Epiphytic Bacteria of Macroalgae of the Genus Ulva and their Potential in Producing Enzymes Having Biotechnological Interest

Nb, Comba-Gonzalez; Toquica, Jordan Steven Ruiz; López‐Kleine, Liliana; Montoya-Castaño, Dolly

doi:10.4172/2324-8661.1000153

Cited by 9 publications

(11 citation statements)

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“…These compounds can be degraded by enzymes that are synthesized by epiphytic bacteria [5]. For this reason, algal surfaces are an important source of a variety of enzymes [36,57]. Different methodologies have been used for screening enzymes of bacterial origin.…”

Section: Discussionmentioning

confidence: 99%

“…(LipA) [35] and Haliclona simulans (Lpc53E1) [95], and recently, in the epiphytic bacterium Shewanella algae from the brown macroalgae Ascophyllum nodosum [96]. Although, knowledge of lipases from green macroalgae epiphytic bacteria is limited [57,97], the efforts to describe and characterize marine lipases from this source have actually shown that cultivable epiphytic bacteria from U. lactuca are capable of producing lipases and other hydrolytic enzymes [1].…”

Section: Identification Of a "True" Lipasementioning

confidence: 99%

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Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

2020

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Epiphytic bacteria from marine macroalgae synthesize enzymes of industrial and biotechnological interest. In this study, we obtained two DNA candidate fragments for lipid-degrading enzymes from the total DNA of Ulva lactuca-associated epiphytic bacteria. First, we evaluated a method for total bacterial DNA isolation from the surface of U. lactuca thalli. Then, we designed sets of primers and used them directly for PCR amplification. The resulting PCR products were sequence-analyzed and used for expression and functional evaluation with the Escherichia coli pBAD-TOPO system. We obtained high molecular weight and good quality total bacterial DNA that served as a template to identify a fragment corresponding to an Acetyl-CoA C-Acetyltransferase (or Thiolase), and a candidate fragment for a versatile “true” lipase. We expressed the possible “true” lipase gene fragment heterologously in Escherichia coli and obtained proof of hydrolytic activity on Tributyrin, Tween-20, and Olive-oil media. This study resulted in new knowledge on U. lactuca-associated epiphytic bacteria as possible brand-new sources of enzymes such as thiolases and “true” lipases. However, future studies are required to describe the characteristics and important applications of these candidate enzymes.

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Section: Discussionmentioning

confidence: 99%

Section: Identification Of a "True" Lipasementioning

confidence: 99%

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

2020

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“…Enzymes produced by marine microorganisms can provide numerous advantages compared to traditional enzymes due to their wide-ranging habitats. The quest for bacterial isolates producing enzymes with high efficacy and of commercial value is still ongoing to exploit enormous marine resources, one of which are marine macroalgae and bacteria associated with their surfaces, seaweedsepiphytic bacteria are able to produce enzymes that are of biotechnological interest such as cellulases, lipases, amylases, agarases, laccases, and proteases (Comba-Gonzaĺez et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Biotechnological potential of Ulva ohnoi epiphytic bacteria: enzyme production and antimicrobial activities

