Epithelial stem cells in vivo

Abstract

SummaryCellular topography within the highly polarized surface epithelia can be used to identify the location of the stem cells. In some instances, this can be quite precise and allows the characteristics of stem cells to be studied. Our current knowledge of the stem cell population in murine epidermis and small intestinal crypts is reviewed. In the epidermis, the stem cells would appear to make up about 10 %

Epidermal differentiation: the bare essentials.

“…If commitment takes place in the basal layer, then the population of basal keratinocytes should be heterogeneous. Certainly, as judged by their cell cycling properties, this appears to be the case (Barrandon and Green, 1987;Potten and Morris, 1988). By this criterion, there seem to be at least two populations of basal cells.…”

“…By this criterion, there seem to be at least two populations of basal cells. One population, stem cells, have a long lifespan, a long cycling phase and short S period (for review, see Potten and Morris, 1988). The second population, transit amplifying cells or paraclones, undergo only a limited number of divisions before terminally differentiating (Barrandon and Green, 1987).…”

Single-cell expression profiling of human epidermal stem and transit-amplifying cells: Lrig1 is a regulator of stem cell quiescence

“…The bestcharacterized reservoir of SC is in the bulge of the hair follicle (3)(4)(5). However, these cells are not essential for maintenance of the interfollicular epidermis, which instead depends on its own resident SC population (6)(7)(8)(9)(10)(11).…”

“…Within the interfollicular epidermis, SC are not the only cells that are competent to proliferate (8). The progeny of a SC that are destined to terminally differentiate first undergo a few rounds of division, during which time they are known as transit-amplifying (TA) cells (12).…”

Basal cell nuclear size in experimental oral mucosal carcinogenesis

“…The basal cell population of the oral mucosa is heterogeneous and includes stem cells and amplifying or proliferating cells (Hume & Potten, 1979 (Potten & Morris, 1988). For these reasons the more mature cells of the stratum spinosum were not assessed and only basal cell nuclei were measured in the current study.…”