Epithelial cells from oral mucosa: How to cultivate them?

Abstract

Epithelial cells from oral mucosa (EOM) are responsible for important functions, like the primary protection of oral mucosa against external aggressions building a mechanical barrier against microorganisms, mechanical damage, toxic material, thermal regulation and secretion of different classes of inflammatory mediators. EOM could be an interesting tool for cellular and molecular biology research. Usually, EOM are collected by a painful and invasive process. In this study, we propose an alternative method to cultivate EOM collected by non-invasive scraping method of oral mucosa. Papanicolaou staining showed mainly two kinds of epithelial cell population after EOM scraping.As result of the five culture methods tested here, our results revealed that the EOM were successfully cultured on a murine feeder layer. In addition, EOM could be frozen and thawed, without morphology changes and loss of viability. Our findings suggest that EOM can be considered as a good cell source for many purposes, such as genetic studies, diagnosis and cell therapy.

In Vitro Investigation of the Cytotoxic Effects of Different Detergent-Containing Children's Toothpastes on Human Gingival Epithelial Cells

“…In studies for the characterization of gingival epithelial cells, the method of determining epithelial cells speci c CK13 and Vimentin genes by PCR, analysis of phenotypic properties of cells by transmission electron microscopy, determination of a speci c epithelial marker cytokeratin by immuno uorescence method, staining of cells with Papanicolau staining method and analysis under light microscopy. methods were used [40]. In this study, the cells, which are easier and quicker to apply than other methods and are also more cost-effective, were stained with hematoxylin and eosin dyes after xation with alcohol on the slide, and the cells were analyzed under light microscopy, and the presence of epithelial cells was determined.…”

Novel zafirlukast derivatives exhibit selective antibacterial activity againstPorphyromonas gingivalis

“…To determine the selectivity of the zafirlukast derivatives synthesized, we tested the most active compounds 22b, 23a, and 23b against immortalized oral keratinocyte cell line OKF6, along with zafirlukast as a control. [40][41][42][43] A preliminary LIVE/DEAD assay was performed after incubating the cells with compounds 22b, 23a, and 23b for 24 h (Fig. 5).…”