Epigenetic therapeutics in dental pulp treatment: Hopes, challenges and concerns for the development of next-generation biomaterials

Duncan, Henry F.; Kobayashi, Y; Kearney, Michaela; Shimizu, Emi

doi:10.1016/j.bioactmat.2023.04.013

Cited by 10 publications

(9 citation statements)

References 305 publications

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“…The use of a global knockout in this study provided robust data using a validated model [20,43]; however, in the future, the potential use of a conditional KO model will increase the relevance of the research but may reduce the effect on the phenotype, as other supporting cells in the dentine-pulp complex are not affected. While further experimental work will be required to validate the effect of MMP13 ablation related specifically to the odontoblast cell, this study has, nevertheless, confirmed that there is an important role for MMP13 in stimulating mineralization processes in the injured pulp and highlighted an important novel target for the development of next-generation vital pulp treatments [44]. This study is potentially limited by the fact that it did not analyze the comparative injury response after pulpal exposure, focusing only on the response to injury in the absence of exposure.…”

Section: Discussionmentioning

confidence: 66%

The Reparative Function of MMP13 in Tertiary Reactionary Dentinogenesis after Tooth Injury

Duncan,

Kobayashi,

Yamauchi

et al. 2024

IJMS

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MMP13 gene expression increases up to 2000-fold in mineralizing dental pulp cells (DPCs), with research previously demonstrating that global MMP13 deletion resulted in critical alterations in the dentine phenotype, affecting dentine–tubule regularity, the odontoblast palisade, and significantly reducing the dentine volume. Global MMP13-KO and wild-type mice of a range of ages had their molar teeth injured to stimulate reactionary tertiary dentinogenesis. The response was measured qualitatively and quantitatively using histology, immunohistochemistry, micro-CT, and qRT-PCR in order to assess changes in the nature and volume of dentine deposited as well as mechanistic links. MMP13 loss affected the reactionary tertiary dentine quality and volume after cuspal injury and reduced Nestin expression in a non-exposure injury model, as well as mechanistic links between MMP13 and the Wnt-responsive gene Axin2. Acute pulpal injury and pulp exposure to oral fluids in mice teeth showed upregulation of the MMP13 in vivo, with an increase in the gene expression of Mmp8, Mmp9, and Mmp13 evident. These results indicate that MMP13 is involved in tertiary reactionary dentine formation after tooth injury in vivo, potentially acting as a key molecule in the dental pulp during dentine–pulp repair processes.

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Section: Discussionmentioning

confidence: 66%

The Reparative Function of MMP13 in Tertiary Reactionary Dentinogenesis after Tooth Injury

Duncan,

Kobayashi,

Yamauchi

et al. 2024

IJMS

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“…The results demonstrated that these materials supported 95% viable hDPSCs expressing positive mesenchymal and hematopoietic markers. Other studies have also demonstrated positive effects on dental pulp regeneration through the utilization of calcium-phosphate-based scaffolds [ [95] , [96] , [97] , [98] ]. However, in clinical applications, the use of a highly bioactive coating is often undesirable as it can potentially lead to instability of the implant or prosthesis.…”

Section: Discussionmentioning

confidence: 99%

Advances and challenges in regenerative dentistry: A systematic review of calcium phosphate and silicate-based materials on human dental pulp stem cells

Christie,

Musri,

Djustiana

et al. 2023

Materials Today Bio

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“…Several studies have shown that the response of DTSCs in bacterial inflammatory oral diseases is modulated by epigenetic mechanisms [75][76][77][78]. The most studied epigenetic mechanisms are DNA methylation, chemical modifications in histones, and non-coding RNAs.…”

Section: Emerging Players In Dtscs-lpsmentioning

confidence: 99%

Dental Stem Cells and Lipopolysaccharides: A Concise Review

Rodas-Junco,

Hernández-Solís,

Serralta-Interian

et al. 2024

IJMS

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Dental tissue stem cells (DTSCs) are well known for their multipotent capacity and regenerative potential. They also play an important role in the immune response of inflammatory processes derived from caries lesions, periodontitis, and gingivitis. These oral diseases are triggered by toxins known as lipopolysaccharides (LPS) produced by gram-negative bacteria. LPS present molecular patterns associated with pathogens and are recognized by Toll-like receptors (TLRs) in dental stem cells. In this review, we describe the effect of LPS on the biological behavior of DTSCs. We also focus on the molecular sensors, signaling pathways, and emerging players participating in the interaction of DTSCs with lipopolysaccharides. Although the scientific advances generated provide an understanding of the immunomodulatory potential of DTSCs, there are still new reflections to explore with regard to their clinical application in the treatment of oral inflammatory diseases.

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Epigenetic therapeutics in dental pulp treatment: Hopes, challenges and concerns for the development of next-generation biomaterials

Cited by 10 publications

References 305 publications

The Reparative Function of MMP13 in Tertiary Reactionary Dentinogenesis after Tooth Injury

The Reparative Function of MMP13 in Tertiary Reactionary Dentinogenesis after Tooth Injury

Advances and challenges in regenerative dentistry: A systematic review of calcium phosphate and silicate-based materials on human dental pulp stem cells

Dental Stem Cells and Lipopolysaccharides: A Concise Review

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