et al. 2023

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Seaweed surfaces harbor diverse epibiotic bacterial communities with functions related to morphogenesis, host health, and defense. Among seaweed holobionts, culturable strains can represent innovative sources of bioactive compounds and enzymes. The global industrial demand for microbial enzymes is continually growing in order to improve certain manufacturing processes with new perspectives of industrial exploitation. In this regard, the present study focuses on the enzymatic production and the antimicrobial activities of culturable epibiotic bacteria of Ulva from the Tunisian coast. Culturable associated bacteria were isolated and molecular identification was realized by 16S rRNA gene sequencing. For each strain, eight enzymatic activities were investigated: amylase, hemolysis, DNase, cellulase, lecithinase, lipase, gelatinase, and chitinase. The antimicrobial activity of Ulva-associated bacteria was evaluated against seven pathogenic bacteria, Escherichia coli, Vibrio anguillarum, Vibrio alginoliticus, Pseudomonas aeruginosa, Aeromonas hydrophila, Salmonella typhymurium, and Staphylococcus aureus, and one yeast, Candida albicans. The antibiotic resistance of isolated strains was determined for 15 commonly used antibiotics. The phylogenetic analysis revealed that the isolates belonged to Alphaproteobacteria (3), Gammaproteobacteria (5), Actinobacteria (3), and Firmicutes (4) phyllum. The majority of the isolates (66%) produced simultaneously more than one enzyme. Hemolysis was produced by 46.6% of isolates, while DNase was produced by 33% of strains. On the other hand, 13% of strains produced lecithinase, gelatinase, cellulase, and lipase. No chitinase was produced by the isolated bacteria. In addition, 60% of isolates displayed antimicrobial activity against at least one pathogenic strain. All Ulva ohnoi-associated bacteria were resistant to at least seven commonly used antibiotics. These results highlighted the occurrence of several enzymatic activities within Ulva-associated bacteria that can have potential uses in the industrial sector.

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“…Agarases are hydrolytic enzymes found in a wide variety of marine organisms. They're used in biotechnological and commercial operations like decomposing algal polysaccharides, liquefaction of agar and agarose gels, biofilm removal in bioreactors, and the creation of simple sugars, along with in the food sector to make bread, beverages, and low-calorie foods [1] . The major component in the cell wall of red algae is agar, which is made up of agaropectin and agarose.…”

Section: Introductionmentioning

confidence: 99%

“…The creation of these polysaccharides by macroalgae encourages epiphytic bacteria to produce enzymes that can breakdown a wide range of compounds [5] . Comba-González et al, (2016) [1] constructed a flowchart illustrating the various investigational procedures intended for recognizing enzymes generated by Ulva associated-epiphytic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Agarase Production by Marine Pseudoalteromonas sp. MHS: Optimization, and Purification

Sharabash¹,

Abouelkheir²,

Mabrouk³

et al. 2022

j. of mar. sci.

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Agar is an essential polysaccharide that has been utilized in numerous fields. Many kinds of literature have been published regarding agarolytic microorganisms’ isolation and agarases biochemical studies. In this search, a local marine agarolytic bacterium associated with marine alga Ulva lactuca surface was isolated and identified as Pseudoalteromonas sp. MHS. The agarase production was parallel to the growth of Pseudoalteromonas sp. MHS as cells displayed a lag phase (2 h), subsequently an exponential growth that prolonged till 10 h where maximum growth (OD550nm = 3.9) was achieved. The enzyme activity increased rapidly as cells increased exponentially where the maximum activity of 0.22 U/mL was achieved after 8h and remained constant till 12 h during the stationary phase of growth. Agarase production was optimized using Plackett-Burman statistical design by measuring enzyme activity as a response and the design was validated using a verification experiment; the activity of the enzyme increased from 0.22 U/mL to 0.29 U/mL. Pseudoalteromonas sp. MHS agarase was partially purified and its molecular weight (MW) was determined by SDSPAGE (15-25 kDa). Agarase showed approximately 94% of its activity at 40 °C. The enzyme stability decreased as the temperature increased; the enzyme could retain about 98, 90, 80, 75, and 60% of its activity at 20, 30, 40, 50, and 60 °C, respectively. Biomass of the red alga Pterocladia capillacea proved to be a suitable substrate for agarase production using Pseudoalteromonas sp. MHS; the enzyme activity recorded after 24 h of incubation was 0.35 U/mL compared to 0.29 U/mL from the optimized medium.

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Epiphytic Bacteria of Macroalgae of the Genus Ulva and their Potential in Producing Enzymes Having Biotechnological Interest

Cited by 9 publications

References 0 publications

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

Two possible candidate enzymes from Ulva lactuca-associated epiphytic bacteria obtained through PCR and functional evaluation

Biotechnological potential of Ulva ohnoi epiphytic bacteria: enzyme production and antimicrobial activities

Agarase Production by Marine Pseudoalteromonas sp. MHS: Optimization, and Purification

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