Epinephrine and dopamine colocalization with norepinephrine in various peripheral tissues: Guanethidine effects

Villanueva, Iván; Piñón, Manuel; Quevedo-Corona, Lucı́a; Martı́nez-Olivares, Rubén; Racotta, Radu

doi:10.1016/s0024-3205(03)00491-0

Cited by 19 publications

(10 citation statements)

References 26 publications

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“…Average norepinephrine content remaining in these tissues of guanethidine-treated rats ranged from 15 to 39% of control values; however, the norepinephrine content of the liver was not affected by prior guanethidine treatment. This contrasts with the findings of Villanueva et al (2003) who reported that guanethidine-treated rats have less than 3% of the liver norepinephrine content of control animals. This difference is most likely accounted for by either differences in the age of the animals when guanethidine was first introduced or in the route of administration or both.…”

Section: Discussioncontrasting

confidence: 99%

Guanethidine treatment does not block the ability of central leptin administration to decrease blood glucose concentrations in streptozotocin-induced diabetic rats

Wang

Wernette²,

Judd³

et al. 2008

Journal of Endocrinology

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Leptin, administered either into the ventricles of the brain or systemically, has been shown to normalize blood glucose concentrations in streptozotocin (STZ)-induced diabetic rats. We hypothesized that an intact sympathetic nervous system is necessary for centrally administered leptin to normalize or attenuate high blood glucose concentrations in STZ-induced diabetic rats. Young male Wistar rats (w50 g) were treated every other day with either s.c. guanethidine (100 mg/kg) or vehicle for 2 weeks. Rats were then implanted with an intracerebroventricular cannula directed to the lateral ventricle and made diabetic with an i.v. injection of STZ (50 mg/kg). Half of the animals in each group were given daily injections of leptin (10 ml), while the remaining animals received vehicle injections. Blood glucose concentrations were measured daily and tissue norepinephrine content was determined by high performance liquid chromatography at the end of the study. Guanethidine pretreatment did not block the ability of centrally administered leptin to decrease blood glucose concentrations in diabetic rats. This suggests that the sympathetic nervous system does not mediate the leptininduced attenuation of high blood glucose concentrations observed in diabetic rats.

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Section: Discussioncontrasting

confidence: 99%

Guanethidine treatment does not block the ability of central leptin administration to decrease blood glucose concentrations in streptozotocin-induced diabetic rats

Wang

Wernette²,

Judd³

et al. 2008

Journal of Endocrinology

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“…CTRL: control, Gua: sympathectomy group, Los: losartan-treated group, Gua + Los: combined treatment group. Guanethidine induces a dramatic loss of catecholamine in the circulation and all tissues (Aberdeen et al, 1990, Villanueva et al, 2003 and is highly selective for sympathetic fibers. We choose guanethidine from other substances used for sympathectomy such as 6-hydroxydopamine, because its destructive effect is more efficient in the rat (Johnson, 1975).…”

Section: Discussionmentioning

confidence: 99%

Regulation of aortic extracellular matrix synthesis via noradrenergic system and angiotensin II in juvenile rats

Dab¹,

Hachani²,

Dhaouadi³

et al. 2012

Pharmaceutical Biology

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“…Chronic oral administration of guanethidine in ducklings has been reported to produce an efficient sympathectomy (Filali-Zegzouti et al 2005), as previously shown in rats following intraperitoneal or subcutaneous infusion (Romanyshyn and Clarke 1976;Picklo 1997;Villanueva et al 2003). By using guanethidine, we aimed to investigate the possibility that the sympathetic nervous system might modulate mitochondrial respiratory properties of heart and skeletal muscles in ducklings.…”

Section: Introductionmentioning

confidence: 91%

“…In terms of the source of circulating catecholamines in birds, Rome and Bell (1983) have shown that norepinephrine alone constitutes the predominant sympathetic neurotransmitter in fowl. In rats, Villanueva et al (2003) have shown that chemical sympathectomy with guanethidine selectively blocks the release of norepinephrine from the postganglionic nerve terminals, leading to a decrease in norepinephrine concentrations in several tissues, including skeletal muscles (Navegantes et al 1999;Villanueva et al 2003). Similarly, chronic guanethidine treatment leads to a fifty percent drop in norepinephrine concentrations in the heart and skeletal muscles of ducklings (Filali-Zegzouti et al 2000).…”

Section: Control Guanethidinementioning

confidence: 99%

Mitochondrial oxidative energy metabolism in guanethidine-induced sympathectomized ducklings

Filali-Zegzouti

Rouanet²,

Fechtali³

et al. 2014

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Abstract.Here we investigate the possible involvement of the sympathetic nervous system in the respiratory properties of intermyofibrillar and subsarcolemmal mitochondrial populations from heart and gastrocnemius muscles. Mitochondrial oxidative phosphorylation was assessed polarographically by using succinate (plus rotenone), and ascorbate plus N,N,N' ,N'-tetramethyl-p-phenyl-enediamine (plus antimycin) as respiratory substrates. We report that chronic chemical sympathectomy with guanethidine (150 mg/kg, daily for 3 weeks) induced a marked decrease in whole body metabolic and heart rates, in plasma metabolites (fatty acids and glucose) and norepinephrine levels. Guanethidine treatment decreased mainly the oxidative phosphorylation capacity of subsarcolemmal mitochondria in heart, irrespective of the substrate used. In contrast, both mitochondrial populations were affected by the treatment in skeletal muscle. This suggests that sympathetic nervous system activity can alter the energetic status of muscle cells, and to some extent play a thermogenic role in birds.

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Epinephrine and dopamine colocalization with norepinephrine in various peripheral tissues: Guanethidine effects

Cited by 19 publications

References 26 publications

Guanethidine treatment does not block the ability of central leptin administration to decrease blood glucose concentrations in streptozotocin-induced diabetic rats

Guanethidine treatment does not block the ability of central leptin administration to decrease blood glucose concentrations in streptozotocin-induced diabetic rats

Regulation of aortic extracellular matrix synthesis via noradrenergic system and angiotensin II in juvenile rats

Mitochondrial oxidative energy metabolism in guanethidine-induced sympathectomized ducklings

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