine MECs that the expression of both AKT and SREBP1 underlie epigenetic miRNA-29/DNMT-mediated demethylation of their corresponding promoter regions [122]. Human milk contains and transfers milk stem cells with multilineage differentiation potential to the newborn infant [54,55].…”

Section: Intestinal Growthmentioning

confidence: 99%

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Milk’s Role as an Epigenetic Regulator in Health and Disease

2017

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Abstract:It is the intention of this review to characterize milk's role as an epigenetic regulator in health and disease. Based on translational research, we identify milk as a major epigenetic modulator of gene expression of the milk recipient. Milk is presented as an epigenetic "doping system" of mammalian development. Milk exosome-derived micro-ribonucleic acids (miRNAs) that target DNA methyltransferases are implicated to play the key role in the upregulation of developmental genes such as FTO, INS, and IGF1. In contrast to miRNA-deficient infant formula, breastfeeding via physiological miRNA transfer provides the appropriate signals for adequate epigenetic programming of the newborn infant. Whereas breastfeeding is restricted to the lactation period, continued consumption of cow's milk results in persistent epigenetic upregulation of genes critically involved in the development of diseases of civilization such as diabesity, neurodegeneration, and cancer. We hypothesize that the same miRNAs that epigenetically increase lactation, upregulate gene expression of the milk recipient via milk-derived miRNAs. It is of critical concern that persistent consumption of pasteurized cow's milk contaminates the human food chain with bovine miRNAs, that are identical to their human analogs. Commercial interest to enhance dairy lactation performance may further increase the epigenetic miRNA burden for the milk consumer.

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Section: Epigenetic Regulation Of Lactationmentioning

confidence: 99%

Section: Epigenetic Regulation Of Lactationmentioning

confidence: 99%

Section: Intestinal Growthmentioning

confidence: 99%

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Milk’s Role as an Epigenetic Regulator in Health and Disease

2017

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“…Among the likely more inhibited, the miRNA29b has strong epigenetic effects on the expression of several lactogenic genes [62], let-7a-5p is highly expressed in cow mammary [63], and miRNA1 has a strong control in the expression of β-lactoglobulin [64]. …”

Section: Resultsmentioning

confidence: 99%

Transcriptome difference and potential crosstalk between liver and mammary tissue in mid-lactation primiparous dairy cows

Bionaz

Wang

et al. 2017

PLoS ONE

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Liver and mammary gland are among the most important organs during lactation in dairy cows. With the purpose of understanding both the different and the complementary roles and the crosstalk of those two organs during lactation, a transcriptome analysis was performed on liver and mammary tissues of 10 primiparous dairy cows in mid-lactation. The analysis was performed using a 4×44K Bovine Agilent microarray chip. The transcriptome difference between the two tissues was analyzed using SAS JMP Genomics using ANOVA with a false discovery rate correction (FDR). The analysis uncovered >9,000 genes differentially expressed (DEG) between the two tissues with a FDR<0.001. The functional analysis of the DEG uncovered a larger metabolic (especially related to lipid) and inflammatory response capacity in liver compared with mammary tissue while the mammary tissue had a larger protein synthesis and secretion, proliferation/differentiation, signaling, and innate immune system capacity compared with the liver. A plethora of endogenous compounds, cytokines, and transcription factors were estimated to control the DEG between the two tissues. Compared with mammary tissue, the liver transcriptome appeared to be under control of a large array of ligand-dependent nuclear receptors and, among endogenous chemical, fatty acids and bacteria-derived compounds. Compared with liver, the transcriptome of the mammary tissue was potentially under control of a large number of growth factors and miRNA. The in silico crosstalk analysis between the two tissues revealed an overall large communication with a reciprocal control of lipid metabolism, innate immune system adaptation, and proliferation/differentiation. In summary the transcriptome analysis confirmed prior known differences between liver and mammary tissue, especially considering the indication of a larger metabolic activity in liver compared with the mammary tissue and the larger protein synthesis, communication, and proliferative capacity in mammary tissue compared with the liver. Relatively novel is the indication by the data that the transcriptome of the liver is highly regulated by dietary and bacteria-related compounds while the mammary transcriptome is more under control of hormones, growth factors, and miRNA. A large crosstalk between the two tissues with a reciprocal control of metabolism and innate immune-adaptation was indicated by the network analysis that allowed uncovering previously unknown crosstalk between liver and mammary tissue for several signaling molecules.

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“…MiR-486 regulation of lactation by targeting the PTEN gene in cow MGs has been demonstrated [87]. Bian et al [88] recently reported that epigenetic regulation of miR-29s affects the lactation activity of BMEC. MiR-181a was shown to regulate the biosynthesis of bovine milk fat through targeting acyl-CoA synthetase long-chain family member 1 (ACSL1) [89].…”

Section: Functional Validation Of Microrna Target Genesmentioning

confidence: 99%

Non-Coding RNA Roles in Ruminant Mammary Gland Development and Lactation

Ngoc¹,

Ibeagha‐Awemu²

2017

Current Topics in Lactation

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The ruminant mammary gland (MG) is an important organ charged with the production of milk for young and human nourishment. ) are a class of untranslated RNA molecules that function to regulate gene expression, associated biochemical pathways and cellular functions and are involved in many biological processes. This chapter presents a review of the current state of knowledge on the role of ncRNAs (particularly miRNAs and lncRNAs) in the MG and lactation processes, lactation signalling pathways, lipid metabolism, MG health of ruminants as well as miRNA roles in milk recipients. Finally, the potential application of new genome editing technology for ncRNA studies in MG development, the lactation process and milk components is presented.

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Epigenetic Regulation of miR‐29s Affects the Lactation Activity of Dairy Cow Mammary Epithelial Cells

Cited by 80 publications

References 44 publications

Milk’s Role as an Epigenetic Regulator in Health and Disease

Milk’s Role as an Epigenetic Regulator in Health and Disease

Transcriptome difference and potential crosstalk between liver and mammary tissue in mid-lactation primiparous dairy cows

Non-Coding RNA Roles in Ruminant Mammary Gland Development and Lactation

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