Epigenetic silencing of KLF2 by long non-coding RNA SNHG1 inhibits periodontal ligament stem cell osteogenesis differentiation

Abstract

Background Exploring the effects of lncRNA SNHG1 in the process of osteogenic differentiation of periodontal ligament stem cells (PDLSCs) would provide novel therapeutic strategies for tissue regeneration. Methods Loss-of-function and gain-of-function assays were induced by lentivirus. The osteogenic differentiation of PDLSCs were assessed by ALP staining and Alizarin Red staining as well as the mRNA and protein levels of osteogenic marker genes osterix, osteocalcin, and alkaline phosphatase through qRT-PCR and western blot. RNA immunoprecipitation assay and chromatin immunoprecipitation assays were performed to uncover the interaction between SNHG1 and EZH2. Results Our analysis revealed that SNHG1 was downregulated and KLF2 was upregulated during the osteogenic induction differentiation of PDLSCs. SNHG1 inhibited while KLF2 promoted osteogenic differentiation of PDLSCs. SNHG1 directly interact with the histone methyltransferase enhancer of the zeste homolog 2 (EZH2) and modulate the histone methylation of promoter of Kruppel-like factor 2 (KLF2) and altered the progress osteogenic differentiation of PDLSCs. Conclusions Taken together, SNHG1 inhibited the osteogenic differentiation of PDLSCs through EZH2-mediated H3K27me3 methylation of KLF2 promotor and provided a novel class of therapeutic targets for regenerate dental tissues.

Comprehensive Analysis of the Long Noncoding RNA‑associated Competitive Endogenous RNA Network in the Osteogenic Differentiation of Periodontal Ligament Stem Cells

“…In addition to these lncRNAs revealed in these paper, some classical lncRNAs, such as SNHG1, TUG1, GAS5, XIST, DANCR and FER1L4, were reported to be involved in the osteoblast differentiation of PDLSCs [17,[39][40][41][42][43], most of them function as ceRNAs (miRNA sponges) to regulate osteogenesis. In order to reveal a lncRNA-miRNA-mRNA network, a complete study includes procedures as follows.…”

“…The osteoblast differentiation of PDLSCs has an effect on epigenetic regulation, subsequently causing changes in gene expression [16][17][18]. Our previous study found that 2171 lncRNAs and 3557 messenger RNAs (mRNAs) were signi cantly differentially expressed during the osteoblast differentiation of PDLSCs.…”

Comprehensive analysis of the long noncoding RNA-associated competitive endogenous RNA network in the osteogenic differentiation of periodontal ligament stem cells

“…In addition to these lncRNAs revealed in these paper, some classical lncRNAs, such as SNHG1, TUG1, GAS5, XIST, DANCR and FER1L4, were reported to be involved in the osteogenic differentiation of PDLSCs [ 17 , 45 – 49 ], most of them function as ceRNAs (miRNA sponges) to regulate osteogenesis. In order to reveal a lncRNA-miRNA-mRNA network, a complete study includes procedures as follows.…”

Identification the Synergistic Effect of Immune-Related lncRNA KIAA0125 and Vitamin D Metabolism Related Gene CYP24A1 in Periodontitis

“…At present, some studies have con rmed that lncRNA may change the function of periodontal related cells in the microenvironment of periodontitis. Li et al Found lncRNA SNHG1 associated with osteoblastic dysfunction in periodontitis can regulate the osteogenic differentiation of in ammatory periodontal ligament stem cells through EZH2mediated H3K27me3 methylation of KLF2 promotor [21]. In this study, 4 DElncRNAs (KIAA0125, Runx1-IT1, LOC100130476, LOC101929272) were screened out from the dataset GSE10334.…”