Epirubicin and meglumine γ‐linolenic acid

Harris, Neil; Anderson, William R.; Lwaleed, Bashir A.; Cooper, Alan; Birch, Brian; Solomon, Lemke

doi:10.1002/cncr.11055

Cited by 22 publications

(4 citation statements)

References 31 publications

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“…Spontaneously fluorescent epirubicin was used to examine epirubicine uptake (12,13). Forty-eight hours after siRNA transfection, 3 ϫ 10 5 cells were trypsinized and resuspended in 1 ml of medium containing 1 M Oregon Green paclitaxel or 16 M epirubicin and incubated for 20, 50, or 80 min T, paclitaxel; FAC, 5-flurouracil, doxorubicin, and cyclophosphamide.…”

Section: Methodsmentioning

confidence: 99%

Microtubule-associated protein tau: A marker of paclitaxel sensitivity in breast cancer

Rouzier

Rajan

Wagner

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

381

301

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Breast cancers show variable sensitivity to paclitaxel. There is no diagnostic test to identify tumors that are sensitive to this drug. We used U133A chips to identify genes that are associated with pathologic complete response (pCR) to preoperative paclitaxelcontaining chemotherapy in stage I-III breast cancer (n ‫؍‬ 82). Tau was the most differentially expressed gene. Tumors with pCR had significantly lower (P < 0.3 ؋ 10 ؊5 ) mRNA expression. Tissue arrays from 122 independent but similarly treated patients were used for validation by immunohistochemistry. Seventy-four percent of pCR cases were tau protein negative; the odds ratio for pCR was 3.7 (95% confidence interval, 1.6 -8.6; P ϭ 0.0013). In multivariate analysis, nuclear grade (P < 0.01), age <50 (P ϭ 0.03), and taunegative status (P ϭ 0.04) were independent predictors of pCR. Small interfering RNA experiments were performed to examine whether down-regulation of tau increases sensitivity to chemotherapy in vitro. Down-regulation of tau increased sensitivity of breast cancer cells to paclitaxel but not to epirubicin. Tubulin polymerization assay was used to assess whether tau modulates binding of paclitaxel to tubulin. Preincubation of tubulin with tau resulted in decreased paclitaxel binding and reduced paclitaxelinduced microtubule polymerization. These data suggest that low tau expression renders microtubules more vulnerable to paclitaxel and makes breast cancer cells hypersensitive to this drug. Low tau expression may be used as a marker to select patients for paclitaxel therapy. Inhibition of tau function might be exploited as a therapeutic strategy to increase sensitivity to paclitaxel. adjuvant therapy ͉ drug resistance

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Section: Methodsmentioning

confidence: 99%

Microtubule-associated protein tau: A marker of paclitaxel sensitivity in breast cancer

Rouzier

Rajan

Wagner

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

381

301

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“…The capacity of GLA to enhance the activity of various pharmaceutical preparations against cancer cells has been previously described . GLA itself was found to induce apoptosis on HL‐60 cells and to be toxic on human leukemic K562 cells, especially in high concentrations .…”

Section: Discussionmentioning

confidence: 92%

“…reported that lithium or meglumine GLA salts may exert a direct antiproliferative activity on human glioblastoma cell lines . This fatty acid was shown to enhance epirubicin efficacy on human urothelial cancer cells and tamoxifen activity in breast cancer patients , while it exerted synergistic activity with vinorelbine against breast cancer cells and amplified the sensitivity of leukemic cells to doxorubicin, etoposide, and vincristine . It seems that the growth inhibitory effect of lithium GLA on pancreatic cancer cell lines was enhanced by ferric ions but repressed by albumin .…”

Section: Introductionmentioning

confidence: 99%

Fatty acid lithium salts from Cunninghamella echinulata have cytotoxic and genotoxic effects on HL‐60 human leukemia cells

Alakhras

Bellou

Fotaki

et al. 2015

Engineering in Life Sciences

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Polyunsaturated fatty acids, especially gamma linolenic acid (GLA), are potentially useful agents in the treatment of cancer. Cunninghamella echinulata, a fungus species that is able to synthesize GLA, when cultivated under nitrogen‐limited conditions in a medium having glucose as carbon and energy source, accumulated 32–35% of lipids containing 11–18% GLA. The conversion yield of glucose to lipid was around 0.11 g per gram of glucose consumed while the lipid production was 5 g/L. Fatty acid lithium salts (FALS) were prepared from the total Cunninghamella lipids and studied for their effects on HL‐60 human leukemic cells. Cytotoxicity of FALS on HL‐60 leukemic cells was linearly related to the FALS concentration. High FALS concentration (i.e. 15 and 20 μg/mL) induced DNA fragmentation, while concurrent treatment of cells with H2O2 (at 100 μM) and FALS resulted in enhanced cytotoxicity of H2O2. However, when FALS were employed at low concentrations (i.e. 5 and 10 μg/mL), they demonstrated a protective effect on HL‐60 cells against H2O2 genotoxicity, whereas at 20 μg/mL FALS enhanced the ability of H2O2 to induce DNA fragmentation. It is concluded that FALS derived from C. echinulata lipids could be an effective preparation against HL‐60 human leukemic cells.

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“…GLA is also sometimes promoted as an anti-cancer agent. Neither GLA nor other GLA-rich supplements have been convincingly shown to be useful in preventing or treating any other health conditions [32,33,34,35]. 9-octadecynoic acid (oleic acid) is the most abundant fatty acid in human adipose tissue [36], and second in abundance in human tissues overall only to palmitic acid [37].…”

Section: Resultsmentioning

confidence: 99%

Extractions of Oil from Descurainia sophia Seed Using Supercritical CO2, Chemical Compositions by GC-MS and Evaluation of the Anti-Tussive, Expectorant and Anti-Asthmatic Activities

Gong

Zhang

et al. 2015

Molecules

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Descurainia sophia is widely distributed in China and is one of the most troublesome annual weeds. It has diverse medicinal usage. D. sophia has abundant oil, making it an important oil plant in China. The main goal of this study was to obtain the maximum yield of the oil by an optimal selection of supercritical fluid extraction parameters. According to the central composite design and response surface methodology for supercritical fluid extraction method, a quadratic polynomial model was used to predict the yield of D. sophia seed oil. A series of runs was performed to assess the optimal extraction conditions. The results indicated that the extraction pressure had the greatest impact on oil yield within the range of the operating conditions studied. A total of approximately 67 compounds were separated in D. sophia seed oil by GC-MS, of which 51 compounds represented 98.21% of the total oils, for the first time. This study was also aimed at evaluating the anti-asthmatic, anti-tussive and expectorant activities in vivo of D. sophia seed oil which supplied for further research on bioactive constituents and pharmacological mechanisms.

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Epirubicin and meglumine γ‐linolenic acid

Cited by 22 publications

References 31 publications

Microtubule-associated protein tau: A marker of paclitaxel sensitivity in breast cancer

Microtubule-associated protein tau: A marker of paclitaxel sensitivity in breast cancer

Fatty acid lithium salts from Cunninghamella echinulata have cytotoxic and genotoxic effects on HL‐60 human leukemia cells

Extractions of Oil from Descurainia sophia Seed Using Supercritical CO2, Chemical Compositions by GC-MS and Evaluation of the Anti-Tussive, Expectorant and Anti-Asthmatic Activities

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