Epiphytic and endophytic microorganisms associated to different cultivar of tomato fruits in greenhouse environment and characterization of beneficial bacterial strains for the control of post-harvest tomato pathogens

Panebianco, S.; Lombardo, Monia Federica; Anzalone, Alice; Musumarra, A.; Catara, Vittoria; Cirvilleri, G.

doi:10.1016/j.ijfoodmicro.2022.109861

Cited by 10 publications

(9 citation statements)

References 39 publications

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“…In order to proceed to a data rejection for A1–A4 samples, we infer that the high yields of Br found in the samples from the greenhouse n. 1 could be related to anthropogenic causes (specific soil treatment, use of particular pesticides before harvesting, etc.). This conclusion can be deduced also by our previous study concerning the epiphytic and endophytic microbial populations isolated from the samples A1–A4 19 . These samples, although coming from farm 1 in the Pachino area, separated from the other samples creating a third cluster.…”

Section: Xrf Measurements and Analysissupporting

confidence: 78%

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XRF analysis searching for fingerprint elemental profile in south-eastern Sicily tomatoes

Panebianco,

Pellegriti,

Finocchiaro

et al. 2023

Sci Rep

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The implementation of analytical techniques able to certify food quality and origin in a fast and non-destructive way is becoming a widespread need in the agri-food sector. Among the physical non-destructive techniques, X-ray fluorescence (XRF) spectrometry is often used to analyze the elemental composition of biological samples. In this study, X-ray fluorescence (XRF) elemental profiles were measured on tomato samples belonging to different geographical areas in Sicily (Italy). The purpose of this investigation was aiming to establish a protocol for in-situ measurement and analysis able to provide quality assessment and traceability of PGI agri-food products, specifically sustaining health safety and self qualifying bio-chemical signature. In detail, sampling was performed in one of the most tomato productive area of south-eastern Sicily (Pachino district), characterised by a relative higher amount of Organic Carbon and Cation Exchange Capacity, and compared with samples from other growing areas of Sicily, falling in Ragusa province and Mt. Etna region. Experimental data were analyzed in the framework of multivariate analysis by using principal component analysis and further validated by discriminant analysis. The results show the presence of specific elemental signatures associated to several characterizing elements. This methodology establishes the possibility to disentangle a clear fingerprint pattern associated to the geographical origin of an agri-food product.

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Section: Xrf Measurements and Analysissupporting

confidence: 78%

“…Microbiological analyses were also performed on tomato samples harvested in the Pachino district during the same campaign 19 .…”

Section: Area Of Origin and Sample Characteristicsmentioning

confidence: 99%

XRF analysis searching for fingerprint elemental profile in south-eastern Sicily tomatoes

Panebianco,

Pellegriti,

Finocchiaro

et al. 2023

Sci Rep

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“…In the range of isolated microbes, the genera most closely related to disease resistance were Enterobacter (bacteria) and Talaromyces (fungi). Enterobacter can improve host disease resistance, promote plant growth, and enhance abiotic stress resistance ( Ajmal et al., 2022 ; Mukherjee et al., 2022 ; Panebianco et al., 2022 ). Talaromyces is ubiquitously used as biocontrol agents worldwide ( Thambugala et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Rhizosphere microorganisms of Crocus sativus as antagonists against pathogenic Fusarium oxysporum

Zhang

Zhu

et al. 2022

Front. Plant Sci.

