Epilobium angustifolium L. extract with high content in oenothein B on benign prostatic hyperplasia: A monocentric, randomized, double-blind, placebo-controlled clinical trial

Esposito, Cristina; Santarcangelo, Cristina; Masselli, Raffaello; Buonomo, Giuseppe; Nicotra, Giovanna; Insolia, Violetta; D'Avino, Maria; Caruso, Giuseppe; Buonomo, Antonio Riccardo; Sacchi, Roberto; Sommella, Eduardo; Campiglia, Pietro; Tenore, Gian Carlo; Daglia, Maria

doi:10.1016/j.biopha.2021.111414

Cited by 17 publications

(16 citation statements)

References 27 publications

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“…Oenothein B is a main bioactive compound with therapeutic potential in BPH due to complex mechanism of action involving the regulation of androgen levels, inhibition of prostate-specific antigen (PSA) as well as anti-proliferative and apoptotic properties (Hiermann et al 1998;Vitalone et al 2001;Stolarczyk et al 2013). The results of clinical trial (Esposito et al 2021) and numerous preclinical studies indicated that Ch. angustifolium extracts can be used as drugs or food supplements.…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Dreger

Seidler-Łożykowska

Szalata

et al. 2021

Plant Cell Tiss Organ Cult

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The purpose of the study was to evaluate Chamerion angustifolium (L.) Holub genotypes for preliminary selection and further breeding programs aimed at obtaining a suitable industrial form for the pharmaceutical applications. Clonally propagated plants representing 10 genotypes of Ch. angustifolium were regenerated under in vitro conditions, hardened and planted in the field. Studies included an evaluation of shoot proliferation, phytochemical assessment of in vitro and ex vitro plants as well as investigations of intraspecies variability regarding four phenological stages: vegetative, beginning of blooming, full blooming, and green fruit phases. Quantitative and qualitative analyses of bioactive compounds were performed using high-performance liquid chromatography coupled with diode array detector and tandem mass spectrometer (HPLC–DAD–MS/MS) and high-performance liquid chromatography (HPLC) methods. The efficiency of shoot multiplication varied between genotypes from 8.12 to 21.48 shoots per explant. A high reproduction rate (> 20 shoots per explant) was recorded for four lines (PL_45, PL_44, PL_58, DE_2). Plants grown in vitro synthesized oenothein B (11.2–22.3 mg g−1 DW) and caffeic acid derivatives. Plants harvested from field contained the full spectrum of polyphenols characteristic for this species, and oenothein B and quercetin 3-O-glucuronide were the most abundant. The maximal content of oenothein B was determined in the vegetative phase of fireweed, while some flavonoids were found in the highest amount in full blooming phase. The results of analysis of variance indicated significant differences among genotypes in oenothein B, 3-O-caffeoylquinic acid and flavonoids accumulation in four phenological phases. PL_44 plants were characterized by high content of oenothein B and quercetin 3-O-glucuronide as well as a relatively high level of other flavonoids. Based on our phytochemical and micropropagation studies, PL_44 genotype was the best candidate for early selection and further breeding programs.

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Section: Discussionmentioning

confidence: 99%

“…angustifolium extracts have a positive effect on BPH (Vitalone and Allkanjari 2018;Deng et al 2019). Recently published results of a double-blind, placebo-controlled clinical trial confirmed beneficial effect of fireweed extract in alleviating BPH symptoms (Esposito et al 2021).…”

Section: Introductionmentioning

confidence: 92%

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Dreger

Seidler-Łożykowska

Szalata

et al. 2021

Plant Cell Tiss Organ Cult

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“…In addition, the young leaves of the Epilobium species are often used as food and feed [ 11 , 15 ]. To confirm the ethnobotanical uses of the species, several pharmacological and biochemical studies on the members of the genus have been performed [ 13 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. In addition to these pharmacological studies, the presence of biologically active compounds including tannins (especially ellagitannins), myricetin, quercetin, and several phenolic acids have been reported in phytochemical studies [ 18 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Shedding Light into the Connection between Chemical Components and Biological Effects of Extracts from Epilobium hirsutum: Is It a Potent Source of Bioactive Agents from Natural Treasure?

