Epigenetics and vitrification of embryos: one step forward and many raised equivocal questions

Blumenfeld, Zeev

doi:10.1016/j.fertnstert.2012.04.031

Cited by 1 publication

(1 citation statement)

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“…It was reported that vitrification weakened mitochondrial functions because of increased levels of reactive oxygen species (ROS) in mouse MII oocytes, and this may finally affect the development of subsequent embryos (Amoushahi, Salehnia, & Mowla, ). Besides, recent investigations revealed that oocyte and embryo cryopreservation are frequently accompanied by epigenetic alterations (Blumenfeld, ; Urrego, Rodriguez‐Osorio, Niemann, ). According to our previous study, DNA methylation, histone modification, and expression of some imprinted genes were sensitive to oocyte vitrification in cattle (Chen et al, ; Chen et al, ).…”

Section: Introductionmentioning

confidence: 99%

Resveratrol improved the developmental potential of oocytes after vitrification by modifying the epigenetics

Chen

Zhang

Wang

et al. 2019

Molecular Reproduction Devel

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Resveratrol (Res) has been reported to be able to improve oocyte vitrification because of its antioxidative properties. The objective of this study was to further assess the positive effect of Res addition on the developmental potential of vitrified mouse oocytes from the perspective of epigenetic alterations. First, 2 μM Res was chosen as the optimal concentration on the basis of its effects on survival and its antioxidative properties. We found that Res addition significantly promoted fertilization (63.8% vs. 42.9%) and blastocyst formation (68.3% vs. 50.2%) after oocyte vitrification. The quality of the derived blastocysts was also higher after Res treatment. Regarding epigenetic aspects, the expression of the important deacetylase SIRT1 was found to decrease significantly upon vitrification, but it was rescued by Res. The abnormal levels of H3K9 acetylation and DNA methylation in vitrified oocytes were restored by Res addition. Moreover, the expression of several imprinted genes was affected by oocyte vitrification. Among them, abnormal Gtl2 and Peg3 expression levels were restored by Res addition. Therefore, the methylation of their imprinted control regions (ICRs) was examined. Surprisingly, the abnormal patterns of Gtl2 and Peg3 methylation in blastocysts developed from vitrified oocytes were both restored by Res addition. Finally, the full‐term embryonic development showed that the birth rate was improved significantly by Res addition (56.2% vs. 38.1%). Collectively, Res was beneficial for the pre‐ and postimplantation embryonic development. Except for the antioxidative activity, Res also played a role in the correction of some abnormal epigenetic modifications caused by oocyte vitrification.

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Section: Introductionmentioning

confidence: 99%