Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity

He, Dan; Ren, Lijuan; Wu, Qinglong L.

doi:10.1007/s00343-012-1084-0

Cited by 59 publications

(33 citation statements)

References 49 publications

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“…3 and Table S3). α-Proteobacteria was the most dominant group in biofilm from Potamogeton crispus in 12 freshwater lakes of China26, while β-Proteobacteria was the most abundant class on the leaves of two submerged macrophytes in Lake Taihu28. The percentages of unclassified or unknown species at the OTU level (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…The harvested samples were transferred into a sterile 500 mL polyethylene bottle containing 400 mL of 50 mM phosphate-buffered saline (PBS, pH = 7.4) solution. Epiphytic microbes were detached after 3 min of ultra-sonication, 30 min of shaking (225 r/min) and subsequent ultra-sonication for 3 min28. The leaves were collected into a new tube containing 100 mL of PBS (pH 7.4) and treated with ultra-sonication for 3 min, followed by shaking at approximately 225 rpm for 30 min.…”

Section: Methodsmentioning

confidence: 99%

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Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

Zhang

Pang

Wang

et al. 2016

Sci Rep

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Submerged macrophytes play important roles in constructed wetlands and natural water bodies, as these organisms remove nutrients and provide large surfaces for biofilms, which are beneficial for nitrogen removal, particularly from submerged macrophyte-dominated water columns. However, information on the responses of biofilms to submerged macrophytes and nitrogen molecules is limited. In the present study, bacterial community structure and denitrifiers were investigated in biofilms on the leaves of four submerged macrophytes and artificial plants exposed to two nitrate concentrations. The biofilm cells were evenly distributed on artificial plants but appeared in microcolonies on the surfaces of submerged macrophytes. Proteobacteria was the most abundant phylum in all samples, accounting for 27.3–64.8% of the high-quality bacterial reads, followed by Chloroflexi (3.7–25.4%), Firmicutes (3.0–20.1%), Acidobacteria (2.7–15.7%), Actinobacteria (2.2–8.7%), Bacteroidetes (0.5–9.7%), and Verrucomicrobia (2.4–5.2%). Cluster analysis showed that bacterial community structure can be significantly different on macrophytes versus from those on artificial plants. Redundancy analysis showed that electrical conductivity and nitrate concentration were positively correlated with Shannon index and operational taxonomic unit (OTU) richness (log10 transformed) but somewhat negatively correlated with microbial density. The relative abundances of five denitrifying genes were positively correlated with nitrate concentration and electrical conductivity but negatively correlated with dissolved oxygen.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

Zhang

Pang

Wang

et al. 2016

Sci Rep

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“…They are also reported to be present in extreme environments such as hypersaline (Burns et al, 2004;Baumgartner et al, 2009) and acidophilic (Ivanova & Dedysh, 2012) habitats and glacial waters (Zeng et al, 2013b). Planctomycetes can also be found in association with eukaryotic hosts, like ants (Eilmus & Heil, 2009), invertebrates (Fuerst et al, 1991;Chaiyapechara et al, 2012), sponges (Pimentel-Elardo et al, 2003;Mohamed et al, 2008;Zhu et al, 2008;Ouyang et al, 2010;Sun et al, 2010;Sipkema et al, 2011;Webster et al, 2011;Costa et al, 2012), ascidians (Oliveira et al, 2013), corals (Webster & Bourne, 2007), macrophytes (Hempel et al, 2008;He et al, 2012), lichens (Grube et al, 2012), Sphagnum peat bogs (Kulichevskaya et al, 2006), and with the rhizosphere of several plants (Da Rocha et al, 2009). Several studies have shown that Planctomycetes are frequent in the epibacterial community of several macroalgae (Longford et al, 2007;Burke et al, 2011;Lachnit et al, 2011;Lage & Bondoso, 2011;de Oliveira et al, 2012;Hollants et al, 2013;Miranda et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Community composition of thePlanctomycetesassociated with different macroalgae