Zengin

Mahomoodally

et al. 2021

Antioxidants

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Epilobium hirsutum is extensively used as a traditional remedy in folk medicine, especially against prostate inflammation. Therefore, we evaluated the chemical profiles and biopharmaceutical potentials of different extracts of E. hirsutum aerial parts and roots. Metabolomic, antioxidant, and enzyme inhibitory profiles were investigated. Human prostate cancer PC3 cells were exposed to the extracts to evaluate antiproliferative effects. Gene expression and bioinformatics analyses were performed to investigate anti-inflammatory mechanisms. Oenothein B and myricetin were prominent compounds in the extracts. In scavenging/reducing assays, the methanol, infusion, and methanol/water extracts exhibited similar activities. We also observed the reduction of PC3 viability occurring following exposure to methanol and methanol/water extracts. According to bioinformatics analysis, myricetin was predicted to interact with COX-2 and TNFα. The interaction between TNFα and oxo-dihydroxy-octadecenoic acid was predicted as well. Intriguingly, the gene expression of COX-2 and TNFα was reduced in PC3 cells after exposure to methanol and methanol/water extracts. These effects were paralleled by the decreased gene expression of IL-8 and NFkB and the inhibition of PGE2 release. Therefore, the present findings suggest the potential use of E. hirsutum for the management of the burden of inflammation and oxidative stress occurring in lower urinary tract diseases, including prostatitis.

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“…Apart from urinary disease, the willowherb is traditionally used to treat wounds and skin diseases, pain (migraine headaches, childbirth, stomach ache, sore throat), menstrual disorders, gastrointestinal disorders, insomnia, fever (Granica et al, 2014;Schepetkin et al, 2016;Shikov et al, 2006;Sõukand et al, 2020). The justification for its use to reduce nocturia in subjects with benign prostatic hyperplasia was confirmed by clinical trial (Esposito et al, 2021). The main pharmacologically active ingredients of willowherb extracts are polyphenols including phenolic acids, flavonoids and ellagitannins.…”

Section: Introductionmentioning

confidence: 99%

Effect of carrier type on the spray-dried willowherb (Epilobium angustifolium L.) leaves extract, powder properties and bioactive compounds encapsulation

Jovanović¹,

Drinić²,

Bigović³

et al. 2021

Lekovite sirovine

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Willowherb (Epilobium angustifolium L.) leaves are a valuable source of polyphenolic compounds (phenolic acids, flavonoids, and ellagitannins) that are mainly used in the treatment of benign prostatic hyperplasia. In the presented study, the impacts of maltodextrin and whey protein as carriers on the efficiency of spray drying and physicochemical properties of the obtained powders were examined. The use of carriers significantly improved the drying yield (over 60 %). Moisture content, hygroscopicity, and rehydration time of all dried extracts (without the carrier, with maltodextrin and whey protein) were at an acceptable level, while their flowability and cohesiveness were poor. The encapsulation efficiency of polyphenols in a whey protein carrier of 92.02 % was significantly higher compared to 75.80 % for maltodextrin. These results show that whey protein efficiently encloses the extract ingredients into powder particles and thus preserves sensitive phenolic compounds during the drying process. The efficacy of flavonoid encapsulation was also high (93.00 % for maltodextrin and 94.34 % for whey protein), with no statistically significant differences between carrier types. Therefore, willowherb leaves extract can be successfully encapsulated by spray drying using maltodextrin and whey protein as carriers.

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Epilobium angustifolium L. extract with high content in oenothein B on benign prostatic hyperplasia: A monocentric, randomized, double-blind, placebo-controlled clinical trial

Cited by 17 publications

References 27 publications

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Shedding Light into the Connection between Chemical Components and Biological Effects of Extracts from Epilobium hirsutum: Is It a Potent Source of Bioactive Agents from Natural Treasure?

Effect of carrier type on the spray-dried willowherb (Epilobium angustifolium L.) leaves extract, powder properties and bioactive compounds encapsulation

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