Epithelial stem cells, wound healing and cancer

Arwert, Esther N.; Hoste, Esther; Watt, Fiona M.

doi:10.1038/nrc3217

Cited by 399 publications

(362 citation statements)

References 139 publications

Supporting

Mentioning

337

Contrasting

Unclassified

Order By: Relevance

“…1 Upon injury, fibroblasts migrate into the wound space where they both proliferate and contribute to the formation of granulation tissue through the secretion extracellular matrix proteins including fibronectin, vitronectin and collagen. 2,3 New blood vessels form in the granulating areas (angiogenesis), and leukocytes migrate rapidly to the wound site to prevent infection. 4 Epidermal keratinocytes then migrate over the granulation tissue and proliferate to form full-thickness epidermis.…”

Section: Introductionmentioning

confidence: 99%

“…4 Epidermal keratinocytes then migrate over the granulation tissue and proliferate to form full-thickness epidermis. 2 Both keratinocytes and fibroblasts synthesize key extracellular matrix components to re-form the basement membrane beneath the wound. Some contraction due to fibroblast pulling on the extracellular matrix also facilitates human wound closure, where the associated traction forces contribute to scarring.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The integrin αv-TGFβ signaling axis is necessary for epidermal proliferation during cutaneous wound healing

et al. 2016

View full text Add to dashboard Cite

Proliferation and migration of epidermal keratinocytes are essential for proper cutaneous wound closure after injury. av integrins and several of their ligands-vitronectin, TGFb and thrombospondin-are upregulated in healing wounds. However, the role of av integrins in wound re-epithelialization is unknown. Here, we show that genetic depletion or antibody-mediated blockade of pan-integrin av, or the specific heterodimer avb6, in keratinocytes limited epidermal proliferation at the wound edge and prevented reepithelialization of wounded human organotypic skin both in vivo and in vitro. While we did not observe a migration defect upon av blockade in vivo, av was necessary for keratinocyte migration over longer distances in organotypic skin. Integrin av is required for local activation of latent TGFb, and the wound healing defect in the setting of integrin av loss was rescued by exogenous, active TGFb, indicating that the av-TGFb signaling axis is a critical component of the normal epidermal wound healing program. As chronic wounds are associated with decreased TGFb signaling, restoration of TGFb activity may have therapeutic utility in some clinical settings.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The integrin αv-TGFβ signaling axis is necessary for epidermal proliferation during cutaneous wound healing

et al. 2016

View full text Add to dashboard Cite

show abstract

“…2). Estudios más recientes, sugieren que el folículo piloso contiene otras poblaciones de células madre, algunas de ellas más activas que las LRC de la región prominente 10,11 . También se ha demostrado la presencia de células progenitoras en el estrato basal de la epidermis interfolicular.…”

Section: Células Madre Y Homeostasis Epidérmicaunclassified

“…En cualquier caso, estas células producen los linajes celulares que forman el folículo piloso, la glándula sebácea y la epidermis. Las células progenitoras de la región prominente del folículo no sólo contribuyen a la regeneración del pelo, sino que participan en la cicatrización de heridas, ya que son capaces de emigrar a la epidermis interfolicular y diferenciar a queratinocitos 10 . Esta población de células progenitoras exhibe una vigorosa multipotencia y es capaz de diferenciar in vitro, no sólo a queratinocitos, sino también a neuronas, glía, melanocitos y células mesenquimáticas 12 .…”

Section: Células Madre Y Homeostasis Epidérmicaunclassified

See 1 more Smart Citation

Nuevos modelos experimentales para el estudio de la homeostasis y la enfermedad cutánea

Larcher¹,

Espada

Díaz-Ley

et al. 2015

Actas Dermo-Sifiliográficas

View full text Add to dashboard Cite

FinanciaciónEl consorcio SkinModel-CM está financiado por la Comunidad de Madrid (programa S2010/BMD-2359). Conflicto de intereses Los autores declaran no tener ningún conflicto de intereses AgradecimientosQueremos agradecer calurosamente el trabajo realizado por los miembros y colaboradores de nuestros laboratorios que con su trabajo de cada día han hecho posible que el programa SkinModel sea una realidad.

show abstract

Differentiation of Somatic Cells, Stem Cells and Stem Cell Variants:In VitroModels

Danilenko

Coffman

Studzinski

2012

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Because conditions of culture in vitro cannot be identical to those in the living system, cell lines cultured in vitro are referred to as ‘model systems’. Not all cell types are capable of differentiation in vitro . Stem cells are of various types, including embryonic stem cells (ESC), mesenchymal stem cells (MSCs), induced pluripotent stem cells (iPSCs), cancer/leukaemia stem cells (CSCs/LSCs), etc. Procedures for studying differentiation in model systems depend on the type of culture that can be employed. Techniques employed include liquid suspension cultures, monolayer cultures and complex culture systems involving semisolid matrices or liquid overlay methods. Cells derived from various tissues can be maintained in culture known as ‘primary culture’. Only a rare human cell in a culture can undergo changes in its molecular machinery known as ‘immortalisation’ which allow it to propagate indefinitely. Recent developments include systems optimised for stem cell differentiation. Such in vitro culture techniques have applications to the study of differentiation in basic research and clinical applications. Key Concepts: Mammalian cells derived from many tissues, the subject of this article, can propagate outside of the body. Stem cells can differentiate, but provide a reservoir for development of functional cells of various types which is known as ‘lineage expansion’. Stem cells give rise to progenitor cells (PCs) which can differentiate into mature cells, but have a limited repertoire of lineages of differentiation. Mature cells can be induced to have the capabilities of stem cells and PCs by introduction of four or less transcription factors (TFs), for example, Oct4, Sox2, Klf4 and c‐Myc, under defined conditions. The cell environment – other cells, extracellular material – can favour lineage expansion into mature, functional cells and is called a ‘niche’.

show abstract

Epithelial stem cells, wound healing and cancer

Cited by 399 publications

References 139 publications

The integrin αv-TGFβ signaling axis is necessary for epidermal proliferation during cutaneous wound healing

The integrin αv-TGFβ signaling axis is necessary for epidermal proliferation during cutaneous wound healing

Nuevos modelos experimentales para el estudio de la homeostasis y la enfermedad cutánea

Differentiation of Somatic Cells, Stem Cells and Stem Cell Variants:In VitroModels

Contact Info

Product

Resources

About