Bondoso

Balagué

Gasol

et al. 2014

FEMS Microbiol Ecol

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Insights into the diversity of marine natural microbial biofilms, as for example those developing at the surface of marine macroalgae, can be obtained by using molecular techniques based on 16S rRNA genes. We applied denaturing gradient gel electrophoresis (DGGE) with 16S rRNA genes-specific primers for Planctomycetes to compare the communities of these organisms developing on six different macroalgae (Chondrus crispus, Fucus spiralis, Mastocarpus stellatus, Porphyra dioica, Sargassum muticum, and Ulva sp.) sampled in spring 2012 in two rocky beaches in the north of Portugal. Planctomycetes can be one of the dominant organisms found in the epibacterial community of macroalgae, and we wanted to determine the degree of specificity and the spatial variation of these group. Shannon diversity indexes obtained from the comparison of DGGE profiles were similar in all the macroalgae, and in both sites, F. spiralis was the algae presenting lower Planctomycetes diversity, while M. stellatus and P. dioica from Porto showed the highest diversity. The analysis of DGGE profiles, including anosim statistics, indicate the existence of a specific Planctomycetes community associated with the algal host, likely independent of geographical variation. Sequencing of DGGE bands indicated that Planctomycetes communities were highly diverse, and some Operational Taxonomic Units seemed to be specifically associated with each macroalgae.

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“…Macrophytes can leach different types of dissolved organics (Bastidas Navarro et al, 2009) and inorganics (Anesio et al, 1999), which can be nutritional or detrimental to epibiotic bacteria (Hempel et al, 2008), onto their leaf surfaces. The different physical or biochemical characteristics on leaves could result in host-specific communities among different plant species (Crump & Koch, 2008;Hempel et al, 2008;Lachnit et al, 2011;He et al, 2012). Epibiotic bacteria could also be influenced directly by the surrounding water body.…”

Section: Introductionmentioning

confidence: 99%

Contrasting diversity of epibiotic bacteria and surrounding bacterioplankton of a common submerged macrophyte,Potamogeton crispus, in freshwater lakes

Ren

2014

FEMS Microbiol Ecol

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Epibiotic bacteria on surfaces of submerged macrophytes play important roles in the ecological processes of shallow lakes. However, their community ecology and dynamics are far from understood in comparison with those of bacterioplankton. Here, we conducted a comparative study of the species diversity and composition of epibiotic bacterial and the surrounding bacterioplankton communities of a common submerged macrophyte, Potamogeton crispus, in 12 lakes at a regional scale in China. We found that in different freshwater lakes, epibiotic bacteria possessed higher taxonomic richness than bacterioplankton did. There existed a marked divergence in the community structure between epibiotic bacteria and bacterioplankton. Alphaproteobacteria was the most dominant group for epibiotic bacteria, whereas Actinobacteria dominated bacterioplankton. Although variations in both bacterioplankton and epibiotic bacterial community compositions in different lakes were better explained by environmental than spatial factors, both environment and space had more intensified effects on epibiotic bacteria. This implied more complex and diverse 'microhabitats' for epibiotic bacteria on surfaces of submerged macrophytes, which may lead to higher variations of epibiotic bacteria than bacterioplankton. Our study suggested that epibiotic bacteria exhibited higher diversity and distinct community composition than the surrounding bacterioplankton. More attention should be focused on the productive and diverse microbial habitats on submerged macrophytes.

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Epiphytic bacterial communities on two common submerged macrophytes in Taihu Lake: diversity and host-specificity

Cited by 59 publications

References 49 publications

Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

Responses of bacterial community structure and denitrifying bacteria in biofilm to submerged macrophytes and nitrate

Community composition of thePlanctomycetesassociated with different macroalgae

Contrasting diversity of epibiotic bacteria and surrounding bacterioplankton of a common submerged macrophyte,Potamogeton crispus, in freshwater lakes

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