Epithelial stem cells in corneal regeneration and epidermal gene therapy

Pellegrini, Graziella; Rama, Paolo; Mavilio, Fulvio; Luca, Michele De

doi:10.1002/path.2441

Cited by 102 publications

(66 citation statements)

References 116 publications

(261 reference statements)

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“…Ocular injury, trauma, congenital diseases, and autoimmune diseases could lead to partial or total deficiency in the number and function of LSCs. These conditions may also contribute to the subsequent development of corneal ulcer, opacity, and corneal neovascularization (CNV), which seriously affect ocular surface function and vision [48,49] . LSC transplantation is an effective way of treating severe LSC damage.…”

Section: Msc Mobilization and Homingmentioning

confidence: 99%

Mesenchymal stem cells: Potential role in corneal wound repair and transplantation

Li¹

2014

WJSC

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Corneal diseases are a major cause of blindness in the world. Although great progress has been achieved in the treatment of corneal diseases, wound healing after severe corneal damage and immunosuppressive therapy after corneal transplantation remain problematic. Mesenchymal stem cells (MSCs) derived from bone marrow or other adult tissues can differentiate into various types of mesenchymal lineages, such as osteocytes, adipocytes, and chondrocytes, both in vivo and in vitro . These cells can further differentiate into specific cell types under specific conditions. MSCs migrate to injury sites and promote wound healing by secreting anti-inflammatory and growth factors. In addition, MSCs interact with innate and acquired immune cells and modulate the immune response through their powerful paracrine function. Over the last decade, MSCs have drawn considerable attention because of their beneficial properties and promising therapeutic prospective. Furthermore, MSCs have been applied to various studies related to wound healing, autoimmune diseases, and organ transplantation. This review discusses the potential functions of MSCs in protecting corneal tissue and their possible mechanisms in corneal wound healing and corneal transplantation.

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Section: Msc Mobilization and Homingmentioning

confidence: 99%

Mesenchymal stem cells: Potential role in corneal wound repair and transplantation

Li¹

2014

WJSC

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“…In many specialized organs, such as the cornea, the damage of a particular source of SC can result in an irreversible loss of function of the tissue or organ and ultimately, in the case of the eye, in blindness (4,5). Such a deficiency in limbal stem cells (LSCs) may be congenital or caused by mechanical injury, various toxic substances, or harmful local inflammatory reactions.…”

mentioning

confidence: 99%

Immunoregulatory Properties of Mouse Limbal Stem Cells

Holáň

Pokorná

Procházková

et al. 2010

The Journal of Immunology

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Stem cells have been demonstrated in nearly all adult mammalian tissues and play a vital role in their physiological renewal and healing after injury. Due to their irreplaceable role in tissue repair, these cells had to develop mechanisms protecting them from deleterious inflammatory immune reactions and ensuring their increased resistance to various apoptosis-inducing agents. In this study, we demonstrate that a population of mouse limbal cells highly enriched for cells expressing markers and charateristics of limbal stem cells (LSCs) suppresses in a dose-dependent manner the proliferation of lymphocytes elicited by mitogens or TCR-triggering and significantly inhibits the production of proinflammatory cytokines by activated T cells. The suppression was mediated by soluble factor(s) and did not affect early cell activation. LSCs were even more suppressive than mesenchymal stem cells or natural regulatory T cells. In addition, the cells expressing markers and characteristics of LSC had significantly higher levels of mRNA for Fas ligand and for the antiapoptotic molecules Mcl-1, XIAP, and survivin than other limbal cell populations. LSCs were also more resistant to staurosporin-induced apoptotic cell death and to cell-mediated cytotoxic reaction than other limbal cells. Collectively, these results suggest that SC isolated from fresh adult limbal tissue possess immunomodulatory properties and inhibit proinflammatory immune reactions. Simultaneously, these cells express high levels of mRNA for antiapoptotic molecules, which can protect them against cell-mediated cytotoxic reactions and various apoptosis-inducing agents.

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“…The corneal epithelium is the peripheral contact corneal region that is characterized by dynamic turnover due to continued proliferation of its basal epithelial cells which are displaced outward by the next generation of mitotic cells, and then, they are lost by desquamation [48] . The mechanisms that regulate corneal epithelial proliferation under normal physiological or pathological conditions are usually multifactorial and complex, and they are controlled by cytokine-receptor mediated events.…”

Section: Discussionmentioning

confidence: 99%

The Potential Protective Role of Hesperidin Against Capecitabine-Induced Corneal Toxicity in Adult Male Albino Rat. Light and Electron Microscopic Study

Elwan

Kassab

2017

Egyptian Journal of Histology

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Background: Capecitabine is a chemotherapeutic agent widely used for the treatment of malignancies. Ocular disorders have been reported with capecitabine therapy. Hesperidin is a naturally occurring compound derived mainly from citrus fruits and has a wide range of pharmacological activities and a proposed role in combating many ocular diseases. Aim: To evaluate the potential protective role of hesperidin against the corneal toxicity caused by capecitabine in adult male albino rats. Material and Methods: Thirty-six adult male albino rats were divided into four equal groups; control group, hesperidintreated group (50 mg/kg), capecitabine-treated group (40 mg/kg), and combination-treated "capecitabine and hesperidintreated" group. Animals were orally administered once daily for one month. Specimens from the cornea were processed for light and electron microscopy. Immunohistochemical study was performed using antibodies against p53. Results: Specimens from capecitabine-treated animals showed significant decrease of epithelial thickness. The corneal epithelial cells showed nuclear alteration and vacuolated cytoplasm. The stromal collagen fibers were irregularlyarranged and widely separated with neovascularization and mononuclear cellular infiltration. Ultrastructurally, focal widening of the intercellular spaces, partial loss of desmosomal junctions and swollen mitochondria were observed. The immunohistochemical study showed a significant increase in p53 immunoreaction. In contrast, minimal changes were observed in rats treated concomitantly with both capecitabine and hesperidin, with a non significant increase in the immunoreactions. Conclusion: Capecitabine induced structural changes in cornea of adult albino rat that could be ameliorated by concomitant treatment with hesperidin.

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Epithelial stem cells in corneal regeneration and epidermal gene therapy

Cited by 102 publications

References 116 publications

Mesenchymal stem cells: Potential role in corneal wound repair and transplantation

Mesenchymal stem cells: Potential role in corneal wound repair and transplantation

Immunoregulatory Properties of Mouse Limbal Stem Cells

The Potential Protective Role of Hesperidin Against Capecitabine-Induced Corneal Toxicity in Adult Male Albino Rat. Light and Electron Microscopic Study

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