Epigenomic profiling of mouse nucleus accumbens at single-cell resolution

Bhatia, Parth; Yang, Lanbo; Luo, Jay X. J.; Xu, Mengyi; Renthal, William

doi:10.1016/j.mcn.2023.103857

Cited by 3 publications

(2 citation statements)

References 40 publications

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“…scRNA-seq has been shown to be an effective tool for characterizing cellular diversity in brain regions associated with the reward system, such as the NAc and VTA [54,55]. The NAc is a brain region primarily composed of two main types of cells, namely, Medium Spiny Neurons (MSNs) and interneurons.…”

Section: Identifying Cell Populations By Molecular Clusteringmentioning

confidence: 99%

“…Using this approach, in the NAc, novel subpopulations of interneurons and MSNs were identified [52]. scATAC-seq has been used to map cell-type-specific differences in chromatin accessibility in the NAc, providing insights into the epigenomic landscape of this brain region [55]. Moreover, the characterization of γ-Aminobutyric Acid (GABA) MSNs and the discovery of notable variations in receptor expression patterns and MSN activation within NAc subterritories have highlighted the anatomical and functional heterogeneity of the NAc [56].…”

Section: Identifying Cell Populations By Molecular Clusteringmentioning

confidence: 99%

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Breaking the Chains: Advances in Substance Addiction Research through Single-Cell Sequencing, Epigenetics, and Epitranscriptomic

Filošević Vujnović,

Stanković Matić,

Saftić Martinović

et al. 2024

Future Pharmacology

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Addiction is a complex brain disease influenced by genetic, environmental, and neurological factors. Psychostimulants, cocaine, and methamphetamine influence different cell types in different brain regions, with a focus on the neurons responsible for rewarding effects in the nucleus accumbens (NAc) and ventral tegmental area (VTA). Known markers for psychostimulant-induced neuronal plasticity in combination with droplet-based high-throughput single-cell sequencing divided the heterogeneity of cell populations in NAc and VTA into clusters, where all cells of the same type do not respond equally to exposure to psychostimulants. To explain psychostimulant-induced neuronal plasticity as changes in the amplitude and phase shifts of gene expression, we focused on epigenetic mechanisms of DNA and chromatin modifications, as well as DNA accessibility. We also comment on epitranscriptomics as a novel approach in the study of messenger RNA posttranslational modification, which regulates translation and potentially localized transcription in synapses in order to address the molecular chains that connect addiction from changes in gene expression to synaptic and, finally, neuronal plasticity.

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Section: Identifying Cell Populations By Molecular Clusteringmentioning

confidence: 99%

Section: Identifying Cell Populations By Molecular Clusteringmentioning

confidence: 99%

Breaking the Chains: Advances in Substance Addiction Research through Single-Cell Sequencing, Epigenetics, and Epitranscriptomic

Filošević Vujnović,

Stanković Matić,

Saftić Martinović

et al. 2024

Future Pharmacology

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Genetics and epigenetics approaches as a path to the future of addiction science

West,

Day

2023

Molecular and Cellular Neuroscience

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The single-cell opioid responses in the context of HIV (SCORCH) consortium

Ament,

Campbell,

Lobo

et al. 2024

Mol Psychiatry

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Substance use disorders (SUD) and drug addiction are major threats to public health, impacting not only the millions of individuals struggling with SUD, but also surrounding families and communities. One of the seminal challenges in treating and studying addiction in human populations is the high prevalence of co-morbid conditions, including an increased risk of contracting a human immunodeficiency virus (HIV) infection. Of the ~15 million people who inject drugs globally, 17% are persons with HIV. Conversely, HIV is a risk factor for SUD because chronic pain syndromes, often encountered in persons with HIV, can lead to an increased use of opioid pain medications that in turn can increase the risk for opioid addiction. We hypothesize that SUD and HIV exert shared effects on brain cell types, including adaptations related to neuroplasticity, neurodegeneration, and neuroinflammation. Basic research is needed to refine our understanding of these affected cell types and adaptations. Studying the effects of SUD in the context of HIV at the single-cell level represents a compelling strategy to understand the reciprocal interactions among both conditions, made feasible by the availability of large, extensively-phenotyped human brain tissue collections that have been amassed by the Neuro-HIV research community. In addition, sophisticated animal models that have been developed for both conditions provide a means to precisely evaluate specific exposures and stages of disease. We propose that single-cell genomics is a uniquely powerful technology to characterize the effects of SUD and HIV in the brain, integrating data from human cohorts and animal models. We have formed the Single-Cell Opioid Responses in the Context of HIV (SCORCH) consortium to carry out this strategy.

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Epigenomic profiling of mouse nucleus accumbens at single-cell resolution

Cited by 3 publications

References 40 publications

Breaking the Chains: Advances in Substance Addiction Research through Single-Cell Sequencing, Epigenetics, and Epitranscriptomic

Breaking the Chains: Advances in Substance Addiction Research through Single-Cell Sequencing, Epigenetics, and Epitranscriptomic

Genetics and epigenetics approaches as a path to the future of addiction science

The single-cell opioid responses in the context of HIV (SCORCH) consortium

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