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IntroductionSeveral microorganisms in the plant root system, especially in the rhizosphere, have their own compositions and functions. Corm rot is the most severe disease of Crocus sativus, leading to more than 50% mortality in field production.MethodsIn this study, metagenomic sequencing was used to analyze microbial composition and function in the rhizosphere of C. sativus for possible microbial antagonists against pathogenic Fusarium oxysporum.ResultsThe microbial diversity and composition were different in the C. sativus rhizosphere from different habitats. The diversity index (Simpson index) was significantly lower in the C. sativus rhizospheric soil from Chongming (Rs_CM) and degenerative C. sativus rhizospheric soil from Chongming (RsD_CM) than in others. Linear discriminant analysis effect size results showed that differences among habitats were mainly at the order (Burkholderiales, Micrococcales, and Hypocreales) and genus (Oidiodendron and Marssonina) levels. Correlation analysis of the relative lesion area of corm rot showed that Asanoa was the most negatively correlated bacterial genus (ρ = −0.7934, p< 0.001), whereas Moniliophthora was the most negatively correlated fungal genus (ρ = −0.7047, p< 0.001). The relative lesion area result showed that C. sativus from Qiaocheng had the highest resistance, followed by Xiuzhou and Jiande. C. sativus groups with high disease resistance had abundant pathogen resistance genes, such as chitinase and β-1,3-glucanase genes, from rhizosphere microorganisms. Further, 13 bacteria and 19 fungi were isolated from C. sativus rhizosphere soils, and antagonistic activity against pathogenic F. oxysporum was observed on potato dextrose agar medium. In vivo corm experiments confirmed that Trichoderma yunnanense SR38, Talaromyces sp. SR55, Burkholderia gladioli SR379, and Enterobacter sp. SR343 displayed biocontrol activity against corm rot disease, with biocontrol efficiency of 20.26%, 31.37%, 39.22%, and 14.38%, respectively.DiscussionThis study uncovers the differences in the microbial community of rhizosphere soil of C. sativus with different corm rot disease resistance and reveals the role of four rhizospheric microorganisms in providing the host C. sativus with resistance against corm rot. The obtained biocontrol microorganisms can also be used for application research and field management.

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“…lycopersici. Other epiphytic/endophytic microbes commonly found in/on tomatoes were seen in both the healthy and inoculated samples (Dong et al, 2019;Panebianco et al, 2022). In addition, it is likely that the unclassified reads are plant sequences.…”

Section: Downstream Nanopore Sequencing Can Identify Phytopathogens I...mentioning

confidence: 99%

Advancing the automation of plant nucleic acid extraction for rapid diagnosis of plant diseases in space

Haveman

Schuerger

et al. 2023

Front. Plant Sci.

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Human space exploration missions will continue the development of sustainable plant cultivation in what are obviously novel habitat settings. Effective pathology mitigation strategies are needed to cope with plant disease outbreaks in any space-based plant growth system. However, few technologies currently exist for space-based diagnosis of plant pathogens. Therefore, we developed a method of extracting plant nucleic acid that will facilitate the rapid diagnosis of plant diseases for future spaceflight applications. The microHomogenizer™ from Claremont BioSolutions, originally designed for bacterial and animal tissue samples, was evaluated for plant–microbial nucleic acid extractions. The microHomogenizer™ is an appealing device in that it provides automation and containment capabilities that would be required in spaceflight applications. Three different plant pathosystems were used to assess the versatility of the extraction process. Tomato, lettuce, and pepper plants were respectively inoculated with a fungal plant pathogen, an oomycete pathogen, and a plant viral pathogen. The microHomogenizer™, along with the developed protocols, proved to be an effective mechanism for producing DNA from all three pathosystems, in that PCR and sequencing of the resulting samples demonstrated clear DNA-based diagnoses. Thus, this investigation advances the efforts to automate nucleic acid extraction for future plant disease diagnosis in space.

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Epiphytic and endophytic microorganisms associated to different cultivar of tomato fruits in greenhouse environment and characterization of beneficial bacterial strains for the control of post-harvest tomato pathogens

Cited by 10 publications

References 39 publications

XRF analysis searching for fingerprint elemental profile in south-eastern Sicily tomatoes

XRF analysis searching for fingerprint elemental profile in south-eastern Sicily tomatoes

Rhizosphere microorganisms of Crocus sativus as antagonists against pathogenic Fusarium oxysporum

Advancing the automation of plant nucleic acid extraction for rapid diagnosis of plant diseases in